Abstract
Summary Plant-parasitic nematodes limit potato production by feeding on roots, reducing tuber yield and quality, and resulting in poor growth. Dagger nematodes ( Xiphinema spp.) can pose a significant threat to crop production even in low numbers. Dagger nematodes have been reported in North Dakota, USA, potato fields. In this study, a dagger nematode species was identified and its reproduction potential in 21 potato cultivars was evaluated. The dagger nematode was identified as X. americanum via morphometric measurements and DNA sequence analysis. At the molecular level, D2-D3 of 28S ribosomal RNA (rRNA) and internal transcribed spacer (ITS)-regions of rDNA were targeted. Sequencing data of D2-D3 (749 bp) showed 98.18% similarity with three isolates of X. americanum . ITS sequence (860 bp) analysis showed 97.68% similarity with an isolate of X. americanum but lower similarity with other species of Xiphinema . The phylogenetic analysis of ITS region further confirmed the species of dagger nematode as X. americanum . Using naturally infested soil, two glasshouse experiments were conducted to assess the reproduction rate of X. americanum on 21 potato cultivars belonging to four distinct market potato classes (yellow, red, white and russet). The reproduction rates were found to vary among the potato classes and cultivars. Numerically, X. americanum population density was higher in ‘Soraya’, whereas ‘Ranger Russet’, ‘Manistee’, ‘Kennebec’, ‘Russet Norkotah 278’, ‘Modoc’, ‘Pomerelle Russet’ and ‘Dakota Rose’ reduced nematode reproduction in both trials. This is the first study demonstrating the reproduction of X. americanum in potato cultivars and could be helpful to manage dagger nematodes.
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Claims 6
Claim · model-written summary
The dagger nematode on potato was identified as X. americanum.
- Taxon
- potato
Solanum tuberosumt0 - Interactor
- X. americanumXiphinema americanume0pest
- Outcome
- identified as X. americanume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The dagger nematode was identified as X. americanum via morphometric measurements and DNA sequence analysis”
t0“potato”
Claim · model-written summary
D2-D3 sequence similarity to three X. americanum isolates was 98.18%.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- X. americanumXiphinema americanume0pest
- Outcome
- similarity with three isolates of X. americanume0
- Value
- 98.18%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequencing data of D2-D3 (749 bp) showed 98.18% similarity with three isolates of X. americanum”
t0“potato”
Claim · model-written summary
ITS sequence similarity to an X. americanum isolate was 97.68%.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- X. americanumXiphinema americanume0pest
- Outcome
- similarity with an isolate of X. americanume0
- Value
- 97.68%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ITS sequence (860 bp) analysis showed 97.68% similarity with an isolate of X. americanum”
t0“potato”
Claim · model-written summary
Nematode reproduction rates varied among potato classes and cultivars.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- Xiphinema americanumXiphinema americanumt1pest
- Outcome
- reproduction ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The reproduction rates were found to vary among the potato classes and cultivars”
t0“potato”
t1“Xiphinema americanum”
Claim · model-written summary
X. americanum population density was numerically higher in Soraya.
- Taxon
- potatoSolanum tuberosumt0
- Material
- ‘Soraya’e0
- Interactor
- X. americanumXiphinema americanume0pest
- Outcome
- X. americanum population densitye0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“X. americanum population density was higher in ‘Soraya’”
t0“potato”
Claim · model-written summary
Seven named cultivars reduced nematode reproduction in both trials.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- Xiphinema americanumXiphinema americanumt1pest
- Outcome
- nematode reproductione0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“‘Ranger Russet’, ‘Manistee’, ‘Kennebec’, ‘Russet Norkotah 278’, ‘Modoc’, ‘Pomerelle Russet’ and ‘Dakota Rose’ reduced nematode reproduction in both trials”
t0“potato”
t1“Xiphinema americanum”