Abstract
Potato virus Y infects potato plants and causes serious damage to agriculture by reducing the yield of this crop. Therefore, there is a need to identify this pathogen. Potato viruses contain RNA, so immunological methods or PCR combined with reverse transcription can be used. The aim of this work was to develop a PCR test system for the detection of Potato virus Y. Primers for the reverse transcription reaction and subsequent PCR were found. Annealing temperatures and size of the amplified fragment were calculated. Primers and reaction conditions on plant material were tested. PCR products of the calculated size were obtained. The determination of their nucleotide sequence confirmed the identification of the genetic material of Potato virus Y. Thus, this PCR test system can be used to detect Potato virus Y.
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Claims 3
Claim · model-written summary
Primers and reaction conditions tested on plant material gave PCR products of the calculated size.
Verbatim
e1“Primers and reaction conditions on plant material were tested. PCR products of the calculated size were obtained”
Claim · model-written summary
Nucleotide sequencing of the PCR products confirmed identification of the genetic material of Potato virus Y.
- Taxon
- Potato virus Y
Potato virus Ye1 - Interactor
- Potato virus YPotato virus Ye1pathogen
- Outcome
- identification of the genetic materiale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The determination of their nucleotide sequence confirmed the identification of the genetic material of Potato virus Y”
Claim · model-written summary
The authors conclude that this PCR test system can be used to detect Potato virus Y.
- Taxon
- Potato virus YPotato virus Ye1
- Interactor
- Potato virus YPotato virus Ye1pathogen
- Outcome
- detect Potato virus Ye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“this PCR test system can be used to detect Potato virus Y”