Abstract
Natural resistance-associated macrophage proteins (NRAMPs) are a class of metal transporters found in plants that exhibit diverse functions across different species. Transporter proteins facilitate the absorption, distribution, and sequestration of metallic elements within various plant tissues. Despite the extensive identification of NRAMP family genes in various species, a full analysis of these genes in tree species is still necessary. Genome-wide identification and bioinformatics analysis were performed to understand the roles of NRAMP genes in copper (CuCl 2 ) stress in Kandelia obovata (Ko). In Arachis hypogaea L., Populus trichocarpa , Vitis vinifera , Phaseolus vulgaris L., Camellia sinensis , Spirodela polyrhiza , Glycine max L. and Solanum lycopersicum , a genome-wide study of the NRAMP gene family was performed earlier. The domain and 3D structural variation, phylogenetic tree, chromosomal distributions, gene structure, motif analysis, subcellular localization, cis-regulatory elements, synteny and duplication analysis, and expression profiles in leaves and CuCl 2 were all investigated in this research. In order to comprehend the notable functions of the NRAMP gene family in Kandelia obovata , a comprehensive investigation was conducted at the genomic level. This study successfully found five NRAMP genes, encompassing one gene pair resulting from whole-genome duplication and a gene that had undergone segmental duplication. The examination of chromosomal position revealed an unequal distribution of the KoNRAMP genes across chromosomes 1, 2, 5, 7, and 18. The KoNRAMPs can be classified into three subgroups (I, II, and SLC) based on phylogeny and synteny analyses, similar to Solanum lycopersicum . Examining cis-regulatory elements in the promoters revealed five hormone-correlated responsive elements and four stress-related responsive elements. The genomic architecture and properties of 10 highly conserved motifs are similar among members of the NRAMP gene family. The conducted investigations demonstrated that the expression levels of all five genes exhibited alterations in response to different levels of CuCl 2 stress. The results of this study offer crucial insights into the roles of KoNRAMPs in the response of Kandelia obovata to CuCl 2 stress.
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Claims 8
Claim · model-written summary
Five NRAMP genes were identified in Kandelia obovata.
- Taxon
- Kandelia obovata
Kandelia obovatat0 - Gene
- NRAMP genese0
- Outcome
- five NRAMP genese0
- Value
- five NRAMP genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study successfully found five NRAMP genes”
t0“Kandelia obovata”
Claim · model-written summary
One NRAMP gene pair arose from whole-genome duplication.
- Taxon
- Kandelia obovataKandelia obovatat0
- Gene
- gene paire0
- Outcome
- one gene pair resulting from whole-genome duplicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one gene pair resulting from whole-genome duplication”
t0“Kandelia obovata”
Claim · model-written summary
One NRAMP gene had undergone segmental duplication.
- Taxon
- Kandelia obovataKandelia obovatat0
- Gene
- a genee0
- Outcome
- undergone segmental duplicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a gene that had undergone segmental duplication”
t0“Kandelia obovata”
Claim · model-written summary
KoNRAMP genes were distributed unequally across chromosomes 1, 2, 5, 7 and 18.
- Taxon
- Kandelia obovataKandelia obovatat0
- Gene
- KoNRAMP genese0
- Outcome
- unequal distribution of the KoNRAMP genes across chromosomes 1, 2, 5, 7, and 18e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“unequal distribution of the KoNRAMP genes across chromosomes 1, 2, 5, 7, and 18”
t0“Kandelia obovata”
Claim · model-written summary
KoNRAMP genes were classified into three phylogenetic and syntenic subgroups.
- Taxon
- Kandelia obovataKandelia obovatat0
- Gene
- KoNRAMPse0
- Outcome
- three subgroups (I, II, and SLC)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The KoNRAMPs can be classified into three subgroups (I, II, and SLC) based on phylogeny and synteny analyses”
t0“Kandelia obovata”
Claim · model-written summary
KoNRAMP promoters contained five hormone-correlated response elements.
- Taxon
- Kandelia obovataKandelia obovatat0
- Outcome
- five hormone-correlated responsive elementse0
- Value
- five hormone-correlated responsive elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Examining cis-regulatory elements in the promoters revealed five hormone-correlated responsive elements and four stress-related responsive elements.”
t0“Kandelia obovata”
Claim · model-written summary
KoNRAMP promoters contained four stress-related response elements.
- Taxon
- Kandelia obovataKandelia obovatat0
- Outcome
- four stress-related responsive elementse0
- Value
- four stress-related responsive elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Examining cis-regulatory elements in the promoters revealed five hormone-correlated responsive elements and four stress-related responsive elements.”
t0“Kandelia obovata”
Claim · model-written summary
Ten highly conserved motifs had similar architecture and properties among NRAMP family members.
- Taxon
- Kandelia obovataKandelia obovatat0
- Gene
- NRAMP gene familye0
- Outcome
- genomic architecture and properties of 10 highly conserved motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genomic architecture and properties of 10 highly conserved motifs are similar among members of the NRAMP gene family.”
t0“Kandelia obovata”