Abstract
Neuwiedia singapureana is a rare and endangered plant of the Apostasioideae subfamily. The Apostasioideae subfamily has a unique evolutionary status, as it is considered to be the most primitive group forming the base of the Orchidaceae evolutionary tree. Therefore, N. singapureana has high scientific research and conservation value. The endophytic fungal communities associated with orchids are rich and diverse, but few studies have investigated the endophytic fungi of Neuwiedia orchid plants. In the present study, the aim was to examine the endophytic fungal community structures associated with wild N. singapureana rhizomes and normal roots in the ground and with bare prop roots in the air at two sampling sites in China. High-throughput sequencing of nuclear ribosomal DNA fragments of the internal transcribed spacer regions was conducted, and cultivable methods were adopted. A total of 2161 endophytic fungal operational taxonomic units (OTUs) were obtained at a 97% sequence similarity threshold. The endophytic fungal diversity differed among the samples but not significantly. There were many more non-mycorrhizal endophytic fungal than orchid mycorrhizal (OM) fungal species detected in the N. singapureana orchid, about 98.33% OTUs of non-mycorrhizal fungi contrasting with 1.67% OTUs of potential orchid mycorrhizal fungi, among which Ceratobasidiaceae, Russulaceae, and Thelephoraceae were the dominant orchid mycorrhizal fungi. One culturable OM fungal Epulorhiza sp. isolated from the rhizome was capable of significantly promoting the seed germination and seedling growth of Dendrobium officinale and Epidendrum secundum orchids, respectively, with different efficiencies. These endophytic fungal strains with growth-promoting functions will provide materials for orchid conservation and for the study of the mechanisms underlying orchid symbiotic associations.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Sequencing found 2161 endophytic fungal OTUs.
- Taxon
- N. singapureanaNeuwiedia zollingeri var. singapureanae1
- Outcome
- endophytic fungal operational taxonomic units (OTUs)e0
- Value
- 2161e0
- Qualifier
- 97% sequence similarity thresholde0
Verbatim
e0“A total of 2161 endophytic fungal operational taxonomic units (OTUs) were obtained at a 97% sequence similarity threshold.”
e1“N. singapureana orchid”
Claim · model-written summary
Endophytic fungal diversity differed among samples without statistical significance.
- Taxon
- N. singapureanaNeuwiedia zollingeri var. singapureanae1
- Outcome
- endophytic fungal diversitye0
- Direction
- differede0
Verbatim
e0“The endophytic fungal diversity differed among the samples but not significantly.”
e1“N. singapureana orchid”
Claim · model-written summary
Non-mycorrhizal fungi accounted for 98.33% of OTUs.
- Taxon
- N. singapureanaNeuwiedia zollingeri var. singapureanae1
- Outcome
- OTUs of non-mycorrhizal fungie0
- Value
- 98.33%e0
Verbatim
e0“about 98.33% OTUs of non-mycorrhizal fungi contrasting with 1.67% OTUs of potential orchid mycorrhizal fungi”
e1“N. singapureana orchid”
Claim · model-written summary
Potential orchid mycorrhizal fungi accounted for 1.67% of OTUs.
- Taxon
- N. singapureanaNeuwiedia zollingeri var. singapureanae1
- Outcome
- OTUs of potential orchid mycorrhizal fungie0
- Value
- 1.67%e0
Verbatim
e0“about 98.33% OTUs of non-mycorrhizal fungi contrasting with 1.67% OTUs of potential orchid mycorrhizal fungi”
e1“N. singapureana orchid”
Claim · model-written summary
Ceratobasidiaceae, Russulaceae, and Thelephoraceae dominated the orchid mycorrhizal fungi.
- Taxon
- N. singapureanaNeuwiedia zollingeri var. singapureanae1
- Interactor
- CeratobasidiaceaeCeratobasidiaceaee0symbiont
- Second interactor
- RussulaceaeRussulaceaee0symbiont
- Outcome
- dominant orchid mycorrhizal fungie0
- Qualifier
- Thelephoraceaee0
Verbatim
e0“Ceratobasidiaceae, Russulaceae, and Thelephoraceae were the dominant orchid mycorrhizal fungi”
e1“N. singapureana orchid”
Claim · model-written summary
Epulorhiza sp. significantly promoted Dendrobium officinale seed germination.
- Taxon
- Dendrobium officinale
_Kimura_%26_Migo%2C_J._Shanghai_Sci._Inst._3_122_(1936)%2C_nom._cons_(46810867685).jpg?width=60)
Dendrobium officinalee0 - Treatment
- Epulorhiza sp.e0
- Interactor
- Epulorhiza sp.
Tulasnellae0symbiont - Outcome
- seed germinatione0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Kimura_%26_Migo%2C_J._Shanghai_Sci._Inst._3_122_(1936)%2C_nom._cons_(46810867685).jpg?width=500)
Verbatim
e0“One culturable OM fungal Epulorhiza sp. isolated from the rhizome was capable of significantly promoting the seed germination and seedling growth of Dendrobium officinale and Epidendrum secundum orchids, respectively, with different efficiencies.”
Claim · model-written summary
Epulorhiza sp. significantly promoted Epidendrum secundum seedling growth.
- Taxon
- Epidendrum secundum
Epidendrum secundume0 - Treatment
- Epulorhiza sp.e0
- Interactor
- Epulorhiza sp.Tulasnellae0symbiont
- Outcome
- seedling growthe0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One culturable OM fungal Epulorhiza sp. isolated from the rhizome was capable of significantly promoting the seed germination and seedling growth of Dendrobium officinale and Epidendrum secundum orchids, respectively, with different efficiencies.”