Abstract
The genus Tripterygium was of great medicinal value and attracted much attention on the taxonomic study using morphological and molecular methods. In this study, we assembled 12 chloroplast genomes of Tripterygium to reveal interspecific difference and intraspecific variation. The sequence length (156,692–157,061 bp) and structure of Tripterygium were conserved. Comparative analyses presented abundant variable regions for further study. Meanwhile, we determined the ndhB gene under positive selection through adaptive evolution analysis. And the phylogenetic analyses based on 15 chloroplast genomes supported the monophyly of Tripterygium hypoglaucum and the potential sister relationship between Tripterygium wilfordii and Tripterygium regelii . Molecular dating analysis indicated that the divergence time within Tripterygium was approximately 5.99 Ma (95% HPD = 3.11–8.68 Ma). The results in our study provided new insights into the taxonomy, evolution process, and phylogenetic construction of Tripterygium using complete plastid genomes.
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Claims 7
Claim · model-written summary
Twelve Tripterygium chloroplast genomes were assembled.
- Taxon
- Tripterygium
Tripterygiume0 - Outcome
- chloroplast genomese0
- Value
- 12 chloroplast genomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we assembled 12 chloroplast genomes of Tripterygium”
Claim · model-written summary
Tripterygium chloroplast genome length and structure were conserved.
- Taxon
- TripterygiumTripterygiume0
- Outcome
- sequence lengthe0
- Value
- 156,692–157,061 bpe0
- Qualifier
- structuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequence length (156,692–157,061 bp) and structure of Tripterygium were conserved.”
Claim · model-written summary
Comparative analysis found abundant variable regions.
- Taxon
- TripterygiumTripterygiumt0
- Outcome
- abundant variable regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tripterygium genus”
e0“Comparative analyses presented abundant variable regions for further study.”
Claim · model-written summary
The ndhB gene showed positive selection.
- Taxon
- TripterygiumTripterygiumt0
- Gene
- ndhBe0
- Outcome
- positive selectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tripterygium genus”
e0“we determined the ndhB gene under positive selection through adaptive evolution analysis.”
Claim · model-written summary
Phylogeny supported monophyly of Tripterygium hypoglaucum.
- Taxon
- Tripterygium hypoglaucum
Tripterygium hypoglaucume0 - Outcome
- monophylye0
- Qualifier
- 15 chloroplast genomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the phylogenetic analyses based on 15 chloroplast genomes supported the monophyly of Tripterygium hypoglaucum”
Claim · model-written summary
Phylogeny indicated a potential sister relationship between T. wilfordii and T. regelii.
- Taxon
- Tripterygium wilfordii
Tripterygium wilfordiie0 - Outcome
- potential sister relationshipe0
- Comparator
- Tripterygium regeliie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the potential sister relationship between Tripterygium wilfordii and Tripterygium regelii”
Claim · model-written summary
Tripterygium divergence was dated to approximately 5.99 Ma.
- Taxon
- TripterygiumTripterygiume0
- Outcome
- divergence timee0
- Value
- 5.99 Mae0
- Qualifier
- 95% HPD = 3.11–8.68 Mae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Molecular dating analysis indicated that the divergence time within Tripterygium was approximately 5.99 Ma (95% HPD = 3.11–8.68 Ma).”