Abstract
Rice false smut disease is one of the most significant rice diseases worldwide. Ustilaginoidea virens is the causative agent of this disease. Although several developmental and pathogenic genes have been identified and functionally analyzed, the pathogenic molecular mechanisms of U. virens remain elusive. The velvet family regulatory proteins are involved in fungal development, conidiation, and pathogenicity. In this study, we demonstrated the function of the VelC homolog UvVELC in U. virens. We identified the velvet family protein UvVELC and characterized its functions using a target gene deletion-strategy. Deletion of UvVELC resulted in conidiation failure and pathogenicity. The UvVELC expression levels during infection suggested that this gene might be involved in the early infection process. UvVELC is also important in resistance to abiotic stresses, the utilization efficiency of glucose, stachyose, raffinose, and other sugars, and the expression of transport-related genes. Moreover, UvVELC could physically interact with UvVEA in yeast, and UvVELC/UvVEA double-knockout mutants also failed in conidiation and pathogenicity. These results indicate that UvVELC play a critical role in the conidiation and pathogenicity in U. virens. Functional analysis indicated that UvVELC-mediated conidiation and nutrient acquisition from rice regulates the pathogenicity of U. virens. Understanding the function of the UvVELC homolog could provide a potential molecular target for controlling rice false smut disease.
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Claims 8
Claim · model-written summary
Deletion of UvVELC caused failure of fungal conidiation.
- Taxon
- rice
Oryza sativat0 - Treatment
- Deletion of UvVELCe0
- Interactor
- Ustilaginoidea virens
Ustilaginoidea virenst0pathogen - Gene
- UvVELCe0
- Outcome
- conidiation failuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of UvVELC resulted in conidiation failure and pathogenicity”
t0“rice false smut pathogen Ustilaginoidea virens”
Claim · model-written summary
Deletion of UvVELC affected pathogenicity.
- Taxon
- riceOryza sativat0
- Treatment
- Deletion of UvVELCe0
- Interactor
- Ustilaginoidea virensUstilaginoidea virenst0pathogen
- Gene
- UvVELCe0
- Outcome
- pathogenicitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of UvVELC resulted in conidiation failure and pathogenicity”
t0“rice false smut pathogen Ustilaginoidea virens”
Claim · model-written summary
UvVELC expression during infection suggested an early infection role.
- Taxon
- riceOryza sativat0
- Interactor
- Ustilaginoidea virensUstilaginoidea virenst0pathogen
- Gene
- UvVELCe0
- Outcome
- early infection processe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The UvVELC expression levels during infection suggested that this gene might be involved in the early infection process”
t0“rice false smut pathogen Ustilaginoidea virens”
Claim · model-written summary
UvVELC was important for fungal abiotic-stress resistance and sugar utilization.
- Taxon
- riceOryza sativat0
- Gene
- UvVELCe0
- Outcome
- resistance to abiotic stresses, the utilization efficiency of glucose, stachyose, raffinose, and other sugarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UvVELC is also important in resistance to abiotic stresses, the utilization efficiency of glucose, stachyose, raffinose, and other sugars, and the expression of transport-related genes”
t0“rice false smut pathogen Ustilaginoidea virens”
Claim · model-written summary
UvVELC affected transport-related gene expression.
- Taxon
- riceOryza sativat0
- Gene
- UvVELCe0
- Outcome
- expression of transport-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UvVELC is also important in resistance to abiotic stresses, the utilization efficiency of glucose, stachyose, raffinose, and other sugars, and the expression of transport-related genes”
t0“rice false smut pathogen Ustilaginoidea virens”
Claim · model-written summary
UvVELC physically interacted with UvVEA in yeast.
- Taxon
- riceOryza sativat0
- Gene
- UvVELCe0
- Outcome
- physically interact with UvVEAe0
- Setting
- yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UvVELC could physically interact with UvVEA in yeast”
t0“rice false smut pathogen Ustilaginoidea virens”
Claim · model-written summary
Double knockout of UvVELC and UvVEA eliminated conidiation.
- Taxon
- riceOryza sativat0
- Treatment
- UvVELC/UvVEA double-knockout mutantse0
- Interactor
- Ustilaginoidea virensUstilaginoidea virenst0pathogen
- Gene
- UvVELC/UvVEAe0
- Outcome
- failed in conidiatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UvVELC/UvVEA double-knockout mutants also failed in conidiation and pathogenicity”
t0“rice false smut pathogen Ustilaginoidea virens”
Claim · model-written summary
Double knockout of UvVELC and UvVEA eliminated pathogenicity.
- Taxon
- riceOryza sativat0
- Treatment
- UvVELC/UvVEA double-knockout mutantse0
- Interactor
- Ustilaginoidea virensUstilaginoidea virenst0pathogen
- Gene
- UvVELC/UvVEAe0
- Outcome
- failed in conidiation and pathogenicitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UvVELC/UvVEA double-knockout mutants also failed in conidiation and pathogenicity”
t0“rice false smut pathogen Ustilaginoidea virens”