Paper · 2023 · Revista Colombiana de Ciencias Hortícolas

Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators

Beatriz Porras-García; Elberth Hernado Pinzón-Sandoval; Pedro José Almanza-Merchán

4
claims
16
verbatim quotes
2
citations
2
research areas
✓
Open access · cc-by-nc-sa

Abstract

Cannabis sativa (L.) is an annual herbaceous species which has been extensively cultivated and used as a medicinal plant and for recreational purposes. Its medicinal value is primarily attributed to a group of secondary metabolites called phytocannabinoids, which are mainly concentrated in the essential oils of unfertilized female flowers. Asexual reproduction allows for the offspring of plant material while maintaining the genetic characteristics of the mother plant. The aim of the research was to improve the vegetative propagation process of Cannabis sativa (L.) cv. Eco-2, which has characteristics of medicinal phytocannabinoid producers, using auxin plant growth regulators (NAA and IBA) at different doses. The study was conducted under greenhouse conditions in the municipality of Pesca (Colombia). Doses of 400, 800 and 1,000 mg L-1 and a control without application were evaluated for plant height, stem diameter, number of branches, root volume and length, and rooting percentage. The best treatment was obtained with the application of NAA at a dose of 800 mg L-1, which resulted in a rooting percentage of 86.6%, a root length and volume of 9.0 cm and 5.0 cm3, and an increase in the length of the cutting by 3.8 cm, with significant differences compared to the treatment without application, after 4 weeks of planting. The use of auxin plant growth regulators becomes an alternative with high potential for the propagation of medicinal cannabinoid-producing plants.

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Claims 4

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

NAA at 800 mg L-1 produced 86.6% rooting after four weeks, significantly outperforming no application.

Taxon
Cannabis sativa (L.)Cannabis sativat0
Material
Eco-2e1
Treatment
NAA at a dose of 800 mg L-1e0
Outcome
rooting percentagee0
Direction
beste0
Value
86.6%e0
Comparator
treatment without applicatione0
Setting
greenhouse conditionse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The best treatment was obtained with the application of NAA at a dose of 800 mg L-1, which resulted in a rooting percentage of 86.6%, a root length and volume of 9.0 cm and 5.0 cm3, and an increase in the length of the cutting by 3.8 cm, with significant differences compared to the treatment without application, after 4 weeks of planting.”

✓ located verbatim in the source · abstract, characters 997–1337 · show in text ↑
e1

“Cannabis sativa (L.) cv. Eco-2”

✓ located verbatim in the source · abstract, characters 550–580 · show in text ↑
e2

“greenhouse conditions”

✓ located verbatim in the source · abstract, characters 749–770 · show in text ↑
t0

“Cannabis sativa (L.) plants”

✓ located verbatim in the source · title, characters 15–42 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

NAA at 800 mg L-1 yielded a root length of 9.0 cm after four weeks, with significant differences from no application.

Taxon
Cannabis sativa (L.)Cannabis sativat0
Treatment
NAA at a dose of 800 mg L-1e0
Outcome
root lengthe0
Direction
beste0
Value
9.0 cme0
Comparator
treatment without applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The best treatment was obtained with the application of NAA at a dose of 800 mg L-1, which resulted in a rooting percentage of 86.6%, a root length and volume of 9.0 cm and 5.0 cm3, and an increase in the length of the cutting by 3.8 cm, with significant differences compared to the treatment without application, after 4 weeks of planting.”

✓ located verbatim in the source · abstract, characters 997–1337 · show in text ↑
e1

“Cannabis sativa (L.) cv. Eco-2”

✓ located verbatim in the source · abstract, characters 550–580 · show in text ↑
e2

“greenhouse conditions”

✓ located verbatim in the source · abstract, characters 749–770 · show in text ↑
t0

“Cannabis sativa (L.) plants”

✓ located verbatim in the source · title, characters 15–42 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

NAA at 800 mg L-1 yielded a root volume of 5.0 cm3 after four weeks, with significant differences from no application.

Taxon
Cannabis sativa (L.)Cannabis sativat0
Treatment
NAA at a dose of 800 mg L-1e0
Outcome
volumee0
Direction
beste0
Value
5.0 cm3e0
Comparator
treatment without applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The best treatment was obtained with the application of NAA at a dose of 800 mg L-1, which resulted in a rooting percentage of 86.6%, a root length and volume of 9.0 cm and 5.0 cm3, and an increase in the length of the cutting by 3.8 cm, with significant differences compared to the treatment without application, after 4 weeks of planting.”

✓ located verbatim in the source · abstract, characters 997–1337 · show in text ↑
e1

“Cannabis sativa (L.) cv. Eco-2”

✓ located verbatim in the source · abstract, characters 550–580 · show in text ↑
e2

“greenhouse conditions”

✓ located verbatim in the source · abstract, characters 749–770 · show in text ↑
t0

“Cannabis sativa (L.) plants”

✓ located verbatim in the source · title, characters 15–42 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

NAA at 800 mg L-1 increased cutting length by 3.8 cm after four weeks compared with no application.

Taxon
Cannabis sativa (L.)Cannabis sativat0
Treatment
NAA at a dose of 800 mg L-1e0
Outcome
length of the cuttinge0
Direction
increasee0
Value
3.8 cme0
Comparator
treatment without applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The best treatment was obtained with the application of NAA at a dose of 800 mg L-1, which resulted in a rooting percentage of 86.6%, a root length and volume of 9.0 cm and 5.0 cm3, and an increase in the length of the cutting by 3.8 cm, with significant differences compared to the treatment without application, after 4 weeks of planting.”

✓ located verbatim in the source · abstract, characters 997–1337 · show in text ↑
e1

“Cannabis sativa (L.) cv. Eco-2”

✓ located verbatim in the source · abstract, characters 550–580 · show in text ↑
e2

“greenhouse conditions”

✓ located verbatim in the source · abstract, characters 749–770 · show in text ↑
t0

“Cannabis sativa (L.) plants”

✓ located verbatim in the source · title, characters 15–42 · show in text ↑

1-naphthaleneacetic acid · growth · Cannabis sativaResults agree

10 papers: 10 positive, 0 negative or no effect

9 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.