Abstract
Leaf spot disease caused by Alternaria perpunctulata (E.G. Simmons) poses a significant threat to Alternanthera philoxeroides (Mart.) Griseb, commonly known as alligator weed and Ceylon Spinach, contributes to persistent weed proliferation in Indian paddy fields. This study focuses on the molecular identification and phylogenetic analysis of the Alternaria perpunctulata strain GVKNSV7, which is responsible for leaf spot disease on A. philoxeroides. Diseased plant leaves were collected from Kakumanu Mandal, Guntur, and local vegetable markets of Visakhapatnam, Andhra Pradesh, India, in December 2021, revealing symptomatic samples with pink to reddish spots on A. philoxeroides leaves. Employing a comprehensive approach that included morphological assessment, pathogenicity testing, and ITS region sequencing, the isolated fungus was conclusively identified as A. perpunctulata with a high sequence similarity of 98.54%. Molecular comparison with sequences in the NCBI database further validated this identification. The resulting genetic sequence, formally catalogued as "Alternaria perpunctulata GVKNSV7," with the GenBank accession number OQ073752, has been successfully submitted to the NCBI database. This study emphasized the utility of ITS sequencing and molecular analyses for accurately identifying and documenting emerging pathogens. Such documentation provides essential insights for developing future bioherbicides and effective weed management strategies. Furthermore, it highlights the potential use of A. philoxeroides as a leafy vegetable, contributing to food security and agricultural sustainability. Overall, this research contributes to understanding Alternaria perpunctulata and its implications for crop health and management practices.
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Claims 3
Claim · model-written summary
Collected A. philoxeroides leaves showed pink to reddish spots.
- Taxon
- A. philoxeroides
Alternanthera philoxeroidese0 - Organ / stage
- leavese0
- Outcome
- pink to reddish spotse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealing symptomatic samples with pink to reddish spots on A. philoxeroides leaves.”
Claim · model-written summary
The isolated fungus was identified as A. perpunctulata with 98.54% sequence similarity.
- Taxon
- Alternanthera philoxeroidesAlternanthera philoxeroidest0
- Interactor
- A. perpunctulataAlternaria perpunctulatae0pathogen
- Outcome
- sequence similaritye0
- Value
- 98.54%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the isolated fungus was conclusively identified as A. perpunctulata with a high sequence similarity of 98.54%.”
t0“Alternanthera philoxeroides”
Claim · model-written summary
The pathogen sequence was submitted to NCBI as accession OQ073752.
- Taxon
- Alternanthera philoxeroidesAlternanthera philoxeroidest0
- Interactor
- Alternaria perpunctulata GVKNSV7Alternaria perpunctulatae0pathogen
- Outcome
- genetic sequencee0
- Qualifier
- OQ073752e0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resulting genetic sequence, formally catalogued as "Alternaria perpunctulata GVKNSV7," with the GenBank accession number OQ073752, has been successfully submitted to the NCBI database.”
t0“Alternanthera philoxeroides”