Abstract
Among the many diseases that affect potato plants, viral infections are the most common and cause significant damage to farms, affecting both the yield and quality of potatoes. In this regard, an important condition for preserving the potato seed fund in Russia is systematic monitoring and early highly specific detection of potato viral infections. The purpose of the work is to study samples of potato varieties collected in the Novosibirsk region for the presence of viral infections using RT-PCR. 130 potato plants from three districts of the Novosibirsk region (NR) were studied. As a result of monitoring, the following viruses were identified: PVY (potato virus Y), PVS (potato virus S), PVM (potato virus M) and PVX (potato virus X). The quarantine pathogen potato spindle tuber viroid (PSTVd) was not detected in any of the samples analyzed. The maximum frequency of occurrence in the region was noted for three viruses: PVY, PVM and PVS. A significant proportion of the samples were mixed viral infections: the occurrence of the combination of infection PVY + PVM in plants was 25.0 %, and PVY + PVS, 22.6 %. To develop methods for determining the strain affiliation of the studied samples, the nucleotide sequences of the capsid protein genes of 10 Y-virus isolates were sequenced. Phylogenetic analysis of the studied sequences of NR isolates was carried out with a set of sequences of reference strains 261-4, Eu-N, N:O, NE-11, NTNa, NTNb, N-Wi, O, O5, SYR_I, SYR_II and SYR_III retrieved from GenBank. As a result of phylogenetic analysis, it was established that NR viral samples fell into two groups of strains: group 1, which also includes isolates of the reference strains 261-4/SYR_III, and group 2, NTNa. The obtained results of the strain affiliation of NR samples lay the basis for the development of DNA and immunodiagnostic systems for identifying PVY circulating in NR, as well as for elucidating the source and routes of entry of specific virus strains.Key words: Solanum tuberosum; viral infections; RT-PCR; potato Y virus; phylogenetic analysis.
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Claims 13
Claim · model-written summary
PVY was identified in the potato survey.
- Taxon
- potato virus Y
Potato virus Ye0 - Outcome
- identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As a result of monitoring, the following viruses were identified: PVY (potato virus Y), PVS (potato virus S), PVM (potato virus M) and PVX (potato virus X).”
Claim · model-written summary
PVS was identified in the potato survey.
- Taxon
- potato virus SPotato virus Se0
- Outcome
- identifiede0
Verbatim
e0“As a result of monitoring, the following viruses were identified: PVY (potato virus Y), PVS (potato virus S), PVM (potato virus M) and PVX (potato virus X).”
Claim · model-written summary
PVM was identified in the potato survey.
- Taxon
- potato virus MPotato virus Me0
- Outcome
- identifiede0
Verbatim
e0“As a result of monitoring, the following viruses were identified: PVY (potato virus Y), PVS (potato virus S), PVM (potato virus M) and PVX (potato virus X).”
Claim · model-written summary
PVX was identified in the potato survey.
- Taxon
- potato virus XPotato virus Xe0
- Outcome
- identifiede0
Verbatim
e0“As a result of monitoring, the following viruses were identified: PVY (potato virus Y), PVS (potato virus S), PVM (potato virus M) and PVX (potato virus X).”
Claim · model-written summary
Potato spindle tuber viroid was not detected in any analyzed sample.
- Taxon
- potato spindle tuber viroid
Potato spindle tuber viroide0 - Outcome
- not detectede0
- Qualifier
- any of the samples analyzede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The quarantine pathogen potato spindle tuber viroid (PSTVd) was not detected in any of the samples analyzed.”
Claim · model-written summary
PVY had a maximum regional occurrence frequency.
- Taxon
- PVYPotato virus Ye0
- Outcome
- frequency of occurrencee0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum frequency of occurrence in the region was noted for three viruses: PVY, PVM and PVS.”
Claim · model-written summary
PVM had a maximum regional occurrence frequency.
- Taxon
- PVMPotato virus Me0
- Outcome
- frequency of occurrencee0
- Direction
- maximume0
Verbatim
e0“The maximum frequency of occurrence in the region was noted for three viruses: PVY, PVM and PVS.”
Claim · model-written summary
PVS had a maximum regional occurrence frequency.
Verbatim
e0“The maximum frequency of occurrence in the region was noted for three viruses: PVY, PVM and PVS.”
Claim · model-written summary
PVY plus PVM infection occurred in 25.0 % of plants.
- Taxon
- potato
Solanum tuberosumt0 - Interactor
- PVYPotato virus Ye0pathogen
- Second interactor
- PVMPotato virus Me0pathogen
- Outcome
- occurrence of the combination of infection PVY + PVMe0
- Value
- 25.0 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the occurrence of the combination of infection PVY + PVM in plants was 25.0 %”
t0“potato”
Claim · model-written summary
PVY plus PVS infection occurred in 22.6 % of plants.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- PVYPotato virus Ye0pathogen
- Second interactor
- PVSe0pathogen
- Outcome
- PVY + PVSe0
- Value
- 22.6 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the occurrence of the combination of infection PVY + PVM in plants was 25.0 %, and PVY + PVS, 22.6 %.”
t0“potato”
Claim · model-written summary
Regional viral samples were placed in strain group 1 including 261-4/SYR_III.
- Taxon
- PVYPotato virus Ye1
- Outcome
- group 1e0
- Qualifier
- reference strains 261-4/SYR_IIIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As a result of phylogenetic analysis, it was established that NR viral samples fell into two groups of strains: group 1, which also includes isolates of the reference strains 261-4/SYR_III, and group 2, NTNa.”
e1“identifying PVY circulating in NR”
Claim · model-written summary
Regional viral samples were placed in strain group 2, NTNa.
- Taxon
- PVYPotato virus Ye1
- Outcome
- group 2, NTNae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As a result of phylogenetic analysis, it was established that NR viral samples fell into two groups of strains: group 1, which also includes isolates of the reference strains 261-4/SYR_III, and group 2, NTNa.”
e1“identifying PVY circulating in NR”
Claim · model-written summary
The strain affiliation results provide a basis for diagnostic development and elucidating virus entry sources and routes.
- Taxon
- PVYPotato virus Ye0
- Outcome
- development of DNA and immunodiagnostic systemse0
- Qualifier
- elucidating the source and routes of entry of specific virus strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The obtained results of the strain affiliation of NR samples lay the basis for the development of DNA and immunodiagnostic systems for identifying PVY circulating in NR, as well as for elucidating the source and routes of entry of specific virus strains.”