Abstract
Abstract BACKGROUND Sparse leaf patch (SLP) is one of the most significant diseases affecting seashore paspalum ( Paspalum vaginatum Sw.), caused by Microdochium paspali . Fast and accurate detection of this pathogen is crucial for effective disease management. However, conventional culture‐based methods are time‐consuming and often compromised by the presence of other saprophytic or endophytic fungi. RESULTS In this study, we developed a real‐time fluorescent quantitative (q)PCR method based on the internal transcribed spacer (ITS) region of the ribosomal RNA gene to rapidly detect and quantify M. paspali . The qPCR assay demonstrated the ability to detect all 12 tested isolates of M. paspali , with no cross‐reactions observed when tested against 30 isolates of other fungal pathogens from turfgrass samples. The detection limit of the qPCR method was as low as 3.65 × 10 2 copies μL −1 of M. paspali genomic DNA, and the entire detection process could be completed within 1 h. The fluorescence signal was detectable in the leaf tissues of seashore paspalum without apparent disease symptoms as early as 24 h postinoculation with M. paspali . Moreover, the qPCR method successfully detected M. paspali in both asymptomatic and symptomatic turfgrass samples, including leaf, stem, root and rhizosphere soil, indicating that this assay can significantly enhance the detection of M. paspali . CONCLUSION The study developed a rapid real‐time qPCR assay for the detection of M. paspali causing SLP on seashore paspalum and in environmental samples, which has important implications for early warning and management of SLP. © 2024 Society of Chemical Industry.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
The assay detected all 12 tested Microdochium paspali isolates.
- Taxon
- seashore paspalum
Paspalum vaginatumt0 - Interactor
- M. paspaliMicrodochium paspalie0pathogen
- Outcome
- detect all 12 tested isolates of M. paspalie0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The qPCR assay demonstrated the ability to detect all 12 tested isolates of M. paspali”
t0“seashore paspalum”
Claim · model-written summary
The assay showed no cross-reactions with 30 other fungal isolates.
- Taxon
- seashore paspalumPaspalum vaginatumt0
- Outcome
- cross‐reactionse0
- Direction
- no cross‐reactionse0
- Value
- 30 isolatese0
- Comparator
- other fungal pathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“seashore paspalum”
e0“no cross‐reactions observed when tested against 30 isolates of other fungal pathogens from turfgrass samples.”
Claim · model-written summary
The qPCR detection limit was 3.65 × 10 2 copies μL −1 of pathogen DNA.
- Taxon
- seashore paspalumPaspalum vaginatumt0
- Interactor
- M. paspaliMicrodochium paspalie0pathogen
- Outcome
- detection limite0
- Value
- 3.65 × 10 2 copies μL −1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“seashore paspalum”
e0“The detection limit of the qPCR method was as low as 3.65 × 10 2 copies μL −1 of M. paspali genomic DNA”
Claim · model-written summary
The detection process took within one hour.
- Taxon
- seashore paspalumPaspalum vaginatumt0
- Outcome
- detection processe0
- Value
- 1 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“seashore paspalum”
e0“the entire detection process could be completed within 1 h.”
Claim · model-written summary
The fluorescence signal was detectable in asymptomatic leaves 24 hours after inoculation.
- Taxon
- seashore paspalumPaspalum vaginatume0
- Organ / stage
- leaf tissuese0
- Interactor
- M. paspaliMicrodochium paspalie0pathogen
- Outcome
- fluorescence signale0
- Value
- 24 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fluorescence signal was detectable in the leaf tissues of seashore paspalum without apparent disease symptoms as early as 24 h postinoculation with M. paspali .”
Claim · model-written summary
The assay detected Microdochium paspali in asymptomatic and symptomatic turfgrass samples.
- Taxon
- seashore paspalumPaspalum vaginatumt0
- Interactor
- M. paspaliMicrodochium paspalie0pathogen
- Outcome
- detected M. paspalie0
- Comparator
- asymptomatic and symptomatic turfgrass samplese0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“seashore paspalum”
e0“the qPCR method successfully detected M. paspali in both asymptomatic and symptomatic turfgrass samples, including leaf, stem, root and rhizosphere soil”