Abstract
ABSTRACT Grapevine Roditis leaf discolouration‐associated virus (GRLDaV) is from Badnavirus decoloratiovitis species, which is a member of the genus Badnavirus, associated with Roditis leaf discolouration disease. Although mealybug transmission of GRLDaV by Planococcus ficus has been demonstrated elsewhere, its role in the transmission has not been shown in different grapevine varieties, isolates, acquisition periods (AP) and inoculation periods (IP). Individiual P. ficus was collected from a vineyard in Adana, Turkey and used for insect culture establishment for the transmission studies. Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h. The virus was transmitted by P. ficus in all AP‐IP trials (100%, 60%, and 20% of recipient vines infected after 24, 48 h, and 72 h, respectively). GRLDaV was first detected in the recipient grapevines four weeks after transmission. The results showed that P. ficus transmitted GRLDaV in a semipersistent manner. All the virus infected vines in the experiment showed initial leaf symptoms of yellow vein banding and/or discolouration, followed by yellow and/or reddish discolouration of the leaves in late summer, as observed in GRLDaV‐infected vines in the field.
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Claims 8
Claim · model-written summary
Planococcus ficus transmitted GRLDaV to 100% of recipient vines after a 24-hour acquisition-inoculation period.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Treatment
- AP‐IPe0
- Interactor
- GRLDaVe0pathogen
- Second interactor
- P. ficus
Planococcus ficuse0pest - Outcome
- recipient vines infectede1
- Value
- 100%e1
- Qualifier
- after 24e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e1“The virus was transmitted by P. ficus in all AP‐IP trials (100%, 60%, and 20% of recipient vines infected after 24, 48 h, and 72 h, respectively)”
Claim · model-written summary
Planococcus ficus transmitted GRLDaV to 60% of recipient vines after a 48-hour acquisition-inoculation period.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Treatment
- AP‐IPe0
- Interactor
- GRLDaVe0pathogen
- Second interactor
- P. ficus
Planococcus ficuse0pest - Outcome
- recipient vines infectede1
- Value
- 60%e1
- Qualifier
- 48 he1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e1“The virus was transmitted by P. ficus in all AP‐IP trials (100%, 60%, and 20% of recipient vines infected after 24, 48 h, and 72 h, respectively)”
Claim · model-written summary
Planococcus ficus transmitted GRLDaV to 20% of recipient vines after a 72-hour acquisition-inoculation period.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Treatment
- AP‐IPe0
- Interactor
- GRLDaVe0pathogen
- Second interactor
- P. ficus
Planococcus ficuse0pest - Outcome
- recipient vines infectede1
- Value
- 20%e1
- Qualifier
- 72 he1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e1“The virus was transmitted by P. ficus in all AP‐IP trials (100%, 60%, and 20% of recipient vines infected after 24, 48 h, and 72 h, respectively)”
Claim · model-written summary
GRLDaV was first detected in the recipient grapevines four weeks after transmission.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Interactor
- GRLDaVe2pathogen
- Outcome
- first detected in the recipient grapevinese2
- Value
- four weekse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e2“GRLDaV was first detected in the recipient grapevines four weeks after transmission”
Claim · model-written summary
Planococcus ficus transmitted GRLDaV in a semipersistent manner.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Interactor
- GRLDaVe3pathogen
- Second interactor
- P. ficus
Planococcus ficuse3pest - Outcome
- transmittede3
- Qualifier
- in a semipersistent mannere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e3“The results showed that P. ficus transmitted GRLDaV in a semipersistent manner”
Claim · model-written summary
Infected vines first showed leaf symptoms of yellow vein banding and/or discolouration.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Organ / stage
- leafe4
- Interactor
- GRLDaVe0pathogen
- Outcome
- initial leaf symptomse4
- Qualifier
- yellow vein banding and/or discolouratione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e4“All the virus infected vines in the experiment showed initial leaf symptoms of yellow vein banding and/or discolouration”
Claim · model-written summary
Infected vines later showed yellow and/or reddish discolouration of the leaves in late summer.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Organ / stage
- leavese5
- Interactor
- GRLDaVe0pathogen
- Outcome
- yellow and/or reddish discolouratione5
- Qualifier
- in late summere5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e5“followed by yellow and/or reddish discolouration of the leaves in late summer”
Claim · model-written summary
Symptoms in the experiment were as observed in GRLDaV-infected vines in the field.
- Taxon
- Vitis vinifera cv. Yalova Incisi
Vitis viniferae0 - Interactor
- GRLDaVe6pathogen
- Outcome
- leaf symptomse4
- Qualifier
- as observed in GRLDaV‐infected vinese6
- Setting
- the fielde6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Virus transmission experiments to the Vitis vinifera cv. Yalova Incisi via P. ficus was carried out using a local GRLDaV isolate by applying AP‐IP for 24, 48 h or 72 h”
e4“All the virus infected vines in the experiment showed initial leaf symptoms of yellow vein banding and/or discolouration”
e6“as observed in GRLDaV‐infected vines in the field”