Abstract
Advanced multiplexed testing techniques should be designed and developed to ensure an accurate and reliable evaluation for unknown samples. In this study, an efficient platform coupled with the "Blue-Red-Purple" strategy based on recombinant polymerase amplification (RPA), CRISPR/Cas12a and lateral flow strip was established, which could realize the dual-target detection of CP4-EPSPS and Cry1Ab/Ac in genetically modified crops. The lateral flow immunoassay was developed using different colored microspheres to label the antibodies to realize the visualization of results and avoid cross-reactions. The proposed method exhibits high specificity, sensitivity and stability. The visual detection limits of standard plasmids and real samples reached 10 copies/μL and 0.5 %, respectively, which could be stored at 4 °C for 12 months with high detection ability. Moreover, the entire detection process could be completed within 50 min without any complex instruments or professional operators. These findings indicated that a sensitive, specific, rapid and accurate method was established for on-site detection of GM crops.
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Claims 5
Claim · model-written summary
The assay detected CP4-EPSPS and Cry1Ab/Ac in GM crops.
Not reportedValue
Verbatim
e0“dual-target detection of CP4-EPSPS and Cry1Ab/Ac in genetically modified crops”
t0““Blue-red-purple” multicolored lateral flow immunoassay for simultaneous detection of GM crops utilizing RPA and CRISPR/Cas12a”
Claim · model-written summary
Visual detection limit for standard plasmids reached 10 copies/μL.
Verbatim
e0“The visual detection limits of standard plasmids and real samples reached 10 copies/μL and 0.5 %, respectively”
t0““Blue-red-purple” multicolored lateral flow immunoassay for simultaneous detection of GM crops utilizing RPA and CRISPR/Cas12a”
Claim · model-written summary
Visual detection limit for real samples reached 0.5 %.
Verbatim
e0“The visual detection limits of standard plasmids and real samples reached 10 copies/μL and 0.5 %, respectively”
t0““Blue-red-purple” multicolored lateral flow immunoassay for simultaneous detection of GM crops utilizing RPA and CRISPR/Cas12a”
Claim · model-written summary
The assay retained detection ability after storage at 4 °C for 12 months.
Verbatim
e0“could be stored at 4 °C for 12 months with high detection ability”
t0““Blue-red-purple” multicolored lateral flow immunoassay for simultaneous detection of GM crops utilizing RPA and CRISPR/Cas12a”
Claim · model-written summary
The detection process could be completed within 50 minutes.
Verbatim
e0“entire detection process could be completed within 50 min”
t0““Blue-red-purple” multicolored lateral flow immunoassay for simultaneous detection of GM crops utilizing RPA and CRISPR/Cas12a”