Abstract
Background: Boron plays a crucial role in cell wall construction in plants and also facilitates the transfer of carbohydrates, which are essential for various physiological processes. The objective was to measure element concentrations in soil, water extracts and plant tissues (Zea mays) using Azomethine-H and ICP-MS within a controlled microplate assay. Methods: A microscopic plate repeat test was used to analyze the concentration of boron in soil extracts and plant tissue ash from local Zea mays leaves, considering the global trend in environmental sustainability. Tests aimed to modify the azomethine-H microscopic approach to eliminate pH and chemical interferences in soil samples and plant tissues. Due to their ability to ensure sample repeatability and quality control, micro spectrophotometers are ideal for high-throughput analysis. Result: The microplate test has enhanced boron measurement in soil and plant tissue samples. Our improvements to the microplate B assay have resulted in more accurate boron measurements in these samples. Microplate B testing is effective and suitable analysis in modern research and laboratories, using 40 times less chemical reagent per sample than traditional spectrophotometry. Additionally, microplates enable simultaneous analysis and calibration of samples. The modified plate test significantly improves boron measurement in soil and plant tissue samples, using 40 times fewer reagents than traditional spectrophotometry. This method is cost-effective, ideal research, commercial analysis and making it a valuable tool for modern laboratories.
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Claims 3
Claim · model-written summary
The microplate assay improved boron measurement accuracy in plant tissue and soil samples.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Our improvements to the microplate B assay have resulted in more accurate boron measurements in these samples.”
t0“Zea mays”
Claim · model-written summary
The assay used 40 times less chemical reagent per sample than traditional spectrophotometry.
- Outcome
- chemical reagent per samplee0
- Direction
- lesse0
- Value
- 40 times lesse0
- Comparator
- traditional spectrophotometrye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“using 40 times less chemical reagent per sample than traditional spectrophotometry.”
t0“Zea mays”
Claim · model-written summary
Microplates enabled simultaneous sample analysis and calibration.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“microplates enable simultaneous analysis and calibration of samples.”
t0“Zea mays”