Paper · 2024 · Agricultural Science Digest - A Research Journal

Using Microplates to Test Boron in Zea mays Leaf Plant and the Surrounding Soil

Estabraq Mohammed Ati; Rana Fadhil Abbas; Abrar Thamer Al-Safaar; et al.

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claims
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verbatim quotes
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citations
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Open access · cc-by

Abstract

Background: Boron plays a crucial role in cell wall construction in plants and also facilitates the transfer of carbohydrates, which are essential for various physiological processes. The objective was to measure element concentrations in soil, water extracts and plant tissues (Zea mays) using Azomethine-H and ICP-MS within a controlled microplate assay. Methods: A microscopic plate repeat test was used to analyze the concentration of boron in soil extracts and plant tissue ash from local Zea mays leaves, considering the global trend in environmental sustainability. Tests aimed to modify the azomethine-H microscopic approach to eliminate pH and chemical interferences in soil samples and plant tissues. Due to their ability to ensure sample repeatability and quality control, micro spectrophotometers are ideal for high-throughput analysis. Result: The microplate test has enhanced boron measurement in soil and plant tissue samples. Our improvements to the microplate B assay have resulted in more accurate boron measurements in these samples. Microplate B testing is effective and suitable analysis in modern research and laboratories, using 40 times less chemical reagent per sample than traditional spectrophotometry. Additionally, microplates enable simultaneous analysis and calibration of samples. The modified plate test significantly improves boron measurement in soil and plant tissue samples, using 40 times fewer reagents than traditional spectrophotometry. This method is cost-effective, ideal research, commercial analysis and making it a valuable tool for modern laboratories.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 3

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The microplate assay improved boron measurement accuracy in plant tissue and soil samples.

Taxon
Zea maysZea mayst0
Outcome
boron measurementse0
Comparator
more accuratee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Our improvements to the microplate B assay have resulted in more accurate boron measurements in these samples.”

✓ located verbatim in the source · abstract, characters 942–1052 · show in text ↑
t0

“Zea mays”

✓ located verbatim in the source · title, characters 35–43 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The assay used 40 times less chemical reagent per sample than traditional spectrophotometry.

Taxon
Zea maysZea mayst0
Outcome
chemical reagent per samplee0
Direction
lesse0
Value
40 times lesse0
Comparator
traditional spectrophotometrye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“using 40 times less chemical reagent per sample than traditional spectrophotometry.”

✓ located verbatim in the source · abstract, characters 1146–1229 · show in text ↑
t0

“Zea mays”

✓ located verbatim in the source · title, characters 35–43 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Microplates enabled simultaneous sample analysis and calibration.

Taxon
Zea maysZea mayst0
Outcome
simultaneous analysis and calibration of samplese0
Value
simultaneouse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“microplates enable simultaneous analysis and calibration of samples.”

✓ located verbatim in the source · abstract, characters 1244–1312 · show in text ↑
t0

“Zea mays”

✓ located verbatim in the source · title, characters 35–43 · show in text ↑