Abstract
Rice seed-borne diseases caused by the bacterial pathogens Burkholderia glumae and B. plantarii pose a major threat to rice production worldwide. To manage these diseases in a sustainable manner, a biocontrol strategy is crucial. In this study, we showed that B. gladioli NB6 (NB6), a nonpathogenic bacterium, strongly protects rice from infection caused by the above-mentioned pathogens. NB6 was isolated from the indica rice cultivar Nona Bokra seedlings, which possesses genetic resistance to B. glumae. We discovered that cell suspensions of NB6 and its culture filtrate suppressed the disease symptoms caused by B. glumae and B. plantarii in rice seedlings, which indicated that NB6 secretes a plant-protective substance extracellularly. Through purification and mass spectrometry analysis of the culture filtrate, combined with transmission electron microscopy and mutant analysis, the substance was identified as a tailocin and named BglaTNB6. Tailocins are bacteriotoxic multiprotein structures morphologically similar to headless phage tails. BglaTNB6 exhibited antibacterial activity against several Burkholderia species, including B. glumae, B. plantarii, and B. gladioli, suggesting it can prevent pathogen infection. Interestingly, BglaTNB6 greatly contributed only to the biocontrol activity of NB6 cell suspensions against B. plantarii, and not against B. glumae. BglaTNB6 was shown to be encoded by a prophage locus lacking genes for phage head proteins, and a B. gladioli strain with the coded BglaTNB6-like locus equipped with phage head proteins failed to prevent rice seedlings from being infected with B. plantarii. These results suggested that BglaTNB6 may enhance the competitiveness of NB6 against a specific range of bacteria. Our study also highlights the potential of tailocin-producing endophytes for managing crop bacterial diseases.
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Claims 11
Claim · model-written summary
NB6 cell suspensions suppressed B. glumae disease symptoms in rice seedlings.
- Taxon
- rice
Oryza sativae0 - Treatment
- cell suspensions of NB6e0
- Interactor
- B. glumae
Burkholderia glumaee0pathogen - Second interactor
- B. gladioli NB6
Burkholderia gladiolie1biocontrol_agent - Outcome
- disease symptoms caused by B. glumaee0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell suspensions of NB6 and its culture filtrate suppressed the disease symptoms caused by B. glumae and B. plantarii in rice seedlings”
e1“B. gladioli NB6 (NB6), a nonpathogenic bacterium”
Claim · model-written summary
NB6 culture filtrate suppressed B. glumae disease symptoms in rice seedlings.
- Taxon
- riceOryza sativae0
- Treatment
- its culture filtratee0
- Interactor
- B. glumaeBurkholderia glumaee0pathogen
- Second interactor
- B. gladioli NB6Burkholderia gladiolie1biocontrol_agent
- Outcome
- disease symptoms caused by B. glumaee0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell suspensions of NB6 and its culture filtrate suppressed the disease symptoms caused by B. glumae and B. plantarii in rice seedlings”
e1“B. gladioli NB6 (NB6), a nonpathogenic bacterium”
Claim · model-written summary
NB6 cell suspensions suppressed B. plantarii disease symptoms in rice seedlings.
- Taxon
- riceOryza sativae0
- Treatment
- cell suspensions of NB6e0
- Interactor
- B. plantariiBurkholderia plantariie0pathogen
- Second interactor
- B. gladioli NB6Burkholderia gladiolie1biocontrol_agent
- Outcome
- disease symptoms caused by B. glumae and B. plantariie0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell suspensions of NB6 and its culture filtrate suppressed the disease symptoms caused by B. glumae and B. plantarii in rice seedlings”
e1“B. gladioli NB6 (NB6), a nonpathogenic bacterium”
Claim · model-written summary
NB6 culture filtrate suppressed B. plantarii disease symptoms in rice seedlings.
- Taxon
- riceOryza sativae0
- Treatment
- its culture filtratee0
- Interactor
- B. plantariiBurkholderia plantariie0pathogen
- Second interactor
- B. gladioli NB6Burkholderia gladiolie1biocontrol_agent
- Outcome
- disease symptoms caused by B. glumae and B. plantariie0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell suspensions of NB6 and its culture filtrate suppressed the disease symptoms caused by B. glumae and B. plantarii in rice seedlings”
e1“B. gladioli NB6 (NB6), a nonpathogenic bacterium”
Claim · model-written summary
The secreted protective substance was identified as the tailocin BglaTNB6.
- Taxon
- riceOryza sativae1
- Outcome
- identified as a tailocin and named BglaTNB6e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the substance was identified as a tailocin and named BglaTNB6”
e1“failed to prevent rice seedlings”
Claim · model-written summary
BglaTNB6 had antibacterial activity against B. glumae.
- Taxon
- riceOryza sativae1
- Interactor
- B. glumaeBurkholderia glumaee0pathogen
- Outcome
- antibacterial activity against several Burkholderia speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BglaTNB6 exhibited antibacterial activity against several Burkholderia species, including B. glumae, B. plantarii, and B. gladioli”
e1“failed to prevent rice seedlings”
Claim · model-written summary
BglaTNB6 had antibacterial activity against B. plantarii.
- Taxon
- riceOryza sativae1
- Interactor
- B. plantariiBurkholderia plantariie0pathogen
- Outcome
- antibacterial activity against several Burkholderia speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BglaTNB6 exhibited antibacterial activity against several Burkholderia species, including B. glumae, B. plantarii, and B. gladioli”
e1“failed to prevent rice seedlings”
Claim · model-written summary
BglaTNB6 had antibacterial activity against B. gladioli.
- Taxon
- riceOryza sativae1
- Interactor
- B. gladioliBurkholderia gladiolie0other
- Outcome
- antibacterial activity against several Burkholderia speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BglaTNB6 exhibited antibacterial activity against several Burkholderia species, including B. glumae, B. plantarii, and B. gladioli”
e1“failed to prevent rice seedlings”
Claim · model-written summary
BglaTNB6 contributed to NB6 biocontrol against B. plantarii.
- Taxon
- riceOryza sativae1
- Interactor
- B. plantariiBurkholderia plantariie0pathogen
- Second interactor
- B. gladioli NB6Burkholderia gladiolie2biocontrol_agent
- Outcome
- biocontrol activity of NB6 cell suspensions against B. plantariie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BglaTNB6 greatly contributed only to the biocontrol activity of NB6 cell suspensions against B. plantarii”
e1“failed to prevent rice seedlings”
e2“B. gladioli NB6 (NB6), a nonpathogenic bacterium”
Claim · model-written summary
BglaTNB6 did not contribute to NB6 biocontrol against B. glumae.
- Taxon
- riceOryza sativae2
- Interactor
- B. glumaeBurkholderia glumaee0pathogen
- Outcome
- biocontrol activity of NB6 cell suspensionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and not against B. glumae”
e1“BglaTNB6 greatly contributed only to the biocontrol activity of NB6 cell suspensions against B. plantarii”
e2“failed to prevent rice seedlings”
Claim · model-written summary
A strain with a BglaTNB6-like locus carrying phage head proteins failed to prevent B. plantarii infection of rice seedlings.
- Taxon
- riceOryza sativae0
- Treatment
- a B. gladioli strain with the coded BglaTNB6-like locus equipped with phage head proteinse0
- Interactor
- B. plantariiBurkholderia plantariie0pathogen
- Second interactor
- B. gladioliBurkholderia gladiolie0biocontrol_agent
- Outcome
- rice seedlings from being infected with B. plantariie0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a B. gladioli strain with the coded BglaTNB6-like locus equipped with phage head proteins failed to prevent rice seedlings from being infected with B. plantarii”
The same questions in other papers
Burkholderia gladioli · Burkholderia plantarii · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
pathogen product · Burkholderia glumae · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.