Abstract
This study aimed to investigate the existence of wheat seed gall nematode Anguina tritici in the silos and some wheat fileds in Duhok province / Kurdistan Region - Iraq.,and diagnose four new isolates of this nematode on wheat and one isolate on barley collected from different loactions in Iraq.The results indiacted that wheat impurities samples were infested with seed galls by 66 % as the highest percentager in silo of Faidia during 2020, while the lowest (6%) recoded in the silo of Zakho during the same year.The highest disease incidence was recorded in the wheat fields of Akre by 34.6%,while the lowest (2%) in the wheat fields of Semel. Molecular identification results revealed that the bands of amplified DNA products of all nematode isolates were visualized by agarose gel electrophoresis in the same position ("~" up to 750 bp).Sequencing of the partial gene 5.8S rRNA gene confirmed that all nematode isolates belong to seed gall nematode A.tritici, also a description of their accession number by blast program showed the same percentage identitiy (100 %), and their comparision by a DNA Dot Plot emphasized that they are genetically similar. Results of phylogenetic tree analysis showed grouping of nematode isolates with each other and with the other two isolates of the same species from Iraq,whilst nucleotide variations increased with other nematode species of the same genus,and increased more with other nematode species of the same family (Anguinidae).
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Claims 9
Claim · model-written summary
Wheat impurities had 66 % seed gall infestation in Faidia silo in 2020, the highest reported.
- Taxon
- wheat
Triticum aestivume0 - Outcome
- seed gallse0
- Direction
- higheste0
- Value
- 66 %e0
- Setting
- silo of Faidiae0
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wheat impurities samples were infested with seed galls by 66 % as the highest percentager in silo of Faidia during 2020”
Claim · model-written summary
Wheat impurities had 6% seed gall infestation in Zakho silo, the lowest reported.
Not reportedTaxon
Verbatim
e0“wheat impurities samples were infested with seed galls by 66 % as the highest percentager in silo of Faidia during 2020, while the lowest (6%) recoded in the silo of Zakho during the same year”
Claim · model-written summary
Wheat fields in Akre had the highest disease incidence at 34.6%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest disease incidence was recorded in the wheat fields of Akre by 34.6%”
Claim · model-written summary
Wheat fields in Semel had the lowest disease incidence at 2%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest disease incidence was recorded in the wheat fields of Akre by 34.6%,while the lowest (2%) in the wheat fields of Semel”
Claim · model-written summary
All nematode isolates produced DNA bands at approximately 750 bp.
Not reportedTaxon
Verbatim
e0“bands of amplified DNA products of all nematode isolates were visualized by agarose gel electrophoresis in the same position ("~" up to 750 bp)”
Claim · model-written summary
Partial 5.8S rRNA gene sequences identified all isolates as A.tritici with 100 % identity.
- Interactor
- A.tritici
.jpg?width=60)
Anguina triticie0pathogen - Gene
- 5.8S rRNA genee0
- Outcome
- same percentage identitiye0
- Value
- 100 %e0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Sequencing of the partial gene 5.8S rRNA gene confirmed that all nematode isolates belong to seed gall nematode A.tritici, also a description of their accession number by blast program showed the same percentage identitiy (100 %)”
Claim · model-written summary
DNA Dot Plot comparison found the nematode isolates genetically similar.
- Outcome
- genetically similare0
Not reportedTaxon
Verbatim
e0“their comparision by a DNA Dot Plot emphasized that they are genetically similar”
Claim · model-written summary
Phylogenetic analysis grouped these isolates with each other and two other Iraqi isolates.
- Outcome
- grouping of nematode isolates with each other and with the other two isolates of the same speciese0
- Setting
- Iraqe0
Not reportedTaxon
Verbatim
e0“grouping of nematode isolates with each other and with the other two isolates of the same species from Iraq”
Claim · model-written summary
Nucleotide variation increased relative to other species in the genus and increased further relative to other Anguinidae species.
- Outcome
- nucleotide variationse0
- Direction
- increasede0
- Comparator
- other nematode species of the same genuse0
Not reportedTaxon
Verbatim
e0“nucleotide variations increased with other nematode species of the same genus,and increased more with other nematode species of the same family (Anguinidae)”