Abstract
In vitro root cultivation techniques based on modified root systems are often used in studies on Arbuscular Mycorrhizal Fungi (AMF). It is a simplified but powerful tool to investigate AMF root colonization and development of the extraradical mycelium. The aim of this study was to establish and characterize the in vitro culture of a Cuban strain of Rhizophagus irregularis (INCAM 11) by using transformed chicory roots. For that, superficially disinfected propagules of R. irregularis were co-culture with the hairy transformed chicory roots on Modified Strullu and Romand (MSR) medium during five months. Spore germination was observed 3-5 days after surface disinfection. The first contact between AMF hyphae and roots occurred 1 - 3 days after germination and a significant production of extensive extraradical mycelium was observed. New spore formation started within 21 - 25 days. After 5 months, 2000 spores could be observed per plate which were able to germinate, colonize, establish and reproduce again spores when associated to young transformed roots of chicory. The most frequent associated microorganism to the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp.
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Claims 6
Claim · model-written summary
Spore germination of R. irregularis INCAM 11 was observed 3-5 days after surface disinfection.
- Taxon
- Rhizophagus irregularis
Rhizophagus irregularise2 - Outcome
- Spore germinatione1
- Value
- 3-5 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Spore germination was observed 3-5 days after surface disinfection”
e2“Rhizophagus irregularis”
Claim · model-written summary
The first contact between AMF hyphae and roots occurred 1-3 days after germination.
- Taxon
- Rhizophagus irregularisRhizophagus irregularise2
- Outcome
- first contact between AMF hyphae and rootse1
- Value
- 1 - 3 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The first contact between AMF hyphae and roots occurred 1 - 3 days after germination”
e2“Rhizophagus irregularis”
Claim · model-written summary
A significant production of extensive extraradical mycelium was observed.
- Taxon
- Rhizophagus irregularisRhizophagus irregularise2
- Outcome
- production of extensive extraradical myceliume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a significant production of extensive extraradical mycelium was observed”
e2“Rhizophagus irregularis”
Claim · model-written summary
New spore formation started within 21-25 days.
- Taxon
- Rhizophagus irregularisRhizophagus irregularise2
- Outcome
- New spore formatione1
- Value
- 21 - 25 dayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“New spore formation started within 21 - 25 days”
e2“Rhizophagus irregularis”
Claim · model-written summary
After 5 months about 2000 spores per plate were observed, able to germinate, colonize, establish and reproduce spores again on young transformed chicory roots.
- Taxon
- Rhizophagus irregularisRhizophagus irregularise2
- Outcome
- sporese1
- Value
- 2000e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“After 5 months, 2000 spores could be observed per plate which were able to germinate, colonize, establish and reproduce again spores when associated to young transformed roots of chicory”
e2“Rhizophagus irregularis”
Claim · model-written summary
The most frequent microorganism associated with the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp.
- Taxon
- Rhizophagus irregularisRhizophagus irregularise2
- Interactor
- Paenibacillus sp.
Paenibacilluse1other - Outcome
- most frequent associated microorganisme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The most frequent associated microorganism to the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp.”
e2“Rhizophagus irregularis”