Abstract
Chrysanthemum morifolium , a kind of medicine and food homology product, is threatened by plant pathogens. It is crucial to establish a sensitive and accurate method for diagnosing its pathogens. Therefore, the internal transcribed spacer regions of the ribosomal DNA (rDNA) sequences of three common pathogens in C. morifolium ( Chaetomium globosum , Fusarium incarnatum , and Alternaria alternata ) were compared, and the three pairs of specific primers were achieved. A two‐step nested multiplex PCR system which consists of two sequential amplification steps was successfully developed to detect those three pathogens in C. morifolium . This method was sensitive due to its low detection limits, which were 10 pg/μL, 1 pg/μL, and 10 fg/μL for C. globosum , F. incarnatum , and A. alternata , respectively. The entire detection process of this method only took 5–6 h, demonstrating its high efficiency. The nested multiplex PCR system could be successfully applied to detect actual samples, and a 90% detection rate for those three pathogens in selected C. morifolium was achieved. This suggests that our assay could be an operator‐friendly tool for diagnosing C. morifolium diseases.
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Claims 6
Claim · model-written summary
Specific primers were obtained for three chrysanthemum pathogens.
- Taxon
- Chrysanthemum morifolium
Chrysanthemum ×morifoliumt0 - Outcome
- specific primerse0
- Value
- three pairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three pairs of specific primers were achieved.”
t0“Chrysanthemum morifolium”
Claim · model-written summary
Nested multiplex PCR detected Chaetomium globosum in chrysanthemum at 10 pg/μL.
- Taxon
- Chrysanthemum morifoliumChrysanthemum ×morifoliumt0
- Interactor
- C. globosum
Chaetomium globosume0pathogen - Outcome
- detection limitse0
- Value
- 10 pg/μLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method was sensitive due to its low detection limits, which were 10 pg/μL, 1 pg/μL, and 10 fg/μL for C. globosum , F. incarnatum , and A. alternata , respectively.”
t0“Chrysanthemum morifolium”
Claim · model-written summary
Nested multiplex PCR detected Fusarium incarnatum in chrysanthemum at 1 pg/μL.
- Taxon
- Chrysanthemum morifoliumChrysanthemum ×morifoliumt0
- Interactor
- F. incarnatumFusarium incarnatume0pathogen
- Outcome
- detection limitse0
- Value
- 1 pg/μLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method was sensitive due to its low detection limits, which were 10 pg/μL, 1 pg/μL, and 10 fg/μL for C. globosum , F. incarnatum , and A. alternata , respectively.”
t0“Chrysanthemum morifolium”
Claim · model-written summary
Nested multiplex PCR detected Alternaria alternata in chrysanthemum at 10 fg/μL.
- Taxon
- Chrysanthemum morifoliumChrysanthemum ×morifoliumt0
- Interactor
- A. alternata
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Alternaria alternatae0pathogen - Outcome
- detection limitse0
- Value
- 10 fg/μLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“This method was sensitive due to its low detection limits, which were 10 pg/μL, 1 pg/μL, and 10 fg/μL for C. globosum , F. incarnatum , and A. alternata , respectively.”
t0“Chrysanthemum morifolium”
Claim · model-written summary
The PCR detection process took 5–6 h.
- Taxon
- Chrysanthemum morifoliumChrysanthemum ×morifoliumt0
- Outcome
- detection processe0
- Value
- 5–6 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The entire detection process of this method only took 5–6 h, demonstrating its high efficiency.”
t0“Chrysanthemum morifolium”
Claim · model-written summary
The PCR system detected pathogens in actual chrysanthemum samples at a 90% rate.
- Taxon
- C. morifoliumChrysanthemum ×morifoliume0
- Outcome
- detection ratee0
- Value
- 90%e0
- Qualifier
- actual samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nested multiplex PCR system could be successfully applied to detect actual samples, and a 90% detection rate for those three pathogens in selected C. morifolium was achieved.”