Abstract
DNA methylation is one of the earliest and most extensively studied epigenetic regulatory mechanisms. The ROS1 (Repressor of Silencing 1) gene was first discovered in Arabidopsis thaliana, and it is a DNA demethylase that can remove 5-methylcytosine from DNA, thereby affecting DNA methylation levels and gene expression. Objectives: The objective of this study was to investigate the role of ROS1 in the development and maturation of Ziziphus jujuba cv. “Dongzao” fruit. Methods: We cloned the ROS1 gene and conducted bioinformatics and expression characteristics analyses on it. Results: Three ROS1 genes, named ZjROS1-1~3, was identified, and each member protein was localized in the nucleus, cytoskeleton, chloroplast, and vacuole. The promoter contained cis-elements such as light response, plant hormone signal transduction, and stress response cis-elements, and it interacted with many proteins such as CMT, MET, and ZDP. The results of the real-time fluorescence quantitative PCR show that ZjROS1 has specific expression patterns in different tissues of Z. jujuba cv. Dongzao, and the expression of ZjROS1-2 in flowers and fruits is high. At the same time, CRISPR/Cas9 technology was used to construct a gene-editing vector for ZjROS1, which provided a basis for the subsequent genetic transformation. Conclusions: In this study, the biological function of ZjROS1 was clarified and a gene-editing vector was constructed, which provided a theoretical basis for the regulation mechanism of demethylase ZjROS1 in the fruit ripening and development of Z. jujuba cv. Dongzao.
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Claims 7
Claim · model-written summary
Three ZjROS1 genes (ZjROS1-1 to ZjROS1-3) were identified in jujube.
- Taxon
- Ziziphus jujuba cv. Dongzao
Ziziphus jujubat0 - Gene
- ZjROS1-1~3e1
- Outcome
- ROS1 genese1
- Value
- Threee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ziziphus jujuba cv. Dongzao DNA Demethylase ZjROS1 Gene Family”
e1“Three ROS1 genes, named ZjROS1-1~3, was identified”
Claim · model-written summary
Each ZjROS1 member protein was localized in the nucleus, cytoskeleton, chloroplast and vacuole.
- Taxon
- Ziziphus jujuba cv. DongzaoZiziphus jujubat0
- Outcome
- member protein was localizede2
- Qualifier
- nucleus, cytoskeleton, chloroplast, and vacuolee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ziziphus jujuba cv. Dongzao DNA Demethylase ZjROS1 Gene Family”
e2“each member protein was localized in the nucleus, cytoskeleton, chloroplast, and vacuole”
Claim · model-written summary
ZjROS1 shows specific expression patterns across different jujube tissues.
- Taxon
- Z. jujuba cv. DongzaoZiziphus jujubae3
- Organ / stage
- different tissuese3
- Gene
- ZjROS1e3
- Outcome
- expression patternse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ziziphus jujuba cv. Dongzao DNA Demethylase ZjROS1 Gene Family”
e3“ZjROS1 has specific expression patterns in different tissues of Z. jujuba cv. Dongzao”
Claim · model-written summary
ZjROS1-2 expression is high in jujube flowers and fruits.
- Taxon
- Ziziphus jujuba cv. DongzaoZiziphus jujubat0
- Organ / stage
- flowers and fruitse4
- Gene
- ZjROS1-2e4
- Outcome
- expressione4
- Direction
- highe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ziziphus jujuba cv. Dongzao DNA Demethylase ZjROS1 Gene Family”
e4“the expression of ZjROS1-2 in flowers and fruits is high”
Claim · model-written summary
A CRISPR/Cas9 gene-editing vector for ZjROS1 was constructed.
- Taxon
- Ziziphus jujuba cv. DongzaoZiziphus jujubat0
- Gene
- ZjROS1e5
- Outcome
- gene-editing vectore5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ziziphus jujuba cv. Dongzao DNA Demethylase ZjROS1 Gene Family”
e5“CRISPR/Cas9 technology was used to construct a gene-editing vector for ZjROS1”
Claim · model-written summary
The ZjROS1 promoter contains cis-elements for light response, plant hormone signal transduction and stress response.
- Taxon
- Ziziphus jujuba cv. DongzaoZiziphus jujubat0
- Outcome
- cis-elementse6
- Qualifier
- light response, plant hormone signal transduction, and stress responsee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ziziphus jujuba cv. Dongzao DNA Demethylase ZjROS1 Gene Family”
e6“The promoter contained cis-elements such as light response, plant hormone signal transduction, and stress response cis-elements”
Claim · model-written summary
ZjROS1 interacts with many proteins, including CMT, MET and ZDP.
- Taxon
- Ziziphus jujuba cv. DongzaoZiziphus jujubat0
- Gene
- CMT, MET, and ZDPe7
- Outcome
- interacted with many proteinse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ziziphus jujuba cv. Dongzao DNA Demethylase ZjROS1 Gene Family”
e7“it interacted with many proteins such as CMT, MET, and ZDP”