Abstract
This research investigated optimization of sterilization techniques and cytokinin concentrations to improve the in vitro propagation of turmeric (Curcuma longa). Given the limitations of conventional cultivation methods, such as susceptibility to diseases, slow growth and more area requirements, tissue culture techniques provide a more efficient alternative for controlling propagation. The study conducted in the Tissue Culture Laboratory’ Faculty of Environmental Agricultural Sciences, Arish University, Egypt. During the initial establishment phase, rhizome buds were sterilized using commercial Clorox at concentrations of 10%, 15%, and 20% for varying periods (5, 10 and 15 min.). The most successful sterilization approach was achieved with 20% Clorox applied for 10 minutes, resulting in a 90% survival rate and minimal contamination. For shoot multiplication, different cytokinins such as thidiazuron (TDZ), benzyl adenine benzyl adenine (BA), and Kinetin were evaluated separately at different concentrations. The best shoot proliferation was observed with 1.0 mg l⁻¹ TDZ. In the rooting phase, growth media fortified with Naphthalene Acetic Acid (NAA) at concentrations ranging from 0.5 to 2.0 mg l⁻¹ significantly influenced the root growth’ Moreover, rooting medium supplemented with 1.5 mg l⁻¹ NAA produced the longest roots. For acclimatization, a mixture of peat moss, vermiculite, and washed sand in a 1:1:1 ratio (V/V/V) was used, resulting in an 80% survival rate for the plants. The findings highlight the importance of optimized sterilization methods and growth regulator concentrations in improving the tissue culture propagation of turmeric, offering promising potential for the large-scale production of this medicinally important plant.
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Claims 6
Claim · model-written summary
Twenty percent Clorox for 10 minutes gave 90% turmeric explant survival with minimal contamination.
- Taxon
- TURMERIC
_W_IMG_2440.jpg?width=60)
Curcuma longat0 - Treatment
- 20% Clorox applied for 10 minutese0
- Outcome
- survival ratee0
- Direction
- most successfule0
- Value
- 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_W_IMG_2440.jpg?width=500)
Verbatim
e0“The most successful sterilization approach was achieved with 20% Clorox applied for 10 minutes, resulting in a 90% survival rate and minimal contamination”
t0“TURMERIC (Curcuma longa)”
Claim · model-written summary
Twenty percent Clorox for 10 minutes produced minimal contamination.
- Taxon
- TURMERICCurcuma longat0
- Treatment
- 20% Clorox applied for 10 minutese0
- Outcome
- contaminatione0
- Direction
- minimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most successful sterilization approach was achieved with 20% Clorox applied for 10 minutes, resulting in a 90% survival rate and minimal contamination”
t0“TURMERIC (Curcuma longa)”
Claim · model-written summary
TDZ at 1.0 mg l⁻¹ gave the best shoot proliferation.
- Taxon
- TURMERICCurcuma longat0
- Treatment
- 1.0 mg l⁻¹ TDZe0
- Outcome
- shoot proliferatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best shoot proliferation was observed with 1.0 mg l⁻¹ TDZ”
t0“TURMERIC (Curcuma longa)”
Claim · model-written summary
NAA in rooting medium significantly influenced root growth.
- Taxon
- TURMERICCurcuma longat0
- Treatment
- Naphthalene Acetic Acid (NAA) at concentrations ranging from 0.5 to 2.0 mg l⁻¹e0
- Outcome
- root growthe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“growth media fortified with Naphthalene Acetic Acid (NAA) at concentrations ranging from 0.5 to 2.0 mg l⁻¹ significantly influenced the root growth”
t0“TURMERIC (Curcuma longa)”
Claim · model-written summary
Rooting medium with 1.5 mg l⁻¹ NAA produced the longest roots.
- Taxon
- TURMERICCurcuma longat0
- Treatment
- 1.5 mg l⁻¹ NAAe0
- Outcome
- rootse0
- Direction
- longeste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooting medium supplemented with 1.5 mg l⁻¹ NAA produced the longest roots”
t0“TURMERIC (Curcuma longa)”
Claim · model-written summary
A 1:1:1 peat moss, vermiculite and sand acclimatization mix yielded 80% plant survival.
- Taxon
- TURMERICCurcuma longat0
- Treatment
- a mixture of peat moss, vermiculite, and washed sand in a 1:1:1 ratio (V/V/V)e0
- Outcome
- survival ratee0
- Value
- 80%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a mixture of peat moss, vermiculite, and washed sand in a 1:1:1 ratio (V/V/V) was used, resulting in an 80% survival rate for the plants”
t0“TURMERIC (Curcuma longa)”
The same questions in other papers
Thidiazuron · growth · Curcuma longa (turmeric)Results agree
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- positive
1-naphthaleneacetic acid · growth · Curcuma longa (turmeric)Results agree
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- positive
- positive
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.