Paper · 2024 · Chagyo Kenkyu Hokoku (Tea Research Journal)

Isolation of Exobasidium vexans from the Lesions of Blister Blight of Tea and the Prevalence of Anamorphic Structures in Culture

Hideyuki Nagao; Takamichi Orihara; Taiga Kasuya

6
claims
12
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

Tissue isolation was attempted with lesions of blister blight of tea to obtain colonies of Exobasidium vexans. After the ordinary surface sterilization method, colonies of E. vexans emerged from 13 leaf pieces in 180 leaf pieces examined (8.1%) collected in the Ashigara area, Kanagawa, Japan, on calcium–amended PDA. Fungal contamination occurred on 56.9% of the leaf pieces. Colony morphology of the tissue isolates was the same as that obtained from a mass basidiospore isolation. Colonies were composed of pseudohyphae and blastospores and gradually transformed to chlamydospore-like bodies (CLB) after 5 months. Germinability of CLB was tested by spreading a colony suspension on calcium–amended PDA. After 10 days incubation, CLB germinated and produced blastospores. Four germination types of CLB were categorized.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Colonies of E. vexans emerged from 13 of 180 tea lesion leaf pieces examined (8.1%) by tissue isolation.

Taxon
Teat0
Interactor
E. vexansExobasidium vexanse0pathogen
Outcome
emerged from 13 leaf pieces in 180 leaf pieces examinede0
Value
8.1%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Exobasidium vexans
Exobasidium vexans · InteractorJürgen Kranz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“colonies of E. vexans emerged from 13 leaf pieces in 180 leaf pieces examined (8.1%)”

✓ located verbatim in the source · abstract, characters 160–244 · show in text ↑
t0

“Tea”

✓ located verbatim in the source · title, characters 70–73 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fungal contamination occurred on 56.9% of the leaf pieces.

Taxon
Teat0
Outcome
Fungal contaminatione0
Value
56.9%e0

Verbatim

e0

“Fungal contamination occurred on 56.9% of the leaf pieces”

✓ located verbatim in the source · abstract, characters 318–375 · show in text ↑
t0

“Tea”

✓ located verbatim in the source · title, characters 70–73 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Colony morphology of the tissue isolates was the same as that of a mass basidiospore isolation.

Taxon
Exobasidium vexansExobasidium vexanst0
Outcome
Colony morphology of the tissue isolatese0
Comparator
mass basidiospore isolatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Exobasidium vexans
Exobasidium vexans · TaxonJürgen Kranz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Colony morphology of the tissue isolates was the same as that obtained from a mass basidiospore isolation”

✓ located verbatim in the source · abstract, characters 377–482 · show in text ↑
t0

“Exobasidium vexans”

✓ located verbatim in the source · title, characters 13–31 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Colonies were composed of pseudohyphae and blastospores and gradually transformed to chlamydospore-like bodies after 5 months.

Taxon
Exobasidium vexansExobasidium vexanst0
Outcome
composed of pseudohyphae and blastospores and gradually transformed to chlamydospore-like bodiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Exobasidium vexans
Exobasidium vexans · TaxonJürgen Kranz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Colonies were composed of pseudohyphae and blastospores and gradually transformed to chlamydospore-like bodies (CLB) after 5 months”

✓ located verbatim in the source · abstract, characters 484–615 · show in text ↑
t0

“Exobasidium vexans”

✓ located verbatim in the source · title, characters 13–31 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

After 10 days of incubation the chlamydospore-like bodies germinated and produced blastospores.

Taxon
Exobasidium vexansExobasidium vexanst0
Outcome
CLB germinated and produced blastosporese0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Exobasidium vexans
Exobasidium vexans · TaxonJürgen Kranz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“After 10 days incubation, CLB germinated and produced blastospores”

✓ located verbatim in the source · abstract, characters 706–772 · show in text ↑
t0

“Exobasidium vexans”

✓ located verbatim in the source · title, characters 13–31 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Four germination types of the chlamydospore-like bodies were categorized.

Taxon
Exobasidium vexansExobasidium vexanst0
Outcome
Four germination types of CLBe0
Value
Foure0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Exobasidium vexans
Exobasidium vexans · TaxonJürgen Kranz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Four germination types of CLB were categorized”

✓ located verbatim in the source · abstract, characters 774–820 · show in text ↑
t0

“Exobasidium vexans”

✓ located verbatim in the source · title, characters 13–31 · show in text ↑