Abstract
This study aimed to investigate the endophytic microbial populations associated with wild and domesticated grapevines using both culture-based and culture-independent methods. Through culture-based methods, 148 endophytes were identified. The dominant fungal species included Aureobasidium pullulans, Alternaria alternata, and Cladosporium allicinum, while predominant bacterial species were Ralstonia pikettii, Nocardia niigatensis, and Sphingomonas echinoides. Culture-independent methods employed metagenomic techniques to explore microbial biodiversity, focusing on targeted amplification of bacterial 16S rRNA as well as fungal ITS and 26S rRNA gene regions. The main bacterial species identified included Halomonas sp., Sphingomonas sp. and Massilia sp., whereas the fungal population was dominated by Cladosporium sp., Malassezia sp. and Mucor sp. The findings revealed that vineyard management practices did not lead to statistically significant variations in microbial communities. The consistent presence of these genera across all samples suggests that they are stable components of the grapevine endophytic microbiota, remaining relatively unaffected by external environmental factors.
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Claims 7
Claim · model-written summary
Culture-based methods identified 148 grapevine endophytes.
- Taxon
- Vitis vinifera
Vitis viniferat0 - Outcome
- endophytese0
- Value
- 148e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through culture-based methods, 148 endophytes were identified.”
t0“Vitis vinifera”
Claim · model-written summary
Aureobasidium pullulans, Alternaria alternata and Cladosporium allicinum were dominant cultured fungi.
- Taxon
- Vitis viniferaVitis viniferat0
- Interactor
- Aureobasidium pullulans
Aureobasidium pullulanse0symbiont - Second interactor
- Alternaria alternata
.jpg?width=60)
Alternaria alternatae0symbiont - Outcome
- dominant fungal speciese0
- Qualifier
- Cladosporium allicinume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The dominant fungal species included Aureobasidium pullulans, Alternaria alternata, and Cladosporium allicinum”
t0“Vitis vinifera”
Claim · model-written summary
Ralstonia pikettii, Nocardia niigatensis and Sphingomonas echinoides were predominant cultured bacteria.
- Taxon
- Vitis viniferaVitis viniferat0
- Interactor
- Ralstonia pikettiie0symbiont
- Second interactor
- Nocardia niigatensisNocardia niigatensise0symbiont
- Outcome
- predominant bacterial speciese0
- Qualifier
- Sphingomonas echinoidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“predominant bacterial species were Ralstonia pikettii, Nocardia niigatensis, and Sphingomonas echinoides.”
t0“Vitis vinifera”
Claim · model-written summary
Halomonas sp., Sphingomonas sp. and Massilia sp. were main bacteria identified by culture-independent methods.
- Taxon
- Vitis viniferaVitis viniferat0
- Second interactor
- Sphingomonas sp.
Sphingomonase0symbiont - Outcome
- main bacterial species identifiede0
- Qualifier
- Massilia sp.e0
.jpg?width=60){kind=small}
HalomonasOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The main bacterial species identified included Halomonas sp., Sphingomonas sp. and Massilia sp.”
t0“Vitis vinifera”
Claim · model-written summary
Cladosporium sp., Malassezia sp. and Mucor sp. dominated the culture-independent fungal population.
- Taxon
- Vitis viniferaVitis viniferat0
- Interactor
- Cladosporium sp.
Cladosporiume0symbiont - Second interactor
- Malassezia sp.
Malasseziae0symbiont - Outcome
- fungal populatione0
- Qualifier
- Mucor sp.e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the fungal population was dominated by Cladosporium sp., Malassezia sp. and Mucor sp.”
t0“Vitis vinifera”
Claim · model-written summary
Vineyard management practices did not cause statistically significant variation in microbial communities.
- Taxon
- Vitis viniferaVitis viniferat0
- Treatment
- vineyard management practicese0
- Outcome
- microbial communitiese0
- Direction
- did not lead to statistically significant variationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vineyard management practices did not lead to statistically significant variations in microbial communities.”
t0“Vitis vinifera”
Claim · model-written summary
The same genera consistently occurred across all grapevine samples.
- Outcome
- consistent presence of these genera across all samplese0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The consistent presence of these genera across all samples suggests that they are stable components of the grapevine endophytic microbiota”