Abstract
Codonopsis Radix, a medicinal and dietary herb in traditional Chinese medicine, largely owes its pharmacological efficacy to both intrinsic phytochemistry and symbiotic interactions with plant-associated microbes. Here, we deciphered the geo-environmental regulation of Codonopsis Radix's endophytic microbiota across four major production regions using 16S rRNA/ITS sequencing and bioactive compound profiling. Results demonstrated that the planting environment significantly shaped the endophytic community of Codonopsis Radix, where Bifidobacteriaceae and Muribaculaceae exhibited the strongest correlations with its bioactive components. Monolobus and Bradyrhizobium not only exhibit distinct associations with Lobetyolin and Atractylenolide III respectively, but also demonstrate significant correlations with the key biosynthetic pathways of these compounds. Leveraging machine learning, we developed the first microbiota-driven quality assessment model, achieving 100.0% and 85.7% prediction accuracies for Lobetyolin and Atractylenolide III respectively, using Random Forest algorithms. This dual-metric framework-integrating microbial signatures with chemical profiles-establishes a novel paradigm for Codonopsis Radix quality control, bridging ecological insights with precision agriculture. Our findings illuminate the microbiota's role as a biosynthetic orchestrator in geoherbalism, offering actionable strategies for sustainable cultivation and standardized production of Codonopsis Radix.
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Claims 9
Claim · model-written summary
Planting environment significantly shaped the Codonopsis endophytic community.
Verbatim
e0“planting environment significantly shaped the endophytic community of Codonopsis Radix”
Claim · model-written summary
Bifidobacteriaceae had one of the strongest correlations with Codonopsis bioactive components.
- Taxon
- Codonopsis Radixe1
- Interactor
- BifidobacteriaceaeBifidobacteriaceaee0symbiont
- Outcome
- bioactive componentse0
- Qualifier
- strongest correlationse0
Not reportedDirection
Verbatim
e0“Bifidobacteriaceae and Muribaculaceae exhibited the strongest correlations with its bioactive components”
e1“endophytic community of Codonopsis Radix”
Claim · model-written summary
Muribaculaceae had one of the strongest correlations with Codonopsis bioactive components.
- Taxon
- Codonopsis Radixe1
- Interactor
- MuribaculaceaeMuribaculaceaee0symbiont
- Outcome
- bioactive componentse0
- Qualifier
- strongest correlationse0
Not reportedDirection
Verbatim
e0“Bifidobacteriaceae and Muribaculaceae exhibited the strongest correlations with its bioactive components”
e1“endophytic community of Codonopsis Radix”
Claim · model-written summary
Monolobus was associated with Lobetyolin.
Not reportedDirection
Verbatim
e0“Monolobus and Bradyrhizobium not only exhibit distinct associations with Lobetyolin and Atractylenolide III respectively”
e1“endophytic community of Codonopsis Radix”
Claim · model-written summary
Bradyrhizobium was associated with Atractylenolide III.
- Taxon
- Codonopsis Radixe1
- Interactor
- Bradyrhizobium
Bradyrhizobiume0symbiont - Outcome
- Atractylenolide IIIe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Monolobus and Bradyrhizobium not only exhibit distinct associations with Lobetyolin and Atractylenolide III respectively”
e1“endophytic community of Codonopsis Radix”
Claim · model-written summary
Monolobus correlated significantly with a key biosynthetic pathway.
Not reportedDirection
Verbatim
e0“Monolobus and Bradyrhizobium not only exhibit distinct associations with Lobetyolin and Atractylenolide III respectively, but also demonstrate significant correlations with the key biosynthetic pathways of these compounds.”
e1“endophytic community of Codonopsis Radix”
Claim · model-written summary
Bradyrhizobium correlated significantly with a key biosynthetic pathway.
- Taxon
- Codonopsis Radixe1
- Interactor
- BradyrhizobiumBradyrhizobiume0symbiont
- Outcome
- key biosynthetic pathways of these compoundse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Monolobus and Bradyrhizobium not only exhibit distinct associations with Lobetyolin and Atractylenolide III respectively, but also demonstrate significant correlations with the key biosynthetic pathways of these compounds.”
e1“endophytic community of Codonopsis Radix”
Claim · model-written summary
Random Forest predicted Lobetyolin quality at 100.0% accuracy.
Verbatim
e0“endophytic community of Codonopsis Radix”
e1“achieving 100.0% and 85.7% prediction accuracies for Lobetyolin and Atractylenolide III respectively, using Random Forest algorithms”
Claim · model-written summary
Random Forest predicted Atractylenolide III quality at 85.7% accuracy.
Verbatim
e0“endophytic community of Codonopsis Radix”
e1“achieving 100.0% and 85.7% prediction accuracies for Lobetyolin and Atractylenolide III respectively, using Random Forest algorithms”