Abstract
Background Idesia polycarpa Maxim. is a high-quality, high-yield, edible oil tree species native to eastern Asia, where it plays important roles in ensuring national food and oil security, promoting ecological development, and facilitating rural revitalization. However, the commercial development of I . polycarpa has been hampered by the fact that it is primarily propagated by seeds, the required dormancy of which leads to low natural germination rates. Tissue culture technology offers the advantages of rapid propagation, high multiplication rates, and independence from seasonal factors, enabling the rapid production of large quantities of high-quality seedlings. The aim of this study was to establish an efficient aseptic germination system for I. polycarpa seeds. Methods This study utilized I . polycarpa seeds as the experimental material to investigate the effects of different disinfection times, basic medium variations, activated carbon (AC) concentrations, and the types and concentrations of plant growth regulators (PGRs) on aseptic germination. Subsequently, sterile seedlings were used as explants to screen for the effects of sucrose concentration and the types and concentrations of PGRs on rooting. The study also investigated how different substrate ratios and container types influenced the post-transplant survival rate of tissue-cultured I . polycarpa seedlings. Results The results showed that the optimal time was 10 min for I . polycarpa seed disinfection with 0.1% HgCl 2 . The most suitable medium for I . polycarpa seed germination was 1/2 MS medium supplemented with GA 3 (1.0 mg·L −1 ) and AC (1.0 g·L −1 ), achieving a germination rate of 96.0%. A sucrose concentration of 10.0 g·L −1 was most beneficial for rooting. When using a single plant growth regulator, indole-3-butyric acid (IBA) had the most significant effect on I . polycarpa root induction. The optimal medium for root development was Murashige and Skoog (MS) medium supplemented with IBA (0.3 mg·L −1 ) and α-naphthyl acetic acid (NAA) (0.5 mg·L −1 ), resulting in a 100% rooting rate and an average of 22.17 roots. These roots had an average length of 3.4 cm and were abundant and vigorous. Tissue-cultured seedlings were transplanted into transparent plastic cups containing a mixed substrate of organic nutrient soil (BALTIC PEAT), perlite, and vermiculite in a ratio of 2:1:1 (V/V/V). They grew vigorously, with a survival rate as high as 96.67%. The findings of this study can provide technical support for the factory breeding of I . polycarpa seedlings.
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Claims 8
Claim · model-written summary
Disinfecting Idesia polycarpa seeds for 10 min with 0.1% HgCl2 was the optimal time.
- Taxon
- Idesia polycarpa
Idesia polycarpat0 - Treatment
- 0.1% HgCl 2e1
- Outcome
- optimal timee1
- Value
- 10 mine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e1“the optimal time was 10 min for I . polycarpa seed disinfection with 0.1% HgCl 2”
Claim · model-written summary
1/2 MS medium with GA3 and activated carbon gave a seed germination rate of 96.0%.
- Taxon
- Idesia polycarpaIdesia polycarpat0
- Treatment
- 1/2 MS medium supplemented with GA 3 (1.0 mg·L −1 ) and AC (1.0 g·L −1 )e2
- Outcome
- germination ratee2
- Value
- 96.0%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e2“The most suitable medium for I . polycarpa seed germination was 1/2 MS medium supplemented with GA 3 (1.0 mg·L −1 ) and AC (1.0 g·L −1 ), achieving a germination rate of 96.0%”
Claim · model-written summary
A sucrose concentration of 10.0 g/L was most beneficial for rooting of Idesia polycarpa.
- Taxon
- Idesia polycarpaIdesia polycarpat0
- Treatment
- sucrose concentration of 10.0 g·L −1e3
- Outcome
- rootinge3
- Direction
- most beneficiale3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e3“A sucrose concentration of 10.0 g·L −1 was most beneficial for rooting”
Claim · model-written summary
Indole-3-butyric acid had the most significant effect on root induction of Idesia polycarpa among single growth regulators.
- Taxon
- Idesia polycarpaIdesia polycarpat0
- Treatment
- indole-3-butyric acid (IBA)e4
- Outcome
- root inductione4
- Direction
- most significant effecte4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e4“indole-3-butyric acid (IBA) had the most significant effect on I . polycarpa root induction”
Claim · model-written summary
MS medium with IBA and NAA gave a 100% rooting rate of Idesia polycarpa.
- Taxon
- Idesia polycarpaIdesia polycarpat0
- Treatment
- IBA (0.3 mg·L −1 ) and α-naphthyl acetic acid (NAA) (0.5 mg·L −1 )e5
- Outcome
- rooting ratee5
- Value
- 100%e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e5“The optimal medium for root development was Murashige and Skoog (MS) medium supplemented with IBA (0.3 mg·L −1 ) and α-naphthyl acetic acid (NAA) (0.5 mg·L −1 ), resulting in a 100% rooting rate and an average of 22.17 roots”
Claim · model-written summary
The same MS medium gave an average of 22.17 roots per Idesia polycarpa explant.
- Taxon
- Idesia polycarpaIdesia polycarpat0
- Treatment
- IBA (0.3 mg·L −1 ) and α-naphthyl acetic acid (NAA) (0.5 mg·L −1 )e5
- Outcome
- rootse5
- Value
- 22.17 rootse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e5“The optimal medium for root development was Murashige and Skoog (MS) medium supplemented with IBA (0.3 mg·L −1 ) and α-naphthyl acetic acid (NAA) (0.5 mg·L −1 ), resulting in a 100% rooting rate and an average of 22.17 roots”
Claim · model-written summary
Roots of Idesia polycarpa had an average length of 3.4 cm.
- Taxon
- Idesia polycarpaIdesia polycarpat0
- Outcome
- average lengthe6
- Value
- 3.4 cme6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e6“These roots had an average length of 3.4 cm”
Claim · model-written summary
Tissue-cultured Idesia polycarpa seedlings transplanted into the 2:1:1 peat, perlite and vermiculite substrate had a survival rate of 96.67%.
- Taxon
- Idesia polycarpaIdesia polycarpat0
- Treatment
- mixed substrate of organic nutrient soil (BALTIC PEAT), perlite, and vermiculite in a ratio of 2:1:1 (V/V/V)e2
- Outcome
- survival ratee1
- Value
- 96.67%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Idesia polycarpa”
e1“with a survival rate as high as 96.67%”
e2“transplanted into transparent plastic cups containing a mixed substrate of organic nutrient soil (BALTIC PEAT), perlite, and vermiculite in a ratio of 2:1:1 (V/V/V)”