Abstract
Abstract Fusarium dry rot is a significant fungal disease impacting postharvest potato tubers worldwide, leading to substantial losses in tuber quality, nutritional value, and seed viability. In Kyrgyzstan, where potato is a staple crop, the disease poses a critical threat to food security. This study aimed to identify and characterize Fusarium species associated with dry rot in stored potatoes from the Osh region, a major potato production area facing severe postharvest challenges. Surveys were conducted in 34 potato storage facilities across four districts, and symptomatic tubers were collected. Fifty-four Fusarium isolates were obtained and subjected to a comprehensive characterization approach, including morphological assessments, molecular analyses using iPBS markers based on retrotransposons, DNA sequencing of the translation elongation factor-1 alpha ( TEF1 ) locus, and pathogenicity tests on the ‘Soraya’ potato cultivar. Fusarium sambucinum was the most prevalent species (50%), followed by F. oxysporum (~ 17%), F. flocciferum (~ 11%), F. equiseti (~ 9%), F. proliferatum (~ 5%), F. acuminatum (~ 4%), F. solani (~ 2%), and F. venenatum (~ 2%). Morphological identifications were supported by iPBS2395 primer analysis, which revealed distinct DNA fingerprinting patterns among the isolates. Phylogenetic analysis of TEF1 gene sequences further confirmed species identifications, demonstrating strong congruence between morphological, iPBS, and TEF1 data. Pathogenicity tests revealed varying levels of virulence across species, with F. sambucinum exhibiting the highest overall virulence and mean lesion sizes comparable to those of F. flocciferum , while F. equiseti and F. solani showed significantly lower virulence. Except for F. sambucinum , all other Fusarium species represent first reports in Kyrgyzstan, with F. flocciferum being reported globally for the first time as a causal agent of potato dry rot. This study provides a comprehensive assessment of the Fusarium species involved in dry rot in the Osh region, highlighting the dominance of F. sambucinum and the need for enhanced management strategies to mitigate postharvest losses and strengthen food security in Kyrgyzstan.
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Claims 16
Claim · model-written summary
Fusarium sambucinum was the most prevalent isolate at 50%.
- Taxon
- Fusarium sambucinumFusarium sambucinume0
- Outcome
- most prevalent speciese0
- Value
- 50%e0
- Setting
- Kyrgyzstane1
Verbatim
e0“Fusarium sambucinum was the most prevalent species (50%),”
e1“In Kyrgyzstan, where potato is a staple crop”
Claim · model-written summary
F. oxysporum accounted for about 17% of isolates.
- Taxon
- F. oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0 - Outcome
- F. oxysporume0
- Value
- ~ 17%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“followed by F. oxysporum (~ 17%)”
Claim · model-written summary
F. flocciferum accounted for about 11% of isolates.
- Taxon
- F. flocciferumFusarium flocciferume0
- Outcome
- F. flocciferume0
- Value
- ~ 11%e0
Verbatim
e0“F. flocciferum (~ 11%)”
Claim · model-written summary
F. equiseti accounted for about 9% of isolates.
- Taxon
- F. equisetiFusarium equisetie0
- Outcome
- F. equisetie0
- Value
- ~ 9%e0
Verbatim
e0“F. equiseti (~ 9%)”
Claim · model-written summary
F. proliferatum accounted for about 5% of isolates.
- Taxon
- F. proliferatumFusarium proliferatume0
- Outcome
- F. proliferatume0
- Value
- ~ 5%e0
Verbatim
e0“F. proliferatum (~ 5%)”
Claim · model-written summary
F. acuminatum accounted for about 4% of isolates.
- Taxon
- F. acuminatum
Fusarium acuminatume0 - Outcome
- F. acuminatume0
- Value
- ~ 4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F. acuminatum (~ 4%)”
Claim · model-written summary
F. solani accounted for about 2% of isolates.
- Taxon
- F. solani
_Sacc._5393379.jpg?width=60)
Fusarium solanie0 - Outcome
- F. solanie0
- Value
- ~ 2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Sacc._5393379.jpg?width=500)
Verbatim
e0“F. solani (~ 2%)”
Claim · model-written summary
F. venenatum accounted for about 2% of isolates.
- Taxon
- F. venenatumFusarium venenatume0
- Outcome
- F. venenatume0
- Value
- ~ 2%e0
Verbatim
e0“F. venenatum (~ 2%)”
Claim · model-written summary
Morphological identifications were supported by distinct iPBS2395 DNA fingerprints.
FusariumNot reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Morphological identifications were supported by iPBS2395 primer analysis, which revealed distinct DNA fingerprinting patterns among the isolates.”
e1“Fifty-four Fusarium isolates were obtained”
Claim · model-written summary
TEF1 phylogeny confirmed the species identifications and agreed with morphology and iPBS.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis of TEF1 gene sequences further confirmed species identifications, demonstrating strong congruence between morphological, iPBS, and TEF1 data.”
e1“Fifty-four Fusarium isolates were obtained”
Claim · model-written summary
F. sambucinum had the highest overall virulence on potato.
- Taxon
- F. sambucinumFusarium sambucinume0
- Interactor
- potato
Solanum tuberosume1host - Outcome
- virulencee0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F. sambucinum exhibiting the highest overall virulence”
e1“pathogenicity tests on the ‘Soraya’ potato cultivar”
Claim · model-written summary
F. sambucinum lesion sizes were comparable to F. flocciferum.
- Taxon
- F. sambucinumFusarium sambucinume0
- Outcome
- mean lesion sizese0
- Direction
- comparablee0
- Comparator
- F. flocciferume0
Verbatim
e0“F. sambucinum exhibiting the highest overall virulence and mean lesion sizes comparable to those of F. flocciferum”
Claim · model-written summary
F. equiseti showed significantly lower virulence.
- Taxon
- F. equisetiFusarium equisetie0
- Outcome
- virulencee0
- Direction
- lowere0
Verbatim
e0“F. equiseti and F. solani showed significantly lower virulence”
Claim · model-written summary
F. solani showed significantly lower virulence.
- Taxon
- F. solaniFusarium solanie0
- Outcome
- virulencee0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F. equiseti and F. solani showed significantly lower virulence”
Claim · model-written summary
All Fusarium species except F. sambucinum were first reported in Kyrgyzstan.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Except for F. sambucinum , all other Fusarium species represent first reports in Kyrgyzstan”
Claim · model-written summary
F. flocciferum was first reported globally as a causal agent of potato dry rot.
- Taxon
- F. flocciferumFusarium flocciferume0
- Interactor
- potatoSolanum tuberosume0host
- Outcome
- causal agent of potato dry rote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F. flocciferum being reported globally for the first time as a causal agent of potato dry rot”