Abstract
To understand why close wild plant relatives of crops, such as Solanum dulcamara, are resistant to Ralstonia solanacearum we need genome resources to perform comparative studies and identify key genes and pathways. We de-novo assembled and annotated the genome of a resistant/tolerant S. dulcamara and susceptible Solanum nigrum plant using a hybrid approach including Oxford Nanopore Technologies and Illumina sequencing. Comparative genomic analysis was then performed to find differences between the genome of S. dulcamara and other susceptible Solanaceous species including potato, tomato, aubergine, and S. nigrum and one susceptible and one resistant S. americanum accession. We identified genes associated with auxin-transport only in S. dulcamara and a collection of pattern recognition receptors (PRRs) in orthogroups only found in resistant/tolerant plant species, which we hypothesize may improve recognition of pathogen-associated molecular patterns associated with R. solanacearum. We also identified an arsenal of nucleotide-binding leucine-rich repeat receptors (NLRs) in the S. nigrum genome that are shared with the other susceptible species and could be acting as susceptibility factors. Finally, we identified differences in methylation frequency across the gene bodies in both species, which may be associated with epigenetic regulation of resistance. Future work should assess the functional role of these PRRs and NLRs during bacterial wilt development to determine if they could offer potential novel targets for breeding to improve bacterial wilt resistance.
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Claims 6
Claim · model-written summary
A resistant or tolerant S. dulcamara genome was assembled and annotated.
- Taxon
- S. dulcamara
Solanum dulcamarae0 - Outcome
- assembled and annotated the genomee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“assembled and annotated the genome of a resistant/tolerant S. dulcamara”
Claim · model-written summary
A susceptible Solanum nigrum genome was assembled and annotated.
- Taxon
- Solanum nigrum
_Solanum_nigrum_3.jpg?width=60)
Solanum nigrume0 - Outcome
- hybrid approach including Oxford Nanopore Technologies and Illumina sequencinge0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Solanum_nigrum_3.jpg?width=500)
Verbatim
e0“susceptible Solanum nigrum plant using a hybrid approach including Oxford Nanopore Technologies and Illumina sequencing”
Claim · model-written summary
Genes associated with auxin transport were identified only in S. dulcamara.
- Taxon
- S. dulcamaraSolanum dulcamarae0
- Gene
- genes associated with auxin-transporte0
- Outcome
- only in S. dulcamarae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes associated with auxin-transport only in S. dulcamara”
Claim · model-written summary
PRR orthogroups were found only in resistant or tolerant plant species.
Verbatim
e0“pattern recognition receptors (PRRs) in orthogroups only found in resistant/tolerant plant species”
Claim · model-written summary
NLRs in S. nigrum were shared with other susceptible species.
- Taxon
- S. nigrumSolanum nigrume0
- Gene
- nucleotide-binding leucine-rich repeat receptors (NLRs)e0
- Outcome
- shared with the other susceptible speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nucleotide-binding leucine-rich repeat receptors (NLRs) in the S. nigrum genome that are shared with the other susceptible species”
Claim · model-written summary
The two nightshades differed in gene-body methylation frequency.
- Taxon
- Solanum dulcamara and Solanum nigrumSolanum nigrumSolanum dulcamarat0
- Outcome
- methylation frequency across the gene bodiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differences in methylation frequency across the gene bodies in both species”
t0“Solanum dulcamara and Solanum nigrum”