Abstract
Non-ribosomal peptide (NRP) producing bacteria confer promising alternatives for control of plant pathogens. In the current study Serratia surfactantfaciens (S31) and Alcaligenes pakistanensis (S33) were isolated from the rhizosphere of Solanum lycopersicum. The main aim of the study was to explore the antifungal potential of these novel strains, to characterize the synthesized NRPs and determine their antibiosis mechanisms and influence on plant growth. To screen the strains for their antifungal potential, dual assays were performed against the plant pathogens Aspergillus niger , A . flavus , A . fumigatus and Fusarium oxysporum. When screened for quantitative results regarding enzyme production, A . pakistanensis exhibited a zone of 38.50±4.95 mm for pectinase whereas S . surfactantfaciens showed a zone of 23.0±2.82 mm for cellulase. Antifungal assays showed prominent zones of growth inhibition of the phytopathogens using both bacterial cultures and cell-free supernatants. Genetic analysis revealed the presence of various NRP genes in the bacterial genomes, which were further confirmed by structural analysis. In vivo experimentation showed higher seedling vigour and plant growth parameters when grown in autoclaved as compared to non-autoclaved soil for the applied treatments.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Alcaligenes pakistanensis showed a pectinase zone of 38.50±4.95 mm in the quantitative enzyme screening.
- Taxon
- A . pakistanensisAlcaligenes pakistanensise0
- Outcome
- zonee0
- Value
- 38.50±4.95 mme0
- Qualifier
- pectinasee0
Verbatim
e0“A . pakistanensis exhibited a zone of 38.50±4.95 mm for pectinase”
Claim · model-written summary
Serratia surfactantfaciens showed a cellulase zone of 23.0±2.82 mm in the quantitative enzyme screening.
- Taxon
- S . surfactantfaciensSerratia surfactantfaciense0
- Outcome
- zonee0
- Value
- 23.0±2.82 mme0
- Qualifier
- cellulasee0
Verbatim
e0“S . surfactantfaciens showed a zone of 23.0±2.82 mm for cellulase”
Claim · model-written summary
Both bacterial cultures and cell-free supernatants produced prominent zones of growth inhibition of the plant pathogenic fungi in antifungal assays.
Verbatim
e0“Antifungal assays showed prominent zones of growth inhibition of the phytopathogens using both bacterial cultures and cell-free supernatants”
Claim · model-written summary
Various NRP genes were present in the genomes of the two bacterial strains, as revealed by genetic analysis.
Verbatim
e0“Genetic analysis revealed the presence of various NRP genes in the bacterial genomes”
Claim · model-written summary
In vivo, seedling vigour and plant growth parameters were higher in autoclaved than in non-autoclaved soil for the applied treatments.
- Treatment
- autoclavede0
- Outcome
- seedling vigour and plant growth parameterse0
- Direction
- highere0
- Comparator
- non-autoclaved soile0
Not reportedTaxon
Verbatim
e0“In vivo experimentation showed higher seedling vigour and plant growth parameters when grown in autoclaved as compared to non-autoclaved soil for the applied treatments”