Abstract
Abstract Sweet potato ( Ipomoea batatas ) is a key staple crop in Costa Rica, but its vegetative propagation renders it highly vulnerable to viral infections. Sweet potato virus disease (SPVD), one of the most damaging viral syndromes, arises from co-infection by sweet potato feathery mottle virus (SPFMV; genus Potyvirus ) and sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus ). This preliminary study investigated SPFMV accumulation in three sweet potato genotypes—Criollo (CRL), Guapileño (GPN) and Beauregard (BRD)—naturally co-infected with SPCSV and examined associated cytopathological alterations. Field samples exhibiting SPVD-like symptoms were confirmed to be co-infected with SPFMV (lineage B) and SPCSV (WA strain) through multiplex reverse transcription PCR and Sanger sequencing. A two-step quantitative reverse transcription PCR (RT-qPCR) targeting the SPFMV coat protein gene was used to quantify viral load. CRL exhibited significantly higher SPFMV titers (5.06 × 10⁶ copies/mg tissue) compared to GPN and BRD. Electron microscopy of CRL leaf tissue revealed typical pinwheel inclusion bodies and chloroplast deformation, consistent with potyvirus-induced cytopathology. These findings reveal differences in SPFMV accumulation among genotypes under field co-infection conditions, which may be influenced by multiple factors including host background and environment.
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Claims 4
Claim · model-written summary
Symptomatic field sweet potato samples were confirmed co-infected with SPFMV lineage B and SPCSV WA strain.
- Taxon
- sweet potato
_Flower.jpg?width=60)
Ipomoea batatast0 - Interactor
- SPFMVSweet potato feathery mottle viruse0pathogen
- Second interactor
- SPCSVe0pathogen
- Outcome
- co-infected with SPFMV (lineage B) and SPCSV (WA strain)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
e0“Field samples exhibiting SPVD-like symptoms were confirmed to be co-infected with SPFMV (lineage B) and SPCSV (WA strain) through multiplex reverse transcription PCR and Sanger sequencing”
t0“field-collected sweet potato genotypes”
Claim · model-written summary
Criollo had significantly higher SPFMV titers than Guapileño and Beauregard, at 5.06 × 10⁶ copies/mg tissue.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- CRLe0
- Interactor
- SPFMVSweet potato feathery mottle viruse0pathogen
- Outcome
- SPFMV titerse0
- Direction
- highere0
- Value
- 5.06 × 10⁶ copies/mg tissuee0
- Comparator
- GPN and BRDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRL exhibited significantly higher SPFMV titers (5.06 × 10⁶ copies/mg tissue) compared to GPN and BRD”
t0“field-collected sweet potato genotypes”
Claim · model-written summary
Criollo leaf tissue displayed pinwheel inclusion bodies.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- CRLe0
- Organ / stage
- leaf tissuee0
- Outcome
- pinwheel inclusion bodiese0
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Electron microscopy of CRL leaf tissue revealed typical pinwheel inclusion bodies and chloroplast deformation”
t0“field-collected sweet potato genotypes”
Claim · model-written summary
Criollo leaf tissue displayed chloroplast deformation.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- CRLe0
- Organ / stage
- leaf tissuee0
- Outcome
- chloroplast deformatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Electron microscopy of CRL leaf tissue revealed typical pinwheel inclusion bodies and chloroplast deformation”
t0“field-collected sweet potato genotypes”