Abstract
Sweet pea (Lathyrus odoratus), an important ornamental plant, has been hindered in functional genomics and genetic breeding due to the lack of an efficient transformation and genome editing system. In this study, we developed a simple and efficient CRISPR/Cas9 gene-editing system in sweet pea using an Agrobacterium rhizogenes-mediated (ARM) transformation approach. We constructed a sweet pea genome editing vector, LoGE1, which incorporates a CRISPR/Cas9 system with AtU6-driven sgRNA and CaMV35S-driven Cas9 expression, along with a green fluorescent protein (GFP) reporter. The constructs LoGE1-PDS and LoGE1-SD1 were designed to target LoPDS and LoSD1, respectively, and were introduced into sweet pea using Agrobacterium rhizogenes K599-mediated transformation. Transformation efficiency was evaluated through GFP fluorescence, achieving 54% for LoGE1-PDS and 71% for LoGE1-SD1. Genome editing efficiency at the target sites was 71% for LoPDS and 60% for LoSD1, with various mutation patterns observed. This study provides a foundation for gene editing in sweet pea and offers a reference for developing similar systems in other ornamental plants. The first genome editing system for sweet pea was established using a simple and efficient Agrobacterium rhizogenes-mediated method, achieving high editing efficiency with various mutation patterns.
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Claims 5
Claim · model-written summary
LoGE1-PDS transformation efficiency was 54%.
- Taxon
- sweet pea
Lathyrus odoratust0 - Material
- LoGE1-PDSe0
- Outcome
- Transformation efficiencye0
- Value
- 54%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation efficiency was evaluated through GFP fluorescence, achieving 54% for LoGE1-PDS and 71% for LoGE1-SD1”
t0“sweet pea (Lathyrus odoratus)”
Claim · model-written summary
LoGE1-SD1 transformation efficiency was 71%.
- Taxon
- sweet peaLathyrus odoratust0
- Material
- LoGE1-SD1e0
- Outcome
- Transformation efficiencye0
- Value
- 71%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation efficiency was evaluated through GFP fluorescence, achieving 54% for LoGE1-PDS and 71% for LoGE1-SD1”
t0“sweet pea (Lathyrus odoratus)”
Claim · model-written summary
Editing efficiency at LoPDS was 71%.
- Taxon
- sweet peaLathyrus odoratust0
- Gene
- LoPDSe0
- Outcome
- Genome editing efficiencye0
- Value
- 71%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genome editing efficiency at the target sites was 71% for LoPDS and 60% for LoSD1, with various mutation patterns observed”
t0“sweet pea (Lathyrus odoratus)”
Claim · model-written summary
Editing efficiency at LoSD1 was 60%.
- Taxon
- sweet peaLathyrus odoratust0
- Gene
- LoSD1e0
- Outcome
- Genome editing efficiencye0
- Value
- 60%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genome editing efficiency at the target sites was 71% for LoPDS and 60% for LoSD1, with various mutation patterns observed”
t0“sweet pea (Lathyrus odoratus)”
Claim · model-written summary
Genome editing produced varied mutation patterns.
- Taxon
- sweet peaLathyrus odoratust0
- Outcome
- various mutation patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with various mutation patterns observed”
e1“Genome editing efficiency at the target sites was 71% for LoPDS and 60% for LoSD1”
t0“sweet pea (Lathyrus odoratus)”