Abstract
Abstract This study investigates the prevalence and genetic characterization of Grapevine Fleck Virus (GFkV) in major Algerian vineyards. A total of 430 grapevine samples from 12 regions were analyzed using serological (DAS-ELISA), molecular (RT-PCR), and high-throughput sequencing (Illumina-NGS) methods. The results revealed a high overall infection rate of 65.8%, within the central and western regions showing the highest prevalence, while no infections were detected in the southern regions. Significant differences in infection rates were observed among grapevine cultivars. Genetic analysis of Algerian GFkV isolates confirmed the presence of conserved genomic regions and identified new sequence data submitted to GenBank. Phylogenetic analysis revealed that the Italian and Algerian isolates are related. These findings underscore the importance of integrating virus monitoring into national certification and plant health programs to safeguard the sustainability and productivity of Algeria's viticulture. Continued research and collaboration will be essential to mitigate the impact of viral diseases and ensure the long-term resilience of the country's grapevine industry.
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Claims 7
Claim · model-written summary
Overall GFkV infection in grapevine samples was 65.8%.
- Interactor
- Grapevine Fleck Virus (GFkV)Grapevine fleck virust0pathogen
- Outcome
- infection ratee0
- Value
- 65.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results revealed a high overall infection rate of 65.8%”
t0“Grapevine Fleck Virus (GFkV) infection”
t1“grapevine samples from 12 regions”
Claim · model-written summary
Central and western Algerian regions had the highest GFkV prevalence.
- Interactor
- Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
- Outcome
- prevalencee0
- Direction
- higheste0
- Setting
- central and western regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the central and western regions showing the highest prevalence”
t0“grapevine samples from 12 regions”
t1“Grapevine Fleck Virus (GFkV)”
Claim · model-written summary
No GFkV infections were detected in southern regions.
- Interactor
- Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
- Outcome
- infectionse0
- Setting
- southern regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no infections were detected in the southern regions”
t0“grapevine samples from 12 regions”
t1“Grapevine Fleck Virus (GFkV)”
Claim · model-written summary
Algerian GFkV isolates had conserved genomic regions.
- Interactor
- GFkVGrapevine fleck viruse0pathogen
- Outcome
- conserved genomic regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genetic analysis of Algerian GFkV isolates confirmed the presence of conserved genomic regions”
t0“grapevine samples from 12 regions”
Claim · model-written summary
New Algerian GFkV sequence data were submitted to GenBank.
- Interactor
- Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
- Outcome
- new sequence data submitted to GenBanke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified new sequence data submitted to GenBank”
t0“grapevine samples from 12 regions”
t1“Grapevine Fleck Virus (GFkV)”
Claim · model-written summary
Italian and Algerian GFkV isolates were phylogenetically related.
- Interactor
- Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
- Outcome
- Italian and Algerian isolates are relatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis revealed that the Italian and Algerian isolates are related”
t0“grapevine samples from 12 regions”
t1“Grapevine Fleck Virus (GFkV)”
Claim · model-written summary
The authors urged virus monitoring in national certification and plant health programs.
- Interactor
- Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
- Outcome
- integrating virus monitoring into national certification and plant health programse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“integrating virus monitoring into national certification and plant health programs”
t0“grapevine samples from 12 regions”
t1“Grapevine Fleck Virus (GFkV)”