Paper · 2025 · Acta Phytopathologica et Entomologica Hungarica

Assessment of Grapevine Fleck Virus (GFkV) infection in Algerian vineyards

Imene Mahdid; Osamah Alisawi; Anfel Djenaoui; et al.

7
claims
20
verbatim quotes
1
citations
4
research areas
—
Not open access

Abstract

Abstract This study investigates the prevalence and genetic characterization of Grapevine Fleck Virus (GFkV) in major Algerian vineyards. A total of 430 grapevine samples from 12 regions were analyzed using serological (DAS-ELISA), molecular (RT-PCR), and high-throughput sequencing (Illumina-NGS) methods. The results revealed a high overall infection rate of 65.8%, within the central and western regions showing the highest prevalence, while no infections were detected in the southern regions. Significant differences in infection rates were observed among grapevine cultivars. Genetic analysis of Algerian GFkV isolates confirmed the presence of conserved genomic regions and identified new sequence data submitted to GenBank. Phylogenetic analysis revealed that the Italian and Algerian isolates are related. These findings underscore the importance of integrating virus monitoring into national certification and plant health programs to safeguard the sustainability and productivity of Algeria's viticulture. Continued research and collaboration will be essential to mitigate the impact of viral diseases and ensure the long-term resilience of the country's grapevine industry.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Overall GFkV infection in grapevine samples was 65.8%.

Taxon
grapevineVitist1
Interactor
Grapevine Fleck Virus (GFkV)Grapevine fleck virust0pathogen
Outcome
infection ratee0
Value
65.8%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The results revealed a high overall infection rate of 65.8%”

✓ located verbatim in the source · abstract, characters 307–366 · show in text ↑
t0

“Grapevine Fleck Virus (GFkV) infection”

✓ located verbatim in the source · title, characters 14–52 · show in text ↑
t1

“grapevine samples from 12 regions”

✓ located verbatim in the source · abstract, characters 153–186 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Central and western Algerian regions had the highest GFkV prevalence.

Taxon
grapevineVitist0
Interactor
Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
Outcome
prevalencee0
Direction
higheste0
Setting
central and western regionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the central and western regions showing the highest prevalence”

✓ located verbatim in the source · abstract, characters 375–437 · show in text ↑
t0

“grapevine samples from 12 regions”

✓ located verbatim in the source · abstract, characters 153–186 · show in text ↑
t1

“Grapevine Fleck Virus (GFkV)”

✓ located verbatim in the source · title, characters 14–42 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No GFkV infections were detected in southern regions.

Taxon
grapevineVitist0
Interactor
Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
Outcome
infectionse0
Setting
southern regionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e0

“no infections were detected in the southern regions”

✓ located verbatim in the source · abstract, characters 445–496 · show in text ↑
t0

“grapevine samples from 12 regions”

✓ located verbatim in the source · abstract, characters 153–186 · show in text ↑
t1

“Grapevine Fleck Virus (GFkV)”

✓ located verbatim in the source · title, characters 14–42 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Algerian GFkV isolates had conserved genomic regions.

Taxon
grapevineVitist0
Interactor
GFkVGrapevine fleck viruse0pathogen
Outcome
conserved genomic regionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Genetic analysis of Algerian GFkV isolates confirmed the presence of conserved genomic regions”

✓ located verbatim in the source · abstract, characters 582–676 · show in text ↑
t0

“grapevine samples from 12 regions”

✓ located verbatim in the source · abstract, characters 153–186 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

New Algerian GFkV sequence data were submitted to GenBank.

Taxon
grapevineVitist0
Interactor
Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
Outcome
new sequence data submitted to GenBanke0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e0

“identified new sequence data submitted to GenBank”

✓ located verbatim in the source · abstract, characters 681–730 · show in text ↑
t0

“grapevine samples from 12 regions”

✓ located verbatim in the source · abstract, characters 153–186 · show in text ↑
t1

“Grapevine Fleck Virus (GFkV)”

✓ located verbatim in the source · title, characters 14–42 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Italian and Algerian GFkV isolates were phylogenetically related.

Taxon
grapevineVitist0
Interactor
Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
Outcome
Italian and Algerian isolates are relatede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Phylogenetic analysis revealed that the Italian and Algerian isolates are related”

✓ located verbatim in the source · abstract, characters 732–813 · show in text ↑
t0

“grapevine samples from 12 regions”

✓ located verbatim in the source · abstract, characters 153–186 · show in text ↑
t1

“Grapevine Fleck Virus (GFkV)”

✓ located verbatim in the source · title, characters 14–42 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors urged virus monitoring in national certification and plant health programs.

Taxon
grapevineVitist0
Interactor
Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
Outcome
integrating virus monitoring into national certification and plant health programse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e0

“integrating virus monitoring into national certification and plant health programs”

✓ located verbatim in the source · abstract, characters 859–941 · show in text ↑
t0

“grapevine samples from 12 regions”

✓ located verbatim in the source · abstract, characters 153–186 · show in text ↑
t1

“Grapevine Fleck Virus (GFkV)”

✓ located verbatim in the source · title, characters 14–42 · show in text ↑