Abstract
In vitro ovule culture serves as an experimental platform for exploring the growth and development processes of cotton fibers. However, over the decades, research on the in vitro ovule culture of upland cotton has remained underdeveloped. In this study, ovules collected 2 days post-anthesis (2 DPA) from the upland cotton genetic standard line TM-1 were used to investigate the effects of carbon sources (glucose, fructose, sucrose), kinetin (KT), and glutamine (Gln) on ovule growth and observed fiber development in vitro. The results showed that the ovules grew more favorably on a medium supplemented with 0.05 M glucose, 0.02 M fructose, and their degradation products as carbon sources. Regarding the role of KT, it has a slight inhibitory effect on the development of cotton fiber in vitro at a lower concentration (0.1 mg/L). However, as the concentration increased (0.5 mg/L), its effect shifted to promotion. Additionally, Gln demonstrated the ability to enhance the characteristics of fiber fluffiness. In this study, the optimized formula for the in vitro ovule culture of upland cotton was established. This method provides an improved technical system for the in vitro ovule culture of upland cotton, holding great potential for fiber function genomics and seed bioengineering in cotton.
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Claims 7
Claim · model-written summary
Ovules grew more favorably on medium supplemented with 0.05 M glucose.
- Taxon
- upland cottont0
- Organ / stage
- ovulese0
- Treatment
- 0.05 M glucosee0
- Outcome
- ovules grewe0
- Direction
- more favorablye0
Not reportedComparator
Verbatim
e0“ovules grew more favorably on a medium supplemented with 0.05 M glucose, 0.02 M fructose, and their degradation products as carbon sources”
t0“upland cotton”
Claim · model-written summary
Ovules grew more favorably on medium supplemented with 0.02 M fructose.
- Taxon
- upland cottont0
- Organ / stage
- ovulese0
- Treatment
- 0.02 M fructosee0
- Outcome
- ovules grewe0
- Direction
- more favorablye0
Not reportedComparator
Verbatim
e0“ovules grew more favorably on a medium supplemented with 0.05 M glucose, 0.02 M fructose, and their degradation products as carbon sources”
t0“upland cotton”
Claim · model-written summary
Degradation products used as carbon sources supported more favorable ovule growth.
- Taxon
- upland cottont0
- Organ / stage
- ovulese0
- Treatment
- their degradation products as carbon sourcese0
- Outcome
- ovules grewe0
- Direction
- more favorablye0
Not reportedComparator
Verbatim
e0“ovules grew more favorably on a medium supplemented with 0.05 M glucose, 0.02 M fructose, and their degradation products as carbon sources”
t0“upland cotton”
Claim · model-written summary
Kinetin at 0.1 mg/L slightly inhibited cotton fiber development in vitro.
- Taxon
- cotton
Gossypium hirsutume0 - Treatment
- 0.1 mg/Le0
- Outcome
- development of cotton fiber in vitroe0
- Direction
- inhibitorye0
- Value
- 0.1 mg/Le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“slight inhibitory effect on the development of cotton fiber in vitro at a lower concentration (0.1 mg/L)”
Claim · model-written summary
Kinetin at 0.5 mg/L promoted cotton fiber development.
- Taxon
- upland cottont0
- Treatment
- KTe1
- Outcome
- development of cotton fiber in vitroe2
- Direction
- promotione0
- Value
- 0.5 mg/Le0
Not reportedComparator
Verbatim
e0“as the concentration increased (0.5 mg/L), its effect shifted to promotion”
e1“Regarding the role of KT”
e2“development of cotton fiber in vitro”
t0“upland cotton”
Claim · model-written summary
Glutamine improved fiber fluffiness.
Not reportedComparator
Verbatim
e0“Gln demonstrated the ability to enhance the characteristics of fiber fluffiness”
t0“upland cotton”
Claim · model-written summary
The study established an optimized in vitro ovule culture formula for upland cotton.
- Taxon
- upland cottone0
- Outcome
- optimized formula for the in vitro ovule culture of upland cotton was establishede0
Not reportedComparatorValue
Verbatim
e0“optimized formula for the in vitro ovule culture of upland cotton was established”
The same questions in other papers
kinetin · quality: grain · Gossypium hirsutum (cotton)Results differ
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.