Paper · 2025 · HortTechnology

Stratified Cutting Propagation Substrates Reduce Herbaceous Plug Structural Stability but Facilitate Similar Root Growth to a Control

Benjamin K. Hoover; Martin J. De La Cruz; Emma C. Whitehead; et al.

9
claims
17
verbatim quotes
0
citations
1
research areas
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Open access · cc-by-nc-nd

Abstract

Adventitious root development on cuttings may be promoted by oxygen availability in the rooting zone. Commercial propagation substrates are frequently peat-based, which can be problematic when used in propagation systems with intermittent misting due to excessive water holding. We tested top- or middle-stratifying a peat-based substrate with coarse amendments or a fine biochar. We had seven treatments, with a commercial peat-based propagation substrate as our control, then applying either a top (3 cm deep) or middle (2 cm deep) layer of coarse perlite, parboiled rice hulls, or fine coconut shell biochar in a pot with a top diameter of 6.3 cm, depth of 6.1 cm, and volume of 140 cm 3 . We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)]. We used completely randomized designs, with each plant species treated as a separate experiment. Each experiment had a replication of 7. We repeated the experiments in two series. After ∼4 weeks, we evaluated plug stability with a subjective scale (0 = no visible roots, unstable; 1 = minimal roots, one or two roots visible, unstable; 2 = moderate roots, more than two roots but large spaces with no roots, partially unstable; 3 = acceptable roots, many roots with only small gaps, stable; 4 = optimum roots, many roots with full coverage, stable), dry root weight, total root area, and total root length. Stratification significantly reduced plug structural stability. Across experiments and series, there was not a clear stratification effect on root growth. Considering that stratification with perlite, parboiled rice hulls, and fine biochar reduced plug structural stability but did not provide clear growth advantages, growers should consider alternate stratification materials that provide sufficient binding to maintain plug stability.

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Claims 9

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Stratification significantly reduced plug stability for hybrid hyssop.

Taxon
hybrid hyssopDracocephalum officinalee1
Treatment
Stratificatione0
Outcome
plug structural stabilitye0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dracocephalum officinale
Dracocephalum officinale · TaxonRobert Flogaus-Faust · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stratification significantly reduced plug structural stability.”

✓ located verbatim in the source · abstract, characters 1562–1625 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Stratification had no clear effect on root growth for hybrid hyssop.

Taxon
hybrid hyssopDracocephalum officinalee1
Treatment
stratificatione0
Outcome
root growthe0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dracocephalum officinale
Dracocephalum officinale · TaxonRobert Flogaus-Faust · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Across experiments and series, there was not a clear stratification effect on root growth.”

✓ located verbatim in the source · abstract, characters 1626–1716 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Stratification significantly reduced plug stability for false heather.

Taxon
false heathere1
Treatment
Stratificatione0
Outcome
plug structural stabilitye0
Direction
reducede0

Not reportedComparator

Verbatim

e0

“Stratification significantly reduced plug structural stability.”

✓ located verbatim in the source · abstract, characters 1562–1625 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Stratification had no clear effect on root growth for false heather.

Taxon
false heathere1
Treatment
stratificatione0
Outcome
root growthe0

Not reportedComparatorDirection

Verbatim

e0

“Across experiments and series, there was not a clear stratification effect on root growth.”

✓ located verbatim in the source · abstract, characters 1626–1716 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Stratification significantly reduced plug stability for blanket flower.

Taxon
blanket flowerGaillardia aristatae1
Treatment
Stratificatione0
Outcome
plug structural stabilitye0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gaillardia aristata
Gaillardia aristata · TaxonAlvesgaspar · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Stratification significantly reduced plug structural stability.”

✓ located verbatim in the source · abstract, characters 1562–1625 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Stratification had no clear effect on root growth for blanket flower.

Taxon
blanket flowerGaillardia aristatae1
Treatment
stratificatione0
Outcome
root growthe0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gaillardia aristata
Gaillardia aristata · TaxonAlvesgaspar · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Across experiments and series, there was not a clear stratification effect on root growth.”

✓ located verbatim in the source · abstract, characters 1626–1716 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Stratification significantly reduced plug stability for Jame sage.

Taxon
Jame sagee1
Treatment
Stratificatione0
Outcome
plug structural stabilitye0
Direction
reducede0

Not reportedComparator

Verbatim

e0

“Stratification significantly reduced plug structural stability.”

✓ located verbatim in the source · abstract, characters 1562–1625 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Stratification had no clear effect on root growth for Jame sage.

Taxon
Jame sagee1
Treatment
stratificatione0
Outcome
root growthe0

Not reportedComparatorDirection

Verbatim

e0

“Across experiments and series, there was not a clear stratification effect on root growth.”

✓ located verbatim in the source · abstract, characters 1626–1716 · show in text ↑
e1

“We stuck unrooted cuttings of four herbaceous plants [hybrid hyssop ( Agastache × ‘Sunrise Red Improved’), false heather ( Cuphea hyssopifolia ‘Maria’), blanket flower ( Gaillardia × grandiflora ‘Eclipse’), and Jame sage ( Salvia × jamensis ‘Ignition Purple’)].”

✓ located verbatim in the source · abstract, characters 693–954 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Growers should consider alternate stratification materials that bind well enough to maintain plug stability.

Taxon
growerse0
Outcome
alternate stratification materials that provide sufficient binding to maintain plug stabilitye0

Verbatim

e0

“Considering that stratification with perlite, parboiled rice hulls, and fine biochar reduced plug structural stability but did not provide clear growth advantages, growers should consider alternate stratification materials that provide sufficient binding to maintain plug stability.”

✓ located verbatim in the source · abstract, characters 1717–1999 · show in text ↑