Abstract
ABSTRACT Vibrio is an important marine heterotroph, primarily studied for its pathogenesis or symbiotic relationship with marine organisms and humans. However, little is known about the association of vibrios with plants in brackish environments and their potential benefits. To address this knowledge gap, we focused on Vibrio porteresiae MSSRF30 T and brackish-grown Pokkali rice as our research subjects for this study. MSSRF30 T displays multifaceted plant beneficial traits, including nitrogen fixation, 1-aminocyclopropane-1-carboxylate (ACC) deaminase production, and zinc and tricalcium phosphate solubilization. Further, MSSRF30 T efficiently colonizes the host roots and significantly improves the Pokkali rice growth in nitrogen-replete and nitrogen-limiting brackish conditions, highlighting its plant growth-promoting ability, a trait previously not well recognized in vibrios. Additionally, MSSRF30 T can utilize various carbon-rich substrates derived from plant roots, demonstrating its metabolic adaptation to the plant rhizosphere niche. Using in planta root transcriptome analysis and whole-genome sequencing, we provide the first insights into how MSSRF30 T interacts with Pokkali rice in brackish conditions. Additionally, we have identified several genome features for a plant-associated lifestyle, previously unreported in this genus. These features include plant expansin, PEP-CTERM surface anchoring with exopolysaccharides, plant-associated Hrp-type three secretion system, ACC deaminase production, PQQ-independent glucose dehydrogenase pathway for phosphate solubilization, plant-derived sugar/organic acids utilization operons, carbohydrate utilization loci, and specific plant depolymerizing CAZymes. Notably, MSSRF30 T lacks key genome features critical for the animal association. Overall, this study adds new knowledge in the field of Vibrio biology, especially Vibrio -plant beneficial interactions, a relationship largely underexplored. IMPORTANCE The genus Vibrio comprises over 150 species of marine heterotrophic bacteria, many of which are opportunistic pathogens affecting humans and marine animals. Most research has predominantly focused on pathogenic Vibrio species, often overlooking the significance of other Vibrio species inhabiting other ecological niches, such as plants, a relationship largely uncharacterized. This study focused on V. porteresiae MSSRF30 T and its relationship with brackish-grown Pokkali rice. We discovered that MSSRF30 T possesses multiple plant growth-promoting traits, effectively colonizes roots, and enhances plant growth in brackish conditions. Additionally, MSSRF30 T possesses several genome features commonly associated with plant-microbe interactions, previously unrecognized in Vibrio species, and lacks features typically associated with animal interactions, underscoring its specialized adaptation for plant niches. For the first time, this study highlights the beneficial interactions between Vibrio and plants, emphasizing their role in promoting plant growth and health in brackish environments.
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Claims 5
Claim · model-written summary
Vibrio porteresiae MSSRF30 T fixes nitrogen, produces ACC deaminase and solubilizes zinc and tricalcium phosphate.
- Taxon
- Vibrio porteresiae MSSRF30 TVibrio porteresiaee1
- Outcome
- nitrogen fixation, 1-aminocyclopropane-1-carboxylate (ACC) deaminase production, and zinc and tricalcium phosphate solubilizatione0
Verbatim
e0“MSSRF30 T displays multifaceted plant beneficial traits, including nitrogen fixation, 1-aminocyclopropane-1-carboxylate (ACC) deaminase production, and zinc and tricalcium phosphate solubilization.”
e1“Vibrio porteresiae MSSRF30 T”
Claim · model-written summary
MSSRF30 T significantly improves Pokkali rice growth in nitrogen-replete and nitrogen-limiting brackish conditions.
- Taxon
- Pokkali ricee0
- Treatment
- MSSRF30 Te0
- Interactor
- MSSRF30 Te0symbiont
- Outcome
- Pokkali rice growthe0
- Direction
- improvese0
- Qualifier
- nitrogen-replete and nitrogen-limiting brackish conditionse0
Not reportedComparator
Verbatim
e0“MSSRF30 T efficiently colonizes the host roots and significantly improves the Pokkali rice growth in nitrogen-replete and nitrogen-limiting brackish conditions”
Claim · model-written summary
MSSRF30 T utilizes diverse carbon-rich plant-root substrates.
- Taxon
- Vibrio porteresiae MSSRF30 TVibrio porteresiaee1
- Outcome
- utilize various carbon-rich substrates derived from plant rootse0
Verbatim
e0“MSSRF30 T can utilize various carbon-rich substrates derived from plant roots”
e1“Vibrio porteresiae MSSRF30 T”
Claim · model-written summary
Plant-associated genome features were identified in MSSRF30 T, including expansin, secretion and metabolic pathways.
- Taxon
- Vibrio porteresiae MSSRF30 TVibrio porteresiaee1
- Outcome
- genome features for a plant-associated lifestylee0
Verbatim
e0“we have identified several genome features for a plant-associated lifestyle, previously unreported in this genus. These features include plant expansin, PEP-CTERM surface anchoring with exopolysaccharides, plant-associated Hrp-type three secretion system, ACC deaminase production, PQQ-independent glucose dehydrogenase pathway for phosphate solubilization, plant-derived sugar/organic acids utilization operons, carbohydrate utilization loci, and specific plant depolymerizing CAZymes.”
e1“Vibrio porteresiae MSSRF30 T”
Claim · model-written summary
MSSRF30 T lacks key genome features for animal association.
- Taxon
- Vibrio porteresiae MSSRF30 TVibrio porteresiaee1
- Outcome
- key genome features critical for the animal associatione0
Verbatim
e0“MSSRF30 T lacks key genome features critical for the animal association.”
e1“Vibrio porteresiae MSSRF30 T”