Abstract
Bean yellow mosaic potyvirus (BYMV) is a highly destructive virus that inflicts significant economic damage on many important plants worldwide. It naturally infects numerous species of legumes as well as some non-leguminous plants, such as canna plants. The current study aimed to isolate and identify BYMV from naturally infected Canna plants and to produce BYMV polyclonal antibodies. The naturally infected Canna plants exhibiting typical streak mosaic patterns on the infected leaves, along with black spots grown in nurseries in the Alexandria governorate of Egypt, were collected. The BYMV virus was detected in infected Canna leaves sap by indirect ELISA and confirmed by RT-PCR via Cp-gene with an expected size of 525bp. The nucleotide sequence of BYMV-EG2 Cp-gene isolate recorded in NCBI under (Acc. No. PQ836641.1). BYMV isolate was transmitted in a non-persistent manner by Acyrthosiphon pisum, Aphis fabae, A. nerii and Brevicoryne brassicae, with average transmission rates of 66.7, 75, 33.3, and 50 % respectively. The partially purified virus using polyethylene glycol (PEG) 6000 and ultra-centrifugation have purity A260/280 ratio was 1.17, and the Amax/min ratio was 0.85. The virus yield was 13.8 mg/100 g of fresh weight infected faba bean leaves. The immunogenicity of the virus isolate produced an antiserum titer was to 1:1.28x105 determined by indirect ELISA. The transmission electron microscopy revealed that circular inclusions were formed by the extension of pinwheel circles in Canna leaves. The TEM highlighted flexible rod-shaped particles of BYMV measuring approximately 740 nm in length by the negatively stained method with phosphotungstic acid.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
BYMV was detected in infected Canna leaf sap by indirect ELISA and confirmed by RT-PCR
- Interactor
- BYMV
Bean yellow mosaic viruse1pathogen - Outcome
- The BYMV virus was detectede1
CannaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e1“The BYMV virus was detected in infected Canna leaves sap by indirect ELISA and confirmed by RT-PCR via Cp-gene with an expected size of 525bp”
Claim · model-written summary
BYMV was transmitted in a non-persistent manner by four aphid species
- Interactor
- BYMVBean yellow mosaic viruse2pathogen
- Second interactor
- Acyrthosiphon pisum
-PLoS.jpg?width=60)
Acyrthosiphon pisume2pest - Outcome
- transmitted in a non-persistent mannere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
-PLoS.jpg?width=500)
Verbatim
t0“Canna Plants”
e2“BYMV isolate was transmitted in a non-persistent manner by Acyrthosiphon pisum, Aphis fabae, A. nerii and Brevicoryne brassicae, with average transmission rates of 66.7, 75, 33.3, and 50 % respectively”
Claim · model-written summary
Acyrthosiphon pisum transmitted BYMV at an average rate of 66.7%
- Interactor
- BYMVBean yellow mosaic viruse2pathogen
- Second interactor
- Acyrthosiphon pisumAcyrthosiphon pisume2pest
- Outcome
- average transmission ratese2
- Value
- 66.7e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e2“BYMV isolate was transmitted in a non-persistent manner by Acyrthosiphon pisum, Aphis fabae, A. nerii and Brevicoryne brassicae, with average transmission rates of 66.7, 75, 33.3, and 50 % respectively”
Claim · model-written summary
Aphis fabae transmitted BYMV at an average rate of 75%
- Interactor
- BYMVBean yellow mosaic viruse2pathogen
- Second interactor
- Aphis fabae
Aphis fabaee2pest - Outcome
- average transmission ratese2
- Value
- 75e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e2“BYMV isolate was transmitted in a non-persistent manner by Acyrthosiphon pisum, Aphis fabae, A. nerii and Brevicoryne brassicae, with average transmission rates of 66.7, 75, 33.3, and 50 % respectively”
Claim · model-written summary
Aphis nerii transmitted BYMV at an average rate of 33.3%
- Interactor
- BYMVBean yellow mosaic viruse2pathogen
- Second interactor
- A. nerii
Aphis neriie2pest - Outcome
- average transmission ratese2
- Value
- 33.3e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e2“BYMV isolate was transmitted in a non-persistent manner by Acyrthosiphon pisum, Aphis fabae, A. nerii and Brevicoryne brassicae, with average transmission rates of 66.7, 75, 33.3, and 50 % respectively”
Claim · model-written summary
Brevicoryne brassicae transmitted BYMV at an average rate of 50%
- Interactor
- BYMVBean yellow mosaic viruse2pathogen
- Second interactor
- Brevicoryne brassicae
.jpg?width=60)
Brevicoryne brassicaee2pest - Outcome
- average transmission ratese2
- Value
- 50e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Canna Plants”
e2“BYMV isolate was transmitted in a non-persistent manner by Acyrthosiphon pisum, Aphis fabae, A. nerii and Brevicoryne brassicae, with average transmission rates of 66.7, 75, 33.3, and 50 % respectively”
Claim · model-written summary
BYMV yield from infected faba bean leaves was 13.8 mg/100 g fresh weight
- Taxon
- faba bean
Vicia fabae3 - Organ / stage
- leavese3
- Outcome
- virus yielde3
- Value
- 13.8 mg/100 ge3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The virus yield was 13.8 mg/100 g of fresh weight infected faba bean leaves”
Claim · model-written summary
Partially purified BYMV had a purity A260/280 ratio of 1.17
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e4“The partially purified virus using polyethylene glycol (PEG) 6000 and ultra-centrifugation have purity A260/280 ratio was 1.17, and the Amax/min ratio was 0.85”
Claim · model-written summary
Partially purified BYMV had an Amax/min ratio of 0.85
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e4“The partially purified virus using polyethylene glycol (PEG) 6000 and ultra-centrifugation have purity A260/280 ratio was 1.17, and the Amax/min ratio was 0.85”
Claim · model-written summary
The BYMV isolate produced an antiserum with an indirect ELISA titer of 1:1.28x105
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e5“The immunogenicity of the virus isolate produced an antiserum titer was to 1:1.28x105 determined by indirect ELISA”
Claim · model-written summary
Circular inclusions were formed by the extension of pinwheel circles in Canna leaves
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The transmission electron microscopy revealed that circular inclusions were formed by the extension of pinwheel circles in Canna leaves”
Claim · model-written summary
BYMV rod-shaped particles measured approximately 740 nm in length by TEM
- Interactor
- BYMVBean yellow mosaic viruse7pathogen
- Outcome
- flexible rod-shaped particlese7
- Value
- approximately 740 nme7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna Plants”
e7“The TEM highlighted flexible rod-shaped particles of BYMV measuring approximately 740 nm in length”