Abstract
This dataset presents the first transcriptome for Phlebodium aureum ‘Mandaianum’, a naturally cyanogenic fern known for producing an (R)-selective hydroxynitrile lyase (HNL) enzyme. Total RNA was extracted from frond tissue and sequenced using the Illumina HiSeq 2500 platform. Following quality control processes, the reads were assembled de novo into 76 562 transcripts. The transcripts had a mean sequence length of 954 bp and an N50 of 1 427 bp. BUSCO analysis showed that 76.5% of genes were complete, 16.5% were fragmented, and 7% were missing. The translated transcriptome was then annotated with COG, GO, EC, KEGG and Pfam using eggNOG Mapper. This resource will support the identification of the HNL gene for heterologous expression, the investigation of genes involved in secondary metabolite biosynthesis, and the exploration of metabolic pathways in ferns. It also serves as a reference for comparative transcriptomics, evolutionary studies, and functional genomics in related plant taxa.
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Claims 8
Claim · model-written summary
The first transcriptome resource for the fern was presented.
- Taxon
- Phlebodium aureum ‘Mandaianum’
Phlebodium aureume0 - Outcome
- first transcriptomee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This dataset presents the first transcriptome for Phlebodium aureum ‘Mandaianum’”
Claim · model-written summary
The assembly contained 76 562 transcripts.
- Taxon
- Phlebodium aureum ‘Mandaianum’Phlebodium aureumt0
- Outcome
- transcriptse0
- Value
- 76 562 transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reads were assembled de novo into 76 562 transcripts.”
t0“Phlebodium aureum ‘Mandaianum’”
Claim · model-written summary
Mean transcript length was 954 bp.
- Taxon
- Phlebodium aureum ‘Mandaianum’Phlebodium aureumt0
- Outcome
- mean sequence lengthe0
- Value
- 954 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcripts had a mean sequence length of 954 bp”
t0“Phlebodium aureum ‘Mandaianum’”
Claim · model-written summary
Assembly N50 was 1 427 bp.
- Taxon
- Phlebodium aureum ‘Mandaianum’Phlebodium aureumt0
- Outcome
- N50e0
- Value
- 1 427 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an N50 of 1 427 bp.”
t0“Phlebodium aureum ‘Mandaianum’”
Claim · model-written summary
BUSCO found 76.5% complete genes.
- Taxon
- Phlebodium aureum ‘Mandaianum’Phlebodium aureumt0
- Outcome
- genes were completee0
- Value
- 76.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“76.5% of genes were complete”
t0“Phlebodium aureum ‘Mandaianum’”
Claim · model-written summary
BUSCO found 16.5% fragmented genes.
- Taxon
- Phlebodium aureum ‘Mandaianum’Phlebodium aureumt0
- Outcome
- fragmentede0
- Value
- 16.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“16.5% were fragmented”
t0“Phlebodium aureum ‘Mandaianum’”
Claim · model-written summary
BUSCO found 7% missing genes.
- Taxon
- Phlebodium aureum ‘Mandaianum’Phlebodium aureumt0
- Outcome
- missinge0
- Value
- 7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7% were missing.”
t0“Phlebodium aureum ‘Mandaianum’”
Claim · model-written summary
The translated transcriptome was annotated with COG, GO, EC, KEGG, and Pfam.
- Taxon
- Phlebodium aureum ‘Mandaianum’Phlebodium aureumt0
- Outcome
- annotated with COG, GO, EC, KEGG and Pfame0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The translated transcriptome was then annotated with COG, GO, EC, KEGG and Pfam using eggNOG Mapper.”
t0“Phlebodium aureum ‘Mandaianum’”