A CiteSpace-based bibliometric analysis of research hotspots and trends in orchid tissue culture

Abstract

Research on orchid tissue culture and rapid propagation technologies provides key solutions for improving propagation efficiency, expanding seedling production, and cultivating new varieties. However, in the field of orchid tissue culture and rapid propagation research, there is a lack of comprehensive, quantitative analyses, and data-driven studies. The bibliometric software CiteSpace was used to conduct a visual quantitative analysis of 1,287 publications (January 1, 2000, to June 30, 2025) in the Web of Science (WOS) and China National Knowledge Infrastructure (CNKI) database to address this gap. The results indicate that, since 2020, research has increasingly focused on economically valuable orchids such as Dendrobium officinale, Phalaenopsis spp., and Cymbidium hybridum. Current research hotspots center on the regulatory roles of endophytic fungi in tissue culture and rapid propagation, as well as on the systematic optimization of culture-medium formulations; in parallel, preventive and corrective strategies to address persistent bottlenecks—including contamination, browning, and vitrification—are being continuously refined. In recent years, the field has increasingly shifted toward molecular-level inquiry, emphasizing elucidation of the genetic and regulatory mechanisms underlying key traits, including the formation of floral color and fragrance, floral organ morphogenesis (e.g., MADS-box gene networks), and abiotic stress tolerance (e.g., drought and heat). This study seeks to provide theoretical foundations and methodological references for advancing tissue culture and rapid propagation techniques in orchids.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.