Paper · 2026 · Basrah Journal of Agricultural Sciences

Comparative Analysis of Chloroplast Genome (rbcL Sequences) in Ten Date Palm Cultivars Propagated via Vegetative and Tissue Culture

Al-hamza A.M. Al-Salim; Aqeel A. Suhaim; Osama N. Jaffer

12
claims
24
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by-nc

Abstract

The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop. It is cornerstone of Middle Eastern agriculture, the date palm. Despite its economic importance, there is limited published genetic information on the plastid genomes of date palm. This study addresses a knowledge gap by investigating the chloroplast (cp) genomes of ten date palm cultivars that are economically important but haven't had their genomes studied before. These cultivars, propagated vegetatively and through tissue culture, were sequenced. The data annotation revealed similar gene annotations to those of other the palm family (Arecaceae), and the genome sizes of the tissue-cultured cultivars including Barhi (156.445 bp), Sag'ai (156.700 bp), Khalas (156.500 bp), Zamli (156.386 bp) and Maghrabi(156.386 bp) (. In addition, the genome sizes of the vegetatively propagated cultivars including Barhi (156.192 bp), Hassawi (156.2018 bp), Berim (156.523 bp), Dairi (156.129 bp) and Shakar (156.517 bp). Structurally, the phylogenetic analysis based on the complete chloroplast genomes showed a consistent phylogenetic grouping among all cultivars with a similarity of 97.8% compared to global cultivars. The current study presents a comparative analysis of the chloroplast genome by registering sequences for the rbcL gene with the National Center for Biotechnology Information (NCBI). The results are vital for expanding research into population genetics and the molecular biology of date palm cultivars.

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Claims 12

Observationobservedoriginal resultmeasured

Claim · model-written summary

Tissue-cultured Barhi had a genome size of 156.445 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Barhie1
Treatment
tissue-culturede1
Outcome
genome sizee1
Value
156.445 bpe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e1

“the genome sizes of the tissue-cultured cultivars including Barhi (156.445 bp), Sag'ai (156.700 bp), Khalas (156.500 bp), Zamli (156.386 bp) and Maghrabi(156.386 bp)”

✓ located verbatim in the source · abstract, characters 645–810 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tissue-cultured Sag'ai had a genome size of 156.700 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Sag'aie1
Treatment
tissue-culturede1
Outcome
genome sizee1
Value
156.700 bpe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e1

“the genome sizes of the tissue-cultured cultivars including Barhi (156.445 bp), Sag'ai (156.700 bp), Khalas (156.500 bp), Zamli (156.386 bp) and Maghrabi(156.386 bp)”

✓ located verbatim in the source · abstract, characters 645–810 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tissue-cultured Khalas had a genome size of 156.500 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Khalase1
Treatment
tissue-culturede1
Outcome
genome sizee1
Value
156.500 bpe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e1

“the genome sizes of the tissue-cultured cultivars including Barhi (156.445 bp), Sag'ai (156.700 bp), Khalas (156.500 bp), Zamli (156.386 bp) and Maghrabi(156.386 bp)”

✓ located verbatim in the source · abstract, characters 645–810 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tissue-cultured Zamli had a genome size of 156.386 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Zamlie1
Treatment
tissue-culturede1
Outcome
genome sizee1
Value
156.386 bpe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e1

“the genome sizes of the tissue-cultured cultivars including Barhi (156.445 bp), Sag'ai (156.700 bp), Khalas (156.500 bp), Zamli (156.386 bp) and Maghrabi(156.386 bp)”

✓ located verbatim in the source · abstract, characters 645–810 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Tissue-cultured Maghrabi had a genome size of 156.386 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Maghrabie1
Treatment
tissue-culturede1
Outcome
genome sizee1
Value
156.386 bpe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e1

“the genome sizes of the tissue-cultured cultivars including Barhi (156.445 bp), Sag'ai (156.700 bp), Khalas (156.500 bp), Zamli (156.386 bp) and Maghrabi(156.386 bp)”

✓ located verbatim in the source · abstract, characters 645–810 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vegetatively propagated Barhi had a genome size of 156.192 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Barhie2
Treatment
vegetatively propagatede2
Outcome
genome sizee2
Value
156.192 bpe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e2

“the genome sizes of the vegetatively propagated cultivars including Barhi (156.192 bp), Hassawi (156.2018 bp), Berim (156.523 bp), Dairi (156.129 bp) and Shakar (156.517 bp)”

✓ located verbatim in the source · abstract, characters 827–1000 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vegetatively propagated Hassawi had a genome size of 156.2018 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Hassawie2
Treatment
vegetatively propagatede2
Outcome
genome sizee2
Value
156.2018 bpe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e2

“the genome sizes of the vegetatively propagated cultivars including Barhi (156.192 bp), Hassawi (156.2018 bp), Berim (156.523 bp), Dairi (156.129 bp) and Shakar (156.517 bp)”

✓ located verbatim in the source · abstract, characters 827–1000 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vegetatively propagated Berim had a genome size of 156.523 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Berime2
Treatment
vegetatively propagatede2
Outcome
genome sizee2
Value
156.523 bpe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e2

“the genome sizes of the vegetatively propagated cultivars including Barhi (156.192 bp), Hassawi (156.2018 bp), Berim (156.523 bp), Dairi (156.129 bp) and Shakar (156.517 bp)”

✓ located verbatim in the source · abstract, characters 827–1000 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vegetatively propagated Dairi had a genome size of 156.129 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Dairie2
Treatment
vegetatively propagatede2
Outcome
genome sizee2
Value
156.129 bpe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e2

“the genome sizes of the vegetatively propagated cultivars including Barhi (156.192 bp), Hassawi (156.2018 bp), Berim (156.523 bp), Dairi (156.129 bp) and Shakar (156.517 bp)”

✓ located verbatim in the source · abstract, characters 827–1000 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Vegetatively propagated Shakar had a genome size of 156.517 bp.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Material
Shakare2
Treatment
vegetatively propagatede2
Outcome
genome sizee2
Value
156.517 bpe2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e2

“the genome sizes of the vegetatively propagated cultivars including Barhi (156.192 bp), Hassawi (156.2018 bp), Berim (156.523 bp), Dairi (156.129 bp) and Shakar (156.517 bp)”

✓ located verbatim in the source · abstract, characters 827–1000 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Phylogenetic analysis of complete chloroplast genomes grouped the cultivars consistently, with 97.8% similarity compared to global cultivars.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Outcome
phylogenetic grouping among all cultivarse3
Value
97.8%e3
Comparator
global cultivarse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e3

“the phylogenetic analysis based on the complete chloroplast genomes showed a consistent phylogenetic grouping among all cultivars with a similarity of 97.8% compared to global cultivars”

✓ located verbatim in the source · abstract, characters 1016–1201 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Gene annotations of the date palm chloroplast genomes were similar to those of other palm family (Arecaceae) members.

Taxon
Phoenix dactyliferaPhoenix dactyliferae0
Outcome
gene annotationse4
Comparator
other the palm family (Arecaceae)e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e4

“The data annotation revealed similar gene annotations to those of other the palm family (Arecaceae)”

✓ located verbatim in the source · abstract, characters 540–639 · show in text ↑
e0

“The date palm Phoenix dactylifera L. is recognized as a vitally important fruit crop”

✓ located verbatim in the source · abstract, characters 0–84 · show in text ↑