Abstract
Canadian spring wheat cultivars AAC Concord and Lillian possess Lr30, a gene that confers resistance against leaf rust caused by Puccinia triticina Eriks. (Pt). The objectives of this study were to generate the first linkage map of Lr30, and develop Kompetitive allele-specific PCR (KASP) markers linked to Lr30 for marker-assisted breeding. Two doubled haploid (DH) populations were generated from the crosses Lillian × Glenlea (LG) and CDC Hughes × AAC Concord (HC). The LG population was phenotyped for seedling leaf rust reaction using the Lr30 avirulent Pt isolates 96–12-3 MBDS, 74–2 MGBJ, 06–1-1 TDBG, and 77–2 TJBJ, and both populations were phenotyped with Pt isolate 21–21-1 TNBJ. The 90k iSelect SNP array was used to genotype the populations for linkage mapping. The LG population showed a 1:1 resistant to susceptible monogenic segregation ratio for all five Pt isolates, where the resistance to each race co-segregated. The HC population also showed a 1:1 phenotypic ratio when inoculated with Pt isolate 21–21-1 TNBJ. Lr30 was localized to genetic intervals of 2.7 cM and 1.6 cM on chromosome 4A of the LG and HC linkage maps, respectively. The physical location of Lr30 in the Chinese Spring wheat RefSeq.V.2.1 spans approximately between positions 522.0 Mb and 548.0 Mb of chromosome 4A. KASP markers were designed and validated for functionality using the LG and HC populations, backcross lines of wheat cultivar Pasqua with Thatcher, and a panel of 193 wheat cultivars. These new KASP markers are well-suited to marker-assisted breeding.
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Claims 8
Claim · model-written summary
The LG wheat population showed 1:1 resistant-to-susceptible monogenic segregation for all five isolates.
- Taxon
- wheat
Triticum aestivumt0 - Material
- LG populatione0
- Interactor
- Pte0pathogen
- Outcome
- resistant to susceptible monogenic segregation ratioe0
- Value
- 1:1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LG population showed a 1:1 resistant to susceptible monogenic segregation ratio for all five Pt isolates, where the resistance to each race co-segregated.”
t0“wheat”
Claim · model-written summary
Resistance to each race co-segregated in LG wheat.
- Taxon
- wheatTriticum aestivumt0
- Material
- LG populatione0
- Interactor
- Pte0pathogen
- Outcome
- resistance to each race co-segregatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LG population showed a 1:1 resistant to susceptible monogenic segregation ratio for all five Pt isolates, where the resistance to each race co-segregated.”
t0“wheat”
Claim · model-written summary
HC wheat showed a 1:1 phenotypic ratio after inoculation with Pt isolate 21–21-1 TNBJ.
- Taxon
- wheatTriticum aestivumt0
- Material
- HC populatione0
- Treatment
- inoculated with Pt isolate 21–21-1 TNBJe0
- Interactor
- Pt isolate 21–21-1 TNBJe0pathogen
- Outcome
- phenotypic ratioe0
- Value
- 1:1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The HC population also showed a 1:1 phenotypic ratio when inoculated with Pt isolate 21–21-1 TNBJ.”
t0“wheat”
Claim · model-written summary
Lr30 mapped to a 2.7 cM interval on chromosome 4A in LG wheat.
- Taxon
- wheatTriticum aestivumt0
- Material
- LGe0
- Gene
- Lr30e0
- Outcome
- genetic intervalse0
- Value
- 2.7 cMe0
- Qualifier
- chromosome 4Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lr30 was localized to genetic intervals of 2.7 cM and 1.6 cM on chromosome 4A of the LG and HC linkage maps, respectively.”
t0“wheat”
Claim · model-written summary
Lr30 mapped to a 1.6 cM interval on chromosome 4A in HC wheat.
- Taxon
- wheatTriticum aestivumt0
- Material
- HCe0
- Gene
- Lr30e0
- Outcome
- genetic intervalse0
- Value
- 1.6 cMe0
- Qualifier
- chromosome 4Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lr30 was localized to genetic intervals of 2.7 cM and 1.6 cM on chromosome 4A of the LG and HC linkage maps, respectively.”
t0“wheat”
Claim · model-written summary
Lr30 physically spans approximately 522.0–548.0 Mb of chromosome 4A in Chinese Spring wheat RefSeq.V.2.1.
- Taxon
- wheatTriticum aestivume0
- Material
- Chinese Springe0
- Gene
- Lr30e0
- Outcome
- physical locatione0
- Value
- 522.0 Mb and 548.0 Mbe0
- Qualifier
- approximatelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The physical location of Lr30 in the Chinese Spring wheat RefSeq.V.2.1 spans approximately between positions 522.0 Mb and 548.0 Mb of chromosome 4A.”
Claim · model-written summary
KASP markers were designed and functionally validated in populations, backcross lines and a cultivar panel.
- Taxon
- wheatTriticum aestivume0
- Outcome
- validated for functionalitye0
- Value
- 193 wheat cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KASP markers were designed and validated for functionality using the LG and HC populations, backcross lines of wheat cultivar Pasqua with Thatcher, and a panel of 193 wheat cultivars.”
Claim · model-written summary
The new KASP markers are well-suited to marker-assisted breeding.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- marker-assisted breedinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These new KASP markers are well-suited to marker-assisted breeding.”
t0“wheat”