Paper · 2026 · Journal of Food Composition and Analysis

HPLC-PDA method for simultaneous quantification of 37 phenolic compounds: Method validation and analysis of 19 plant samples

Zhihao Yang; Tianle Shen; Jinhu Tian; et al.

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Open access · cc-by-nc-nd

Abstract

The determination of phenolic compounds serves as a critical approach for food quality control and screening of bioactive phytochemicals. This study developed a high-performance liquid chromatography-photodiode array detection (HPLC-PDA)-based analytical technique for plant phenolics, enabling simultaneous accurate quantification of 37 representative phenolic compounds in samples. Method validation demonstrated excellent linearity, precision, and accuracy, with intra-day and inter-day precision below 3%, and spiked recoveries ranging from 96.88% to 103.75%. The methodology was successfully applied to analyze 19 distinct plant specimens, confirming its exceptional versatility. This method can be used as an efficient assay for plant polyphenols in multiple fields including food analysis, phytochemical research, and bioactive component development.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

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Claims 5

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

An HPLC-PDA method was developed that simultaneously and accurately quantifies 37 representative phenolic compounds in plant samples.

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simultaneous accurate quantification of 37 representative phenolic compoundse0
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37e0

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e0

“developed a high-performance liquid chromatography-photodiode array detection (HPLC-PDA)-based analytical technique for plant phenolics, enabling simultaneous accurate quantification of 37 representative phenolic compounds in samples”

✓ located verbatim in the source · abstract, characters 149–382 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Method validation showed excellent linearity, precision and accuracy.

Outcome
linearity, precision, and accuracye0
Value
excellente0

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e0

“Method validation demonstrated excellent linearity, precision, and accuracy”

✓ located verbatim in the source · abstract, characters 384–459 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Intra-day and inter-day precision were below 3%.

Outcome
intra-day and inter-day precisione0
Value
below 3%e0

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e0

“intra-day and inter-day precision below 3%”

✓ located verbatim in the source · abstract, characters 466–508 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Spiked recoveries ranged from 96.88% to 103.75%.

Outcome
spiked recoveriese0
Value
96.88% to 103.75%e0

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e0

“spiked recoveries ranging from 96.88% to 103.75%”

✓ located verbatim in the source · abstract, characters 514–562 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The method was successfully applied to analyze 19 distinct plant specimens.

Outcome
applied to analyze 19 distinct plant specimense0
Value
19e0

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e0

“The methodology was successfully applied to analyze 19 distinct plant specimens”

✓ located verbatim in the source · abstract, characters 564–643 · show in text ↑