Abstract
The biological reduction of N2O, a potent greenhouse gas, is crucial for environmental sustainability. We developed an automated system for continuous N2O monitoring in the gas phase of a flask containing an anaerobic bradyrhizobial culture, and then examined the kinetic parameters of bacterial N2O reduction. The maximum reaction rate (Vmax) was approximately 61-fold higher for Bradyrhizobium ottawaense SG09 (1,471 nmol h–1 109 cells–1) than for B. diazoefficiens USDA110 (24 nmol h–1 109 cells–1). Our kinetics analysis confirmed that SG09 maintained higher N2O-reducing activity than USDA110 even at the atmospheric concentration of N2O (0.34 ppm).
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