Abstract
Panax notoginseng is highly susceptible to root rot during cultivation, severely affecting its production and quality. Phospholipases participate in plant immunity by producing free fatty acids and conjugated lipids that activate downstream signaling cascades. However, genome-wide identification of PnPL genes in P. notoginseng remains limited. A total of 72 PnPL genes were identified in P. notoginseng : 48 PnPLA genes, 9 PnPLC genes and 15 PnPLD genes. Transcriptome and qRT-PCR analyses between healthy and diseased plants (CK, RⅠ and RⅡ) revealed 13 differentially expressed genes from PnPL Gene Family, 11 of which belonged to the PnPLA genes superfamily. Notably, PnPLA1-8 exhibited sustained upregulation with worsening root rot. Further, RNA interference (RNAi) mediated silencing of PnPLA1-8 gene increased susceptibility to Fusarium oxysporum that the main pathogenic fungus in P. notoginseng , whereas overexpression of PnPLA1-8 gene in Nicotiana tabacum enhanced resistance to F. oxysporum . This study suggests that the PnPLA1-8 genes exhibit potential roles in resistance to F. oxysporum . • Comprehensive genome-wide identification of phospholipase genes in Panax notoginseng. • Functional analysis suggest that PnPLA1-8 plays a potential positive regulatory role in resistance to Fusarium oxysporum. • Upregulation of PnPLA1-8 correlates with increased α-linolenic acid and JA levels in infected root rot.
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Claims 6
Claim · model-written summary
A total of 72 phospholipase (PnPL) genes were identified in Panax notoginseng: 48 PnPLA, 9 PnPLC and 15 PnPLD genes.
- Taxon
- P. notoginseng
Panax notoginsenge0 - Outcome
- PnPL genes were identifiede0
- Value
- 72 PnPL genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Panax notoginseng”
e0“A total of 72 PnPL genes were identified in P. notoginseng : 48 PnPLA genes, 9 PnPLC genes and 15 PnPLD genes”
Claim · model-written summary
Transcriptome and qRT-PCR analyses of healthy and diseased plants revealed 13 differentially expressed PnPL genes, 11 of them PnPLA genes.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Outcome
- differentially expressed genese0
- Value
- 13 differentially expressed genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Panax notoginseng”
e0“revealed 13 differentially expressed genes from PnPL Gene Family, 11 of which belonged to the PnPLA genes superfamily”
Claim · model-written summary
PnPLA1-8 expression showed sustained upregulation as root rot worsened.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Gene
- PnPLA1-8e0
- Outcome
- sustained upregulatione0
- Direction
- upregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Panax notoginseng”
e0“PnPLA1-8 exhibited sustained upregulation with worsening root rot”
Claim · model-written summary
RNAi silencing of PnPLA1-8 in Panax notoginseng increased susceptibility to Fusarium oxysporum.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Treatment
- RNA interference (RNAi) mediated silencing of PnPLA1-8 genee0
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Gene
- PnPLA1-8e0
- Outcome
- susceptibility to Fusarium oxysporume0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
t0“Panax notoginseng”
e0“RNA interference (RNAi) mediated silencing of PnPLA1-8 gene increased susceptibility to Fusarium oxysporum”
Claim · model-written summary
Overexpression of PnPLA1-8 in Nicotiana tabacum enhanced resistance to F. oxysporum.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Treatment
- overexpression of PnPLA1-8 genee0
- Interactor
- F. oxysporumFusarium oxysporume0pathogen
- Gene
- PnPLA1-8e0
- Outcome
- resistance to F. oxysporume0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PnPLA1-8 gene in Nicotiana tabacum enhanced resistance to F. oxysporum”
Claim · model-written summary
Upregulation of PnPLA1-8 correlates with increased α-linolenic acid and jasmonic acid levels in root rot-infected roots.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Gene
- PnPLA1-8e0
- Outcome
- α-linolenic acid and JA levelse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Panax notoginseng”
e0“Upregulation of PnPLA1-8 correlates with increased α-linolenic acid and JA levels in infected root rot”