Abstract
The native isolates of facultative entomopathogenic nematodes (Metarhabditis amsactae, Metarhabditis rainai and Oscheius myriophilus) and insect parasitic nematode (Acrobeloides saeedi) were tested against larvae and pupae of Clostera cupreata (Lepidoptera: Notodontidae) under laboratory conditions to assess their virulence. The native isolates were obtained from soil samples collected from poplar growing regions of North-western India. Overall, native nematodes were found effective against both stages tested under laboratory conditions. The values obtained for lethal concentrations (LC50 and LC90) of the tested nematode species differed significantly. The LC50 values of the nematode species against the larval and pupal stages were 59.3 and 93.6 IJ/ml for M. rainai, 34.6 and 89.8 IJ/ml for A. saeedi, 25.2 and 71.8 IJ/ml for M. amsactae and 15.8 and 63.6 IJ/ml for O. myriophilus respectively. Results of the present investigation demonstrate the biocontrol potential of native EPNs and insect parasitic nematode isolates against C. cupreata. However, multi-location field trials need to be conducted to confirm their efficacy in IPM programme for C. cupreata.
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