Abstract
The ITS-DNA region of Synchytrium endobioticum (Schilb.) Pere., rhe causal agent of poraro wart disease, was used ro generate specific PCR primers for molecular diagnosis of rhe disease. DNA was extracted from wart galls of all S. endobioticum pathotypes (I, 2, 6, 18) currently occurring and used for official resting purposes in Germany. Using the universal ITS primer # 4 and the S. endobioticumspecific primer Kbrl, a PCR fragment of 543 bp was obtained from all four fungal pathocypes. DNA for PCR-based diagnosis of the fungus could be extracted easily from summer sori, however, we did nor succeed ro extract amplifiable DNA from resting sori found in contaminated soils. To circumvent rhis problem, zoospores emerging from resring sori were used for DNA exrracrion and indirect detection. PCR also allowed ro discriminate between weakly resistant and moderately susceptible responses of potaro culrivars in addition ro rhe routine visual inspection, since only alive summer sori of the weakly susceptible reacting poraro culrivars released quantities of zoospores sufficient ro be detected. No PCR signal was obtained from weakly or completely resistant potaro cultivars.
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Claims 5
Claim · model-written summary
PCR yielded a 543 bp fragment from all four fungal pathotypes.
Verbatim
e0“a PCR fragment of 543 bp was obtained from all four fungal pathocypes”
e1“PCR also allowed ro discriminate between weakly resistant and moderately susceptible responses of potaro culrivars”
Claim · model-written summary
DNA was readily extracted from summer sori.
- Taxon
- Synchytrium endobioticum
Synchytrium endobioticume1 - Organ / stage
- summer sorie0
- Outcome
- DNA for PCR-based diagnosise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA for PCR-based diagnosis of the fungus could be extracted easily from summer sori”
e1“Synchytrium endobioticum (Schilb.) Pere.”
Claim · model-written summary
Amplifiable DNA could not be extracted from resting sori in contaminated soil.
- Taxon
- Synchytrium endobioticumSynchytrium endobioticume1
- Organ / stage
- resting sorie0
- Outcome
- amplifiable DNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we did nor succeed ro extract amplifiable DNA from resting sori found in contaminated soils”
e1“Synchytrium endobioticum (Schilb.) Pere.”
Claim · model-written summary
Zoospores emerging from resting sori enabled indirect DNA detection.
Verbatim
e0“zoospores emerging from resring sori were used for DNA exrracrion and indirect detection”
e1“PCR also allowed ro discriminate between weakly resistant and moderately susceptible responses of potaro culrivars”
Claim · model-written summary
PCR distinguished weakly resistant from moderately susceptible potato cultivar responses.
Verbatim
e0“PCR also allowed ro discriminate between weakly resistant and moderately susceptible responses of potaro culrivars”