Paper · 2026 · Plant Communications

Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata

Juan Li; Zenghui Chen; Kui Li; et al.

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claims
10
verbatim quotes
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citations
2
research areas
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Open access · cc-by

Abstract

families, with 15.5% showing visible phenotypic variation. This library provides a valuable resource for dissecting agronomic traits and supports forward genetic approaches for identifying key genes in C. moschata. Using this T2T genome assembly, we successfully identified the causal genes Cmos16G0077000 linked to a yellow-leaf phenotype and Cmos14G0126400 associated with a miniature squash (mSq) phenotype. Overall, the PKUMo T2T genome assembly, together with the extensive mutant library, provides a robust foundation for exploring agronomic traits and accelerating genetic improvement in Cucurbita breeding programs.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Associationobservedoriginal resultmeasured

Claim · model-written summary

Cmos16G0077000 was identified as a causal gene linked to the yellow-leaf phenotype.

Taxon
Cucurbita moschataCucurbita moschatat0
Gene
Cmos16G0077000e0
Outcome
yellow-leaf phenotypee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucurbita moschata”

✓ located verbatim in the source · title, characters 113–131 · show in text ↑
e0

“Using this T2T genome assembly, we successfully identified the causal genes Cmos16G0077000 linked to a yellow-leaf phenotype and Cmos14G0126400 associated with a miniature squash (mSq) phenotype”

✓ located verbatim in the source · abstract, characters 215–409 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Cmos14G0126400 was identified as a causal gene associated with the miniature squash (mSq) phenotype.

Taxon
Cucurbita moschataCucurbita moschatat0
Gene
Cmos14G0126400e0
Outcome
miniature squash (mSq) phenotypee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucurbita moschata”

✓ located verbatim in the source · title, characters 113–131 · show in text ↑
e0

“Using this T2T genome assembly, we successfully identified the causal genes Cmos16G0077000 linked to a yellow-leaf phenotype and Cmos14G0126400 associated with a miniature squash (mSq) phenotype”

✓ located verbatim in the source · abstract, characters 215–409 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In the mutant library, 15.5% of families showed visible phenotypic variation.

Taxon
Cucurbita moschataCucurbita moschatat0
Outcome
visible phenotypic variatione2
Value
15.5%e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucurbita moschata”

✓ located verbatim in the source · title, characters 113–131 · show in text ↑
e2

“families, with 15.5% showing visible phenotypic variation”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
Observationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The mutant library is a valuable resource for dissecting agronomic traits in Cucurbita moschata.

Taxon
Cucurbita moschataCucurbita moschatat0
Outcome
valuable resource for dissecting agronomic traitse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Schema note from the model: resource description; no comparator or value is stated

Verbatim

t0

“Cucurbita moschata”

✓ located verbatim in the source · title, characters 113–131 · show in text ↑
e3

“This library provides a valuable resource for dissecting agronomic traits and supports forward genetic approaches for identifying key genes in C. moschata”

✓ located verbatim in the source · abstract, characters 59–213 · show in text ↑
Observationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The PKUMo T2T genome assembly with the mutant library is a robust foundation for agronomic trait study and genetic improvement in Cucurbita breeding.

Taxon
Cucurbita moschataCucurbita moschatat0
Outcome
robust foundation for exploring agronomic traitse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Schema note from the model: resource description; no comparator or value is stated

Verbatim

t0

“Cucurbita moschata”

✓ located verbatim in the source · title, characters 113–131 · show in text ↑
e4

“the PKUMo T2T genome assembly, together with the extensive mutant library, provides a robust foundation for exploring agronomic traits and accelerating genetic improvement in Cucurbita breeding programs”

✓ located verbatim in the source · abstract, characters 420–622 · show in text ↑