Abstract
Regulation for labeling of food that includes genetically modified organism (GMO) has been developed and came into effect by a number of countries to enforce consumer's right to know.Many PCR based methods have already been introduced for routine detection or are currently under investigation for their performance in nucleic acid detection.However, for the development of DNA-based detection methods, taxon specific endogenous gene is absolutely imperative for quantification of GMOs as a reference gene and for qualitative detection as a positive control.In addition, to obtain reliable results, the detection must be compared with the corresponding taxon specific reference gene.In this study, the development of taxon specific eggplant -fructosidase gene primers for Loop Mediated Isothermal Amplification (LAMP), were described.Intra specific homogeneity and inter specific variability of -fructosidase gene of eggplant with potato and tomato obtained from public database were also analyzed, the limit of detection has obtained as 50 copies of the genome sequence.The results depicted the high specificity and sensitivity of the primers.Therefore, these primers of -fructosidase gene of eggplant can be used as a taxon specific endogenous reference gene for GMO analysis.
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Claims 4
Claim · model-written summary
Eggplant -fructosidase sequence showed within-species homogeneity and between-species variability relative to potato and tomato.
- Taxon
- eggplant
Solanum melongenae0 - Gene
- -fructosidase genee0
- Outcome
- Intra specific homogeneity and inter specific variabilitye0
- Comparator
- potato and tomatoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Intra specific homogeneity and inter specific variability of -fructosidase gene of eggplant with potato and tomato obtained from public database were also analyzed”
Claim · model-written summary
The eggplant LAMP primers had a detection limit of 50 genome copies.
- Taxon
- eggplantSolanum melongenae1
- Gene
- -fructosidase genee1
- Outcome
- limit of detectione0
- Value
- 50 copies of the genome sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the limit of detection has obtained as 50 copies of the genome sequence”
e1“taxon specific eggplant -fructosidase gene primers for Loop Mediated Isothermal Amplification (LAMP)”
Claim · model-written summary
The eggplant primers showed high specificity and sensitivity.
- Taxon
- eggplantSolanum melongenae1
- Gene
- -fructosidase genee1
- Outcome
- high specificity and sensitivitye0
- Value
- high specificity and sensitivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results depicted the high specificity and sensitivity of the primers”
e1“taxon specific eggplant -fructosidase gene primers for Loop Mediated Isothermal Amplification (LAMP)”
Claim · model-written summary
The authors propose the eggplant primers as an endogenous reference for GMO analysis.
- Taxon
- eggplantSolanum melongenae0
- Gene
- -fructosidase genee0
- Outcome
- taxon specific endogenous reference gene for GMO analysise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these primers of -fructosidase gene of eggplant can be used as a taxon specific endogenous reference gene for GMO analysis”