Abstract
Abstract Eelgrass (Zostera marina L.) is a typical seagrass species that plays a crucial role in marine ecosystems. Lateral organ boundaries (LOB) domain (LBD) genes, a family of plant-specific transcription factors characterized by the conserved LOB domain, are involved in the regulation of plant growth and development. However, this gene family has not yet been characterized in eelgrass. In this study, we performed a genome-wide identification, structural characterization, and expression analysis of LBD genes in Z. marina. A total of 23 ZosmaLBD genes (ZosmaLBD01 to ZosmaLBD23) were identified, which were unevenly distributed across six chromosomes. Phylogenetic analysis classified the ZosmaLBD family into two classes: Class I and Class II. Conserved motif and gene structure analyses revealed significant differences in the exon number, as well as in the count, type, and arrangement order of conserved motifs between Class I and Class II members. Promoter analysis predicted 49 types of cis-elements associated with plant growth and development, phytohormone responses, and abiotic and biotic stress responses in the promoters of ZosmaLBD genes. RNA-seq and qRT-PCR analyses showed that most ZosmaLBDs (14/23) were expressed in at least one tissue and exhibited diverse expression profiles. Notably, ZosmaLBD02, 17, and 20 in Class II were constitutively highly expressed in all tissues and were differentially expressed in most pairwise tissue comparisons, whereas ZosmaLBD01 and ZosmaLBD10 were specifically highly expressed in roots. These genes represent key candidates for further functional studies. Our findings provide a valuable foundation for understanding the potential roles of ZosmaLBDs in eelgrass growth and development.
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Claims 8
Claim · model-written summary
Twenty-three ZosmaLBD genes were identified across six chromosomes.
- Taxon
- Eelgrass
Zostera marinat0 - Gene
- ZosmaLBD genese0
- Outcome
- 23 ZosmaLBD genese0
- Value
- 23e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 23 ZosmaLBD genes (ZosmaLBD01 to ZosmaLBD23) were identified, which were unevenly distributed across six chromosomes.”
t0“Eelgrass”
Claim · model-written summary
The ZosmaLBD family was classified into two classes.
- Taxon
- EelgrassZostera marinat0
- Gene
- ZosmaLBD familye0
- Outcome
- two classes: Class I and Class IIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis classified the ZosmaLBD family into two classes: Class I and Class II.”
t0“Eelgrass”
Claim · model-written summary
Class I and II members differed in exon number and conserved motif characteristics.
- Taxon
- EelgrassZostera marinat0
- Outcome
- significant differences in the exon numbere0
- Qualifier
- count, type, and arrangement order of conserved motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Conserved motif and gene structure analyses revealed significant differences in the exon number, as well as in the count, type, and arrangement order of conserved motifs between Class I and Class II members.”
t0“Eelgrass”
Claim · model-written summary
Promoter analysis predicted 49 types of cis-elements linked to development and stress responses.
- Taxon
- EelgrassZostera marinat0
- Gene
- ZosmaLBD genese0
- Outcome
- 49 types of cis-elementse0
- Value
- 49 typese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter analysis predicted 49 types of cis-elements associated with plant growth and development, phytohormone responses, and abiotic and biotic stress responses in the promoters of ZosmaLBD genes.”
t0“Eelgrass”
Claim · model-written summary
Fourteen of 23 ZosmaLBD genes were expressed in at least one tissue.
- Taxon
- EelgrassZostera marinat0
- Gene
- ZosmaLBDse0
- Outcome
- expressed in at least one tissuee0
- Value
- 14/23e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most ZosmaLBDs (14/23) were expressed in at least one tissue and exhibited diverse expression profiles.”
t0“Eelgrass”
Claim · model-written summary
ZosmaLBD02, 17, and 20 were highly expressed across all tissues and differentially expressed in most tissue comparisons.
- Taxon
- EelgrassZostera marinat0
- Gene
- ZosmaLBD02, 17, and 20e0
- Outcome
- constitutively highly expressed in all tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZosmaLBD02, 17, and 20 in Class II were constitutively highly expressed in all tissues and were differentially expressed in most pairwise tissue comparisons, whereas ZosmaLBD01 and ZosmaLBD10 were specifically highly expressed in roots.”
t0“Eelgrass”
Claim · model-written summary
ZosmaLBD01 was highly expressed specifically in roots.
- Taxon
- EelgrassZostera marinat0
- Organ / stage
- rootse0
- Gene
- ZosmaLBD01e0
- Outcome
- specifically highly expressed in rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZosmaLBD01 and ZosmaLBD10 were specifically highly expressed in roots.”
t0“Eelgrass”
Claim · model-written summary
ZosmaLBD10 was highly expressed specifically in roots.
- Taxon
- EelgrassZostera marinat0
- Organ / stage
- rootse0
- Gene
- ZosmaLBD10e0
- Outcome
- specifically highly expressed in rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZosmaLBD01 and ZosmaLBD10 were specifically highly expressed in roots.”
t0“Eelgrass”