Abstract
BACKGROUND: The grapevine microbiome plays a central role in shaping vineyard performance, yet the influence of nursery inherited microbes on vineyard establishment and early productivity remains poorly understood. Our goals were to study the endosphere and rhizosphere microbiome succession as grapevine transition from nursery to vineyard and determine in what capacity the endogenous microbiome from the nursery shapes vineyard outcomes. RESULTS: We profiled, using amplicon-based sequencing, the fungal and bacterial communities across five bio-compartments (scion graft union, rootstock graft union, crown, roots, and rhizosphere) from two sets of grafted vines (Cabernet Sauvignon and Chardonnay grafted on 1103P rootstock) originating from two nurseries and followed their succession over three years after planting in a commercial vineyard. Nurseries produced vines with distinct endospheric microbiomes that converged overtime but that remained significantly different after three years. Microbial turnover occurred at a much faster pace in belowground (root and rhizosphere) compared to trunk compartments post-planting, with 15% of the initial microbes persisting in three-year-old vineyard. The fungal pathobiome partially inherited from nurseries and associated with vascular diseases of the trunk and root was also clearly distinct after three years. Yet, we did not observe typical disease symptoms development or vine death as we would expect, likely because vines were not under stress during the experimental timeframe. Vineyard yield was highly variable among clonal vines, and statistical modeling revealed that a narrow subset of amplicon sequence variants (ASVs) explained a large portion of this variance. Regression models using the top ten high-impact ASVs accounted for 51% and 60% of yield variation in trunk and belowground compartments, respectively. Notably, 16 of the 19 yield-associated ASVs originated from nurseries, underscoring the long-term influence of nursery-derived microbes on vineyard success. CONCLUSION: These findings highlight the dual role of beneficial and pathogenic nursery microbiota in shaping grapevine performance. It also suggests that the nursery life stage could be leveraged to engineer the grapevine microbiome and improve vineyard resilience.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Nurseries produced grapevines with distinct endospheric microbiomes that converged over time but remained significantly different after three years.
- Taxon
- vinese0
- Outcome
- endospheric microbiomese0
- Qualifier
- remained significantly different after three yearse0
Not reportedDirection
Verbatim
e0“Nurseries produced vines with distinct endospheric microbiomes that converged overtime but that remained significantly different after three years”
Claim · model-written summary
Microbial turnover after planting was much faster in belowground compartments (root and rhizosphere) than in trunk compartments.
- Organ / stage
- belowground (root and rhizosphere)e0
- Outcome
- Microbial turnovere0
- Direction
- fastere0
- Comparator
- trunk compartmentse0
Not reportedTaxon
Verbatim
e0“Microbial turnover occurred at a much faster pace in belowground (root and rhizosphere) compared to trunk compartments post-planting”
Claim · model-written summary
15% of the initial microbes persisted in the three-year-old vineyard.
Not reportedTaxonDirection
Verbatim
e0“with 15% of the initial microbes persisting in three-year-old vineyard”
Claim · model-written summary
The fungal pathobiome, partly inherited from nurseries and associated with vascular diseases of trunk and root, was also clearly distinct after three years.
Not reportedTaxon
Verbatim
e0“The fungal pathobiome partially inherited from nurseries and associated with vascular diseases of the trunk and root was also clearly distinct after three years”
Claim · model-written summary
No typical disease symptoms or vine death were observed during the experiment.
Not reportedDirection
Verbatim
e0“we did not observe typical disease symptoms development or vine death”
Claim · model-written summary
Vineyard yield was highly variable among clonal vines.
Not reportedDirection
Verbatim
e0“Vineyard yield was highly variable among clonal vines”
Claim · model-written summary
A narrow subset of amplicon sequence variants explained a large portion of the variance in vineyard yield among clonal vines.
Not reportedComparatorDirection
Verbatim
e0“Vineyard yield was highly variable among clonal vines, and statistical modeling revealed that a narrow subset of amplicon sequence variants (ASVs) explained a large portion of this variance”
Claim · model-written summary
Regression models using the top ten high-impact ASVs accounted for 51% of yield variation in trunk compartments.
Not reportedTaxonDirection
Verbatim
e0“Regression models using the top ten high-impact ASVs accounted for 51% and 60% of yield variation in trunk and belowground compartments, respectively”
Claim · model-written summary
Regression models using the top ten high-impact ASVs accounted for 60% of yield variation in belowground compartments.
Not reportedTaxonDirection
Verbatim
e0“Regression models using the top ten high-impact ASVs accounted for 51% and 60% of yield variation in trunk and belowground compartments, respectively”
Claim · model-written summary
16 of the 19 yield-associated ASVs originated from nurseries.
Not reportedTaxon
Verbatim
e0“16 of the 19 yield-associated ASVs originated from nurseries”
Claim · model-written summary
The authors suggest that the nursery life stage could be leveraged to engineer the grapevine microbiome and improve vineyard resilience.
- Outcome
- engineer the grapevine microbiome and improve vineyard resiliencee0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nursery life stage could be leveraged to engineer the grapevine microbiome and improve vineyard resilience”