Paper · 2026 · Journal of Advances in Biology & Biotechnology

Nitrogen Driven Zinc Biofortification in Wheat (Triticum aestivum L.): Linking Growth, Phenology and Yield Attributes

Aditi Mehra; Y. A. Tamboli; Ajeet Singh; et al.

11
claims
28
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

Wheat (Triticum aestivum L.) is one of the most important cereal crops, serving as a staple food for nearly two-thirds of the global population and ranking second after rice in the developing world. Agronomic biofortification through zinc fertilization, particularly when combined with optimal nitrogen management, has emerged as an effective strategy to enhance both crop productivity and grain nutritional quality. The synergistic interaction between nitrogen and zinc plays a crucial role in improving nutrient uptake, growth, and yield attributes of wheat, especially under Zn-deficient soil conditions. A field experiment was conducted during the rabi season of 2025-26 at the Agronomy Research Farm, School of Agricultural Sciences, Jaipur National University, Jaipur, Rajasthan to evaluate the effect of zinc bio-fortification under varying nitrogen levels on the growth and yield attributes of wheat. The experiment was laid out in a randomized block design with three replications, comprising ten treatments ranging from control (T₁) to different nitrogen and zinc combinations. The results indicated that growth parameters were significantly influenced by the treatments with 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉) recorded the maximum plant height (89.86 cm), number of tillers plant⁻¹ (7.68) and dry matter accumulation (14.16 g plant⁻¹). This treatment remained statistically at par with T₁₀, T₈ and T₆ and exhibited increases of 29.91%, 44.09% and 46.74% over control (T₁), respectively. However, plant population at 20 DAS and at maturity was not significantly affected by the treatments. Phenological parameters were also influenced with the maximum days to 50% flowering (82.53) and physiological maturity (128.50) recorded under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉). Similarly, yield attributes were significantly improved under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉) recorded the highest spike length (11.34 cm), grains spike⁻¹ (51.38), seed yield plant⁻¹ (32.49 g) and test weight (43.50 g) with corresponding increases of 50.80%, 20.52%, 46.50% and 16.59% over control. Thus, the application of 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉) proved to be the most effective treatment for enhancing growth, yield attributes and overall productivity of wheat.

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Claims 11

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T9 gave maximum plant height of 89.86 cm, 29.91% over control.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e0
Outcome
plant heighte0
Direction
maximume0
Value
89.86 cme0
Qualifier
29.91%e1
Comparator
control (T₁)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉) recorded the maximum plant height (89.86 cm), number of tillers plant⁻¹ (7.68) and dry matter accumulation (14.16 g plant⁻¹)”

✓ located verbatim in the source · abstract, characters 1186–1345 · show in text ↑
e1

“increases of 29.91%, 44.09% and 46.74% over control (T₁)”

✓ located verbatim in the source · abstract, characters 1426–1482 · show in text ↑
e2

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T9 gave maximum tillers per plant of 7.68, 44.09% over control.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e0
Outcome
number of tillers plant⁻¹e0
Direction
maximume0
Value
7.68e0
Qualifier
44.09%e1
Comparator
control (T₁)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉) recorded the maximum plant height (89.86 cm), number of tillers plant⁻¹ (7.68) and dry matter accumulation (14.16 g plant⁻¹)”

✓ located verbatim in the source · abstract, characters 1186–1345 · show in text ↑
e1

“increases of 29.91%, 44.09% and 46.74% over control (T₁)”

✓ located verbatim in the source · abstract, characters 1426–1482 · show in text ↑
e2

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T9 gave maximum dry matter of 14.16 g per plant, 46.74% over control.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e0
Outcome
dry matter accumulatione0
Direction
maximume0
Value
14.16 g plant⁻¹e0
Qualifier
46.74%e1
Comparator
control (T₁)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉) recorded the maximum plant height (89.86 cm), number of tillers plant⁻¹ (7.68) and dry matter accumulation (14.16 g plant⁻¹)”

✓ located verbatim in the source · abstract, characters 1186–1345 · show in text ↑
e1

“increases of 29.91%, 44.09% and 46.74% over control (T₁)”

✓ located verbatim in the source · abstract, characters 1426–1482 · show in text ↑
e2

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Plant population at 20 DAS and maturity was not significantly affected.

Treatment
treatmentse0
Outcome
plant population at 20 DAS and at maturitye0
Direction
not significantly affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“plant population at 20 DAS and at maturity was not significantly affected by the treatments”

✓ located verbatim in the source · abstract, characters 1507–1598 · show in text ↑
e1

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

T9 produced maximum days to 50% flowering of 82.53.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e0
Outcome
days to 50% floweringe0
Direction
maximume0
Value
82.53e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“maximum days to 50% flowering (82.53) and physiological maturity (128.50) recorded under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉)”

✓ located verbatim in the source · abstract, characters 1654–1777 · show in text ↑
e1

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

T9 produced maximum days to physiological maturity of 128.50.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e0
Outcome
physiological maturitye0
Direction
maximume0
Value
128.50e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“maximum days to 50% flowering (82.53) and physiological maturity (128.50) recorded under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉)”

✓ located verbatim in the source · abstract, characters 1654–1777 · show in text ↑
e1

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T9 gave spike length of 11.34 cm, 50.80% over control.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e1
Outcome
spike lengthe0
Direction
higheste0
Value
11.34 cme0
Qualifier
50.80%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“highest spike length (11.34 cm), grains spike⁻¹ (51.38), seed yield plant⁻¹ (32.49 g) and test weight (43.50 g) with corresponding increases of 50.80%, 20.52%, 46.50% and 16.59% over control”

✓ located verbatim in the source · abstract, characters 1889–2079 · show in text ↑
e1

“recorded under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉)”

✓ located verbatim in the source · abstract, characters 1728–1777 · show in text ↑
e2

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T9 gave 51.38 grains per spike, 20.52% over control.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e1
Outcome
grains spike⁻¹e0
Direction
higheste0
Value
51.38e0
Qualifier
20.52%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“highest spike length (11.34 cm), grains spike⁻¹ (51.38), seed yield plant⁻¹ (32.49 g) and test weight (43.50 g) with corresponding increases of 50.80%, 20.52%, 46.50% and 16.59% over control”

✓ located verbatim in the source · abstract, characters 1889–2079 · show in text ↑
e1

“recorded under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉)”

✓ located verbatim in the source · abstract, characters 1728–1777 · show in text ↑
e2

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T9 gave seed yield of 32.49 g per plant, 46.50% over control.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e1
Outcome
seed yield plant⁻¹e0
Direction
higheste0
Value
32.49 ge0
Qualifier
46.50%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“highest spike length (11.34 cm), grains spike⁻¹ (51.38), seed yield plant⁻¹ (32.49 g) and test weight (43.50 g) with corresponding increases of 50.80%, 20.52%, 46.50% and 16.59% over control”

✓ located verbatim in the source · abstract, characters 1889–2079 · show in text ↑
e1

“recorded under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉)”

✓ located verbatim in the source · abstract, characters 1728–1777 · show in text ↑
e2

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T9 gave test weight of 43.50 g, 16.59% over control.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e1
Outcome
test weighte0
Direction
higheste0
Value
43.50 ge0
Qualifier
16.59%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“highest spike length (11.34 cm), grains spike⁻¹ (51.38), seed yield plant⁻¹ (32.49 g) and test weight (43.50 g) with corresponding increases of 50.80%, 20.52%, 46.50% and 16.59% over control”

✓ located verbatim in the source · abstract, characters 1889–2079 · show in text ↑
e1

“recorded under 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉)”

✓ located verbatim in the source · abstract, characters 1728–1777 · show in text ↑
e2

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · abstract, characters 0–28 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors considered T9 the most effective treatment for wheat productivity.

Treatment
125% RDN + ZnSO₄ @ 25 kg ha⁻¹e0
Outcome
most effective treatment for enhancing growth, yield attributes and overall productivitye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“application of 125% RDN + ZnSO₄ @ 25 kg ha⁻¹ (T₉) proved to be the most effective treatment for enhancing growth, yield attributes and overall productivity of wheat”

✓ located verbatim in the source · abstract, characters 2091–2255 · show in text ↑

zinc sulfate · growth · Triticum aestivum (wheat)Results differ

33 papers: 30 positive, 3 negative or no effect

29 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. negative

29 papers more in this group

See all 33 papers →

zinc sulfate · yield · Triticum aestivum (wheat)Results differ

100 papers: 98 positive, 2 negative or no effect

97 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. positive

96 papers more in this group

See all 100 papers →

zinc sulfate · quality: grain · Triticum aestivum (wheat)Results agree

10 papers: 10 positive, 0 negative or no effect

9 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.