Paper · 2026 · MethodsX

A rapid and simplified technique for inducing Ganoderma lucidum infections in coconut and arecanut seedlings

Daliyamol; K.K. Greena; V.H. Prathibha; et al.

3
claims
6
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

Ganoderma lucidum is a soil borne fungi known to cause Basal stem rot (BSR) or Thanjavur wilt or Ganoderma wilt disease in coconut as well as Foot rot or Anabe roga disease in arecanut plantations. Due to its soil borne nature, effectively managing the disease in field conditions is highly challenging. To combat this issue, the development of resistant or tolerant seedlings is crucial for sustainable production of coconut and arecanut palms. Therefore, a reliable and rapid method to assess resistance of palms to Ganoderma disease is essential. Here we report a rapid inoculation technique wherein seedlings were soaked in 5 days old mycelia cultures supplemented with 0.002% Tween 20 for a period of 60 minutes. The initial symptoms could be noticed 15 days after inoculation in arecanut and 30 days after inoculation in coconut. The technique ensures consistent infection, allowing disease evaluation as early as two weeks post inoculation with G. lucidum . Our results indicate that this new inoculation technique can be used routinely to screen coconut and arecanut seedlings for BSR disease and to evaluate the resistance of different cultivars to G. lucidum. • Developed a rapid, simple and efficient inoculation technique • The technique was evaluated for coconut and arecanut seedlings as well as tissue culture plantlets

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 3

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The rapid inoculation technique ensures consistent infection, allowing evaluation of Ganoderma disease in coconut and arecanut seedlings as early as two weeks after inoculation.

Taxon
coconut and arecanut seedlingst0
Interactor
G. lucidumGanoderma lucidume0pathogen
Outcome
consistent infectione0
Value
two weeks post inoculatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ganoderma lucidum
Ganoderma lucidum · InteractorEric Steinert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ganoderma lucidum infections in coconut and arecanut seedlings”

✓ located verbatim in the source · title, characters 46–108 · show in text ↑
e0

“The technique ensures consistent infection, allowing disease evaluation as early as two weeks post inoculation with G. lucidum”

✓ located verbatim in the source · abstract, characters 836–962 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Initial Ganoderma lucidum symptoms were noticed 15 days after inoculation in arecanut seedlings.

Taxon
arecanutAreca catechue0
Interactor
Ganoderma lucidumGanoderma lucidumt0pathogen
Outcome
initial symptomse0
Value
15 days after inoculatione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Areca catechu
Areca catechu · TaxonPratheepps · CC BY-SA 2.5 · Wikimedia Commons ↗
Ganoderma lucidum
Ganoderma lucidum · InteractorEric Steinert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ganoderma lucidum infections in coconut and arecanut seedlings”

✓ located verbatim in the source · title, characters 46–108 · show in text ↑
e0

“The initial symptoms could be noticed 15 days after inoculation in arecanut”

✓ located verbatim in the source · abstract, characters 718–793 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Initial Ganoderma lucidum symptoms were noticed 30 days after inoculation in coconut seedlings.

Taxon
coconute0
Interactor
Ganoderma lucidumGanoderma lucidumt0pathogen
Outcome
initial symptomse0
Value
30 days after inoculatione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ganoderma lucidum
Ganoderma lucidum · InteractorEric Steinert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ganoderma lucidum infections in coconut and arecanut seedlings”

✓ located verbatim in the source · title, characters 46–108 · show in text ↑
e0

“The initial symptoms could be noticed 15 days after inoculation in arecanut and 30 days after inoculation in coconut”

✓ located verbatim in the source · abstract, characters 718–834 · show in text ↑