Abstract
Ganoderma lucidum is a soil borne fungi known to cause Basal stem rot (BSR) or Thanjavur wilt or Ganoderma wilt disease in coconut as well as Foot rot or Anabe roga disease in arecanut plantations. Due to its soil borne nature, effectively managing the disease in field conditions is highly challenging. To combat this issue, the development of resistant or tolerant seedlings is crucial for sustainable production of coconut and arecanut palms. Therefore, a reliable and rapid method to assess resistance of palms to Ganoderma disease is essential. Here we report a rapid inoculation technique wherein seedlings were soaked in 5 days old mycelia cultures supplemented with 0.002% Tween 20 for a period of 60 minutes. The initial symptoms could be noticed 15 days after inoculation in arecanut and 30 days after inoculation in coconut. The technique ensures consistent infection, allowing disease evaluation as early as two weeks post inoculation with G. lucidum . Our results indicate that this new inoculation technique can be used routinely to screen coconut and arecanut seedlings for BSR disease and to evaluate the resistance of different cultivars to G. lucidum. • Developed a rapid, simple and efficient inoculation technique • The technique was evaluated for coconut and arecanut seedlings as well as tissue culture plantlets
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Claims 3
Claim · model-written summary
The rapid inoculation technique ensures consistent infection, allowing evaluation of Ganoderma disease in coconut and arecanut seedlings as early as two weeks after inoculation.
- Taxon
- coconut and arecanut seedlingst0
- Interactor
- G. lucidum
Ganoderma lucidume0pathogen - Outcome
- consistent infectione0
- Value
- two weeks post inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ganoderma lucidum infections in coconut and arecanut seedlings”
e0“The technique ensures consistent infection, allowing disease evaluation as early as two weeks post inoculation with G. lucidum”
Claim · model-written summary
Initial Ganoderma lucidum symptoms were noticed 15 days after inoculation in arecanut seedlings.
- Taxon
- arecanut
Areca catechue0 - Interactor
- Ganoderma lucidum
Ganoderma lucidumt0pathogen - Outcome
- initial symptomse0
- Value
- 15 days after inoculatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
t0“Ganoderma lucidum infections in coconut and arecanut seedlings”
e0“The initial symptoms could be noticed 15 days after inoculation in arecanut”
Claim · model-written summary
Initial Ganoderma lucidum symptoms were noticed 30 days after inoculation in coconut seedlings.
- Taxon
- coconute0
- Interactor
- Ganoderma lucidum
Ganoderma lucidumt0pathogen - Outcome
- initial symptomse0
- Value
- 30 days after inoculatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ganoderma lucidum infections in coconut and arecanut seedlings”
e0“The initial symptoms could be noticed 15 days after inoculation in arecanut and 30 days after inoculation in coconut”