Abstract
Thinopyrum elongatum (2 n = 4 x = 28) harbors multiple valuable resistance genes and serves as a valuable genetic resource for wheat improvement. Yr1EL was primarily identified on chromosome arm 1EL of tetraploid Th. elongatum , conferring adult-plant resistance to stripe rust. To further map and utilize Yr1EL , we introduced chromosomal rearrangements by crossing the wheat–tetraploid Th. elongatum 1E(1D) substitution line and the T1BS·1EL translocation line with the common wheat Chinese Spring ph1b mutant. In total, eight wheat– Th. elongatum chromosome 1E structural variants were identified by in situ hybridization, the GenoBaits®WheatplusEE panel, and molecular markers. These variants include one large segment translocation, one terminal fragment deletion, one chromosome 1E insertion translocation, and five chromosome 1EL terminal small fragment translocations. Based on phenotyping and genotyping of these variant lines, Yr1EL was mapped to an approximately 20.91 Mb physical interval (532.45 to 553.35 Mb) on the distal long arm of chromosome 1E corresponding to the diploid Th. elongatum reference genome . Genetic analysis confirmed that stripe rust resistance was conferred by the Yr1EL locus with incomplete dominance. Five codominant molecular markers co-segregated with the Yr1EL locus and will facilitate marker-assisted selection . Furthermore, collinearity analysis showed that this interval is structurally conserved among Triticeae species, and 29 candidate genes potentially related to disease resistance were annotated. Those candidates included receptor-like proteins, kinases, nucleotide-binding and leucine-rich repeat receptors, and other disease resistance proteins. The adult-plant resistance gene Yr1EL expands the wheat disease-resistance gene pool and provides valuable germplasm for breeding durable stripe rust-resistant cultivars.
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Claims 8
Claim · model-written summary
Eight wheat–Thinopyrum elongatum 1E structural variants were identified.
- Taxon
- wheat
Triticum aestivume1 - Outcome
- chromosome 1E structural variantse1
- Value
- eighte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“eight wheat– Th. elongatum chromosome 1E structural variants were identified”
Claim · model-written summary
The variants include one large segment translocation, one terminal fragment deletion and one chromosome 1E insertion translocation.
- Taxon
- wheatTriticum aestivume1
- Outcome
- one large segment translocation, one terminal fragment deletion, one chromosome 1E insertion translocatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“eight wheat– Th. elongatum chromosome 1E structural variants were identified”
e2“These variants include one large segment translocation, one terminal fragment deletion, one chromosome 1E insertion translocation, and five chromosome 1EL terminal small fragment translocations”
Claim · model-written summary
Five chromosome 1EL terminal small fragment translocations were among the variants.
- Taxon
- wheatTriticum aestivume1
- Outcome
- five chromosome 1EL terminal small fragment translocationse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“eight wheat– Th. elongatum chromosome 1E structural variants were identified”
e2“These variants include one large segment translocation, one terminal fragment deletion, one chromosome 1E insertion translocation, and five chromosome 1EL terminal small fragment translocations”
Claim · model-written summary
Yr1EL was mapped to a physical interval of about 20.91 Mb on the distal long arm of chromosome 1E.
- Taxon
- Thinopyrum elongatum
Thinopyrum elongatume0 - Gene
- Yr1ELe4
- Outcome
- physical intervale4
- Value
- 20.91 Mbe4
- Qualifier
- distal long arm of chromosome 1Ee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thinopyrum elongatum (2 n = 4 x = 28)”
e4“Yr1EL was mapped to an approximately 20.91 Mb physical interval (532.45 to 553.35 Mb) on the distal long arm of chromosome 1E”
Claim · model-written summary
Stripe rust resistance was conferred by the Yr1EL locus with incomplete dominance.
- Taxon
- Thinopyrum elongatumThinopyrum elongatume0
- Interactor
- stripe ruste5pathogen
- Gene
- Yr1ELe5
- Outcome
- stripe rust resistancee5
- Qualifier
- incomplete dominancee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thinopyrum elongatum (2 n = 4 x = 28)”
e5“Genetic analysis confirmed that stripe rust resistance was conferred by the Yr1EL locus with incomplete dominance”
Claim · model-written summary
Five codominant molecular markers co-segregated with the Yr1EL locus.
- Taxon
- Thinopyrum elongatumThinopyrum elongatume0
- Gene
- Yr1ELe6
- Outcome
- co-segregated with the Yr1EL locuse6
- Value
- Fivee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thinopyrum elongatum (2 n = 4 x = 28)”
e6“Five codominant molecular markers co-segregated with the Yr1EL locus”
Claim · model-written summary
The Yr1EL interval is structurally conserved among Triticeae species according to collinearity analysis.
- Taxon
- Thinopyrum elongatumThinopyrum elongatume0
- Outcome
- structurally conserved among Triticeae speciese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thinopyrum elongatum (2 n = 4 x = 28)”
e7“collinearity analysis showed that this interval is structurally conserved among Triticeae species”
Claim · model-written summary
29 candidate genes potentially related to disease resistance were annotated in the interval.
- Taxon
- Thinopyrum elongatumThinopyrum elongatume0
- Outcome
- candidate genes potentially related to disease resistancee3
- Value
- 29e3
- Qualifier
- receptor-like proteins, kinases, nucleotide-binding and leucine-rich repeat receptorse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thinopyrum elongatum (2 n = 4 x = 28)”
e3“29 candidate genes potentially related to disease resistance were annotated. Those candidates included receptor-like proteins, kinases, nucleotide-binding and leucine-rich repeat receptors, and other disease resistance proteins”