Abstract
Plants have evolved numerous belowground strategies to capture nutrients. While root functional differentiation between absorption and transportation has been inferred from order-based traits, the role of microbiomes in mediating this differentiation remains unclear. We measured traits (anatomical, chemical, and morphological) of lower order (absorption-dominated) and higher order (transportation-dominated) roots of 37 herbaceous species (13 monocots and 24 dicots) in a temperate grassland. Furthermore, we employed high-throughput quantitative polymerase chain reaction and 16S rRNA gene sequencing to investigate bacterial nitrogen-transformation gene abundance, diversity, and community assembly along the soil-root continuum (rhizosphere, rhizoplane, and endosphere) and analyzed their relationships with root traits. Monocot roots exhibited greater bacterial diversity and nitrogen-transformation gene abundances than dicots. Within dicots, lower order roots showed higher bacterial diversity and nitrogen-transformation gene abundances than higher order roots, a pattern not observed in monocots. Lower order roots, characterized by higher cortex proportion, facilitated the enrichment of diverse bacteria and recruitment of nitrogen-transformation microorganisms. These patterns were associated with a decrease in homogeneous selection from lower order to higher order roots. This study reveals the mechanisms of functional differences among herbaceous root orders from a microbiome perspective, offering further insights into how root-microbe interactions underpin nitrogen-transformation potential in terrestrial ecosystems.
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Claims 8
Claim · model-written summary
Monocot roots had greater bacterial diversity than dicots.
Verbatim
e0“Monocot roots exhibited greater bacterial diversity and nitrogen-transformation gene abundances than dicots.”
Claim · model-written summary
Monocot roots had greater nitrogen-transformation gene abundances than dicots.
Verbatim
e0“Monocot roots exhibited greater bacterial diversity and nitrogen-transformation gene abundances than dicots.”
Claim · model-written summary
Lower order dicot roots had higher bacterial diversity than higher order roots.
Verbatim
e0“Within dicots, lower order roots showed higher bacterial diversity and nitrogen-transformation gene abundances than higher order roots, a pattern not observed in monocots.”
Claim · model-written summary
Lower order dicot roots had higher nitrogen-transformation gene abundances than higher order roots.
Verbatim
e0“Within dicots, lower order roots showed higher bacterial diversity and nitrogen-transformation gene abundances than higher order roots, a pattern not observed in monocots.”
Claim · model-written summary
The root order pattern was absent in monocots.
- Taxon
- monocotsLiliopsidae0
- Outcome
- pattern not observede0
Verbatim
e0“a pattern not observed in monocots.”
Claim · model-written summary
Lower order roots facilitated enrichment of diverse bacteria.
Verbatim
e0“Lower order roots, characterized by higher cortex proportion, facilitated the enrichment of diverse bacteria and recruitment of nitrogen-transformation microorganisms.”
e1“37 herbaceous species”
Claim · model-written summary
Lower order roots facilitated recruitment of nitrogen-transformation microorganisms.
Verbatim
e0“Lower order roots, characterized by higher cortex proportion, facilitated the enrichment of diverse bacteria and recruitment of nitrogen-transformation microorganisms.”
e1“37 herbaceous species”
Claim · model-written summary
Homogeneous selection decreased from lower to higher order roots.
Verbatim
e0“These patterns were associated with a decrease in homogeneous selection from lower order to higher order roots.”
e1“37 herbaceous species”